Genome-Wide Identification, Classification, Expression Analysis, and Screening of Drought and Heat Resistance-Related Candidates of the <i>Rboh</i> Gene Family in Wheat

Genome-Wide Identification, Classification, Expression Analysis, and Screening of Drought and Heat Resistance-Related Candidates of the <i>Rboh</i> Gene Family in Wheat

Plant respiratory burst oxidase homologs (Rbohs) are key enzymes that produce reactive oxygen species (ROS), which serve as signaling molecules regulating plant growth and stress responses. In this study, 39 <i>TaRboh</i> genes (<i>TaRboh01</i>–<i>TaRboh39</i>) we...

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Main Authors: Miyuan Cao, Yue Zhang, Xiaoxiao Zou, Huangping Yin, Yan Yin, Zeqi Li, Wenjun Xiao, Shucan Liu, Yongliang Li, Xinhong Guo
Format: Article
Language:English
Published: MDPI AG 2024-11-01
Series:Plants
Subjects:
<i>Triticum aestivum</i>
<i>Rboh</i>s
abiotic stress
reactive oxygen species
expression patterns
Online Access:https://www.mdpi.com/2223-7747/13/23/3377
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Summary:Plant respiratory burst oxidase homologs (Rbohs) are key enzymes that produce reactive oxygen species (ROS), which serve as signaling molecules regulating plant growth and stress responses. In this study, 39 <i>TaRboh</i> genes (<i>TaRboh01</i>–<i>TaRboh39</i>) were identified. These genes were distributed unevenly among the wheat genome’s fourteen chromosomes, with the exception of homoeologous group 2 and 7 and chromosomes 4A, as well as one unidentified linkage group (Un). TaRbohs were classified into ten distinct clades, each sharing similar motif compositions and gene structures. The promoter regions of <i>TaRboh</i>s contained <i>cis</i>-elements related to hormones, growth and development, and stresses. Furthermore, five <i>TaRboh</i> genes (<i>TaRboh26</i>, <i>TaRboh27</i>, <i>TaRboh31</i>, <i>TaRboh32</i>, and <i>TaRboh34</i>) exhibited strong evolutionary conservation. Additionally, a Ka/Ks analysis confirmed that purifying selection was the predominant force driving the evolution of these genes. Expression profiling and qPCR results further indicated differential expression patterns of <i>TaRboh</i> genes between heat and drought stresses. <i>TaRboh11</i>, <i>TaRboh20</i>, <i>TaRboh22</i>, <i>TaRboh24</i>, <i>TaRboh29</i>, and <i>TaRboh34</i> were significantly upregulated under multiple stress conditions, whereas <i>TaRboh30</i> was only elevated in response to drought stress. Collectively, our findings provide a systematic analysis of the wheat <i>Rboh</i> gene family and establish a theoretical framework for our future research on the role of <i>Rboh</i> genes in response to heat and drought stress.
ISSN:2223-7747

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