Comprehensive Evaluation of Neuroprotective Effects of Levetiracetam in C57BL/6J Male Mice Model of Parkinsonism: Preclinical Insights
Introduction: Levetiracetam (LEV), an anticonvulsant used for epilepsy, exhibits neuroprotective effects by stabilising neuronal activity and reducing excitotoxicity. Parkinsonism, caused by the degeneration of dopaminergic neurons, is primarily managed symptomatically. LEV may offer both symptom re...
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JCDR Research and Publications Private Limited
2025-06-01
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| author | Neeraj Pandey VP Karthik Preetha Selva Philo Hazeena |
| author_facet | Neeraj Pandey VP Karthik Preetha Selva Philo Hazeena |
| author_sort | Neeraj Pandey |
| collection | DOAJ |
| description | Introduction: Levetiracetam (LEV), an anticonvulsant used for epilepsy, exhibits neuroprotective effects by stabilising neuronal activity and reducing excitotoxicity. Parkinsonism, caused by the degeneration of dopaminergic neurons, is primarily managed symptomatically. LEV may offer both symptom relief and neuroprotection, presenting a potential alternative therapy.
Aim: This study aimed to evaluate the neuroprotective effects of LEV in a mouse model of Parkinsonism. It investigated whether LEV, an antiepileptic drug, can mitigate the neurodegenerative processes and improve motor function and oxidative stress markers in a mouse model induced with Parkinsonism using 1-Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine (MPTP), a neurotoxin that selectively destroys dopaminergic neurons.
Materials and Methods: The present experimental study was conducted at the Department of Pharmacology, Sri Ramachandra Medical College and Research Institute, SRIHER, Chennai, Tamil Nadu, India, from October 2023 to March 2024. Thirty-six male mice were divided were divided into six groups: control (vehicle), control (MPTP-treated), LEV high dose, L-Dopa + MPTP, LEV low dose + MPTP, and LEV high dose + MPTP. The mice underwent various behavioural tests, including the Open Field Test (OFT), rota-rod test, and foot slips test. Biochemical assays, such as Superoxide Dismutase (SOD), Glutathione Peroxidase (GPx), and nitrite levels, were performed to assess oxidative stress and antioxidant defences.
Results: The LEV-treated groups showed significant improvements (p<0.05) in locomotor activity, motor coordination, and exploratory behaviour compared to the MPTP-treated control group. LEV at a high dose of 54 mg/kg significantly enhanced antioxidant enzyme levels, with SOD at 0.373 U/mg, GPX at 3.436 mcg/mg/min, and Nitric Oxide (NO) at 3.482 mg/mL, indicating its neuroprotective potential.
Conclusion: LEV demonstrated significant neuroprotective effects in a mouse model of Parkinsonism. The improvements in both behavioural outcomes and biochemical markers suggest its potential as a therapeutic agent for neurodegenerative diseases. |
| format | Article |
| id | doaj-art-d4026edf49464454941c1f93fa04dcd0 |
| institution | OA Journals |
| issn | 2249-782X 0973-709X |
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| publishDate | 2025-06-01 |
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| spelling | doaj-art-d4026edf49464454941c1f93fa04dcd02025-08-20T02:22:03ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2025-06-01196FC24FC3010.7860/JCDR/2025/78708.21141Comprehensive Evaluation of Neuroprotective Effects of Levetiracetam in C57BL/6J Male Mice Model of Parkinsonism: Preclinical InsightsNeeraj Pandey0VP Karthik1Preetha Selva2Philo Hazeena3Ph.D. Scholar, Department of Pharmacology, Sri Ramachandra Institute of Higher Education and Research, Chennai, Tamil Nadu, India.Associate Professor, Department of Pharmacology, Sri Ramachandra Institute of Higher Education and Research, Chennai, Tamil Nadu, India.Professor, Department of Pharmacology, Saveetha Medical College and Hospital, Saveetha Institute of Medical and Technical Sciences, Chennai, Tamil Nadu, India.Associate Professor, Department of Neurology, Sri Ramachandra Institute of Higher Education and Research, Chennai, Tamil Nadu, India.Introduction: Levetiracetam (LEV), an anticonvulsant used for epilepsy, exhibits neuroprotective effects by stabilising neuronal activity and reducing excitotoxicity. Parkinsonism, caused by the degeneration of dopaminergic neurons, is primarily managed symptomatically. LEV may offer both symptom relief and neuroprotection, presenting a potential alternative therapy. Aim: This study aimed to evaluate the neuroprotective effects of LEV in a mouse model of Parkinsonism. It investigated whether LEV, an antiepileptic drug, can mitigate the neurodegenerative processes and improve motor function and oxidative stress markers in a mouse model induced with Parkinsonism using 1-Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine (MPTP), a neurotoxin that selectively destroys dopaminergic neurons. Materials and Methods: The present experimental study was conducted at the Department of Pharmacology, Sri Ramachandra Medical College and Research Institute, SRIHER, Chennai, Tamil Nadu, India, from October 2023 to March 2024. Thirty-six male mice were divided were divided into six groups: control (vehicle), control (MPTP-treated), LEV high dose, L-Dopa + MPTP, LEV low dose + MPTP, and LEV high dose + MPTP. The mice underwent various behavioural tests, including the Open Field Test (OFT), rota-rod test, and foot slips test. Biochemical assays, such as Superoxide Dismutase (SOD), Glutathione Peroxidase (GPx), and nitrite levels, were performed to assess oxidative stress and antioxidant defences. Results: The LEV-treated groups showed significant improvements (p<0.05) in locomotor activity, motor coordination, and exploratory behaviour compared to the MPTP-treated control group. LEV at a high dose of 54 mg/kg significantly enhanced antioxidant enzyme levels, with SOD at 0.373 U/mg, GPX at 3.436 mcg/mg/min, and Nitric Oxide (NO) at 3.482 mg/mL, indicating its neuroprotective potential. Conclusion: LEV demonstrated significant neuroprotective effects in a mouse model of Parkinsonism. The improvements in both behavioural outcomes and biochemical markers suggest its potential as a therapeutic agent for neurodegenerative diseases.https://jcdr.net/articles/PDF/21141/78708_CE[Ra1]__F(KR)_QC(AN_SS)_PF1(Rf_OM)_PFA(IS)_PB(Rf_IS)_PN(IS).pdfantioxidantdopaminergic neurons1-methyl-4-phenyl-1236-tetrahydropyridineneurodegenerative |
| spellingShingle | Neeraj Pandey VP Karthik Preetha Selva Philo Hazeena Comprehensive Evaluation of Neuroprotective Effects of Levetiracetam in C57BL/6J Male Mice Model of Parkinsonism: Preclinical Insights Journal of Clinical and Diagnostic Research antioxidant dopaminergic neurons 1-methyl-4-phenyl-1 2 3 6-tetrahydropyridine neurodegenerative |
| title | Comprehensive Evaluation of Neuroprotective Effects of Levetiracetam in C57BL/6J Male Mice Model of Parkinsonism: Preclinical Insights |
| title_full | Comprehensive Evaluation of Neuroprotective Effects of Levetiracetam in C57BL/6J Male Mice Model of Parkinsonism: Preclinical Insights |
| title_fullStr | Comprehensive Evaluation of Neuroprotective Effects of Levetiracetam in C57BL/6J Male Mice Model of Parkinsonism: Preclinical Insights |
| title_full_unstemmed | Comprehensive Evaluation of Neuroprotective Effects of Levetiracetam in C57BL/6J Male Mice Model of Parkinsonism: Preclinical Insights |
| title_short | Comprehensive Evaluation of Neuroprotective Effects of Levetiracetam in C57BL/6J Male Mice Model of Parkinsonism: Preclinical Insights |
| title_sort | comprehensive evaluation of neuroprotective effects of levetiracetam in c57bl 6j male mice model of parkinsonism preclinical insights |
| topic | antioxidant dopaminergic neurons 1-methyl-4-phenyl-1 2 3 6-tetrahydropyridine neurodegenerative |
| url | https://jcdr.net/articles/PDF/21141/78708_CE[Ra1]__F(KR)_QC(AN_SS)_PF1(Rf_OM)_PFA(IS)_PB(Rf_IS)_PN(IS).pdf |
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