In Vivo Evaluation of the Anti-Skin-Ageing Bioactivity of a Recombinant Dual Humanised Collagen and Poly-L-Lactic Acid
This study introduces a novel recombinant humanised collagen (DuCol) developed through codon optimisation and prokaryotic soluble expression, exhibiting exceptional biocompatibility and bioactivity. Structural integrity was confirmed via RP-HPLC, SEM, and CD spectroscopy. In vitro evaluations reveal...
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2025-05-01
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| Online Access: | https://www.mdpi.com/2306-5354/12/5/510 |
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| author | Mingjie Tong Xin Zhou Jiongni Zhong Dengjian Qu Wei Chen Chun Chen Yiting Wang Yaoping Liu Shaochuan Li Yuan Xiao Ning Wang Chaowan Guo Qiuling Xie Sheng Xiong |
| author_facet | Mingjie Tong Xin Zhou Jiongni Zhong Dengjian Qu Wei Chen Chun Chen Yiting Wang Yaoping Liu Shaochuan Li Yuan Xiao Ning Wang Chaowan Guo Qiuling Xie Sheng Xiong |
| author_sort | Mingjie Tong |
| collection | DOAJ |
| description | This study introduces a novel recombinant humanised collagen (DuCol) developed through codon optimisation and prokaryotic soluble expression, exhibiting exceptional biocompatibility and bioactivity. Structural integrity was confirmed via RP-HPLC, SEM, and CD spectroscopy. In vitro evaluations revealed DuCol’s dose-dependent enhancement of NIH-3T3 fibroblast proliferation, adhesion, and migration. In a D-galactose-induced ageing rat model, subcutaneous implantation of DuCol showcased time-dependent anti-ageing effects. Early-stage intervention (30 days post-injection) markedly upregulated COL1A1 expression through the TGF-β/Smad3 pathway activation, outperforming poly-<span style="font-variant: small-caps;">l</span>-lactic acid (PLLA) in collagen deposition. Histological analysis revealed 23.4% greater dermal thickness in DuCol-treated groups compared to PLLA at 90 days. While PLLA exhibited sustained collagen stimulation beyond 90 days, DuCol exhibited superior early-phase efficacy (<i>p</i> < 0.001) with comparable safety profiles (no inflammatory response observed through 180-day monitoring). The combinatorial PLLA/DuCol (P&C) formulation synergistically enhanced dermal regeneration, achieving a 31.7% thicker collagen matrix than monotherapy groups. These results underscore the potential of DuCol as a novel implantable filler material for skin repair and regeneration. |
| format | Article |
| id | doaj-art-d3f7d978f427469da9c1a73d7f943a8a |
| institution | Kabale University |
| issn | 2306-5354 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
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| series | Bioengineering |
| spelling | doaj-art-d3f7d978f427469da9c1a73d7f943a8a2025-08-20T03:47:53ZengMDPI AGBioengineering2306-53542025-05-0112551010.3390/bioengineering12050510In Vivo Evaluation of the Anti-Skin-Ageing Bioactivity of a Recombinant Dual Humanised Collagen and Poly-L-Lactic AcidMingjie Tong0Xin Zhou1Jiongni Zhong2Dengjian Qu3Wei Chen4Chun Chen5Yiting Wang6Yaoping Liu7Shaochuan Li8Yuan Xiao9Ning Wang10Chaowan Guo11Qiuling Xie12Sheng Xiong13Institute of Biomedicine and National Engineering Research Center of Genetic Medicine, College of Life Science and Technology, Jinan University, Guangzhou 510632, ChinaInstitute of Biomedicine and National Engineering Research Center of Genetic Medicine, College of Life Science and Technology, Jinan University, Guangzhou 510632, ChinaInstitute of Biomedicine and National Engineering Research Center of Genetic Medicine, College of Life Science and Technology, Jinan University, Guangzhou 510632, ChinaInstitute of Biomedicine and National Engineering Research Center of Genetic Medicine, College of Life Science and Technology, Jinan University, Guangzhou 510632, ChinaGuangzhou Huike Biotech Co., Ltd., Guangzhou 510530, ChinaInstitute of Biomedicine and National Engineering Research Center of Genetic Medicine, College of Life Science and Technology, Jinan University, Guangzhou 510632, ChinaInstitute of Biomedicine and National Engineering Research Center of Genetic Medicine, College of Life Science and Technology, Jinan University, Guangzhou 510632, ChinaGuangzhou Cheer-Derm Biotech Co., Ltd., Guangzhou 510530, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Marubi Biotech Co., Ltd., Guangzhou 510530, ChinaGuangdong Marubi Biotech Co., Ltd., Guangzhou 510530, ChinaGuangdong Marubi Biotech Co., Ltd., Guangzhou 510530, ChinaInstitute of Biomedicine and National Engineering Research Center of Genetic Medicine, College of Life Science and Technology, Jinan University, Guangzhou 510632, ChinaInstitute of Biomedicine and National Engineering Research Center of Genetic Medicine, College of Life Science and Technology, Jinan University, Guangzhou 510632, ChinaThis study introduces a novel recombinant humanised collagen (DuCol) developed through codon optimisation and prokaryotic soluble expression, exhibiting exceptional biocompatibility and bioactivity. Structural integrity was confirmed via RP-HPLC, SEM, and CD spectroscopy. In vitro evaluations revealed DuCol’s dose-dependent enhancement of NIH-3T3 fibroblast proliferation, adhesion, and migration. In a D-galactose-induced ageing rat model, subcutaneous implantation of DuCol showcased time-dependent anti-ageing effects. Early-stage intervention (30 days post-injection) markedly upregulated COL1A1 expression through the TGF-β/Smad3 pathway activation, outperforming poly-<span style="font-variant: small-caps;">l</span>-lactic acid (PLLA) in collagen deposition. Histological analysis revealed 23.4% greater dermal thickness in DuCol-treated groups compared to PLLA at 90 days. While PLLA exhibited sustained collagen stimulation beyond 90 days, DuCol exhibited superior early-phase efficacy (<i>p</i> < 0.001) with comparable safety profiles (no inflammatory response observed through 180-day monitoring). The combinatorial PLLA/DuCol (P&C) formulation synergistically enhanced dermal regeneration, achieving a 31.7% thicker collagen matrix than monotherapy groups. These results underscore the potential of DuCol as a novel implantable filler material for skin repair and regeneration.https://www.mdpi.com/2306-5354/12/5/510recombinant collagenskin ageingTGF-β signallingPLLAinjectable biomaterials |
| spellingShingle | Mingjie Tong Xin Zhou Jiongni Zhong Dengjian Qu Wei Chen Chun Chen Yiting Wang Yaoping Liu Shaochuan Li Yuan Xiao Ning Wang Chaowan Guo Qiuling Xie Sheng Xiong In Vivo Evaluation of the Anti-Skin-Ageing Bioactivity of a Recombinant Dual Humanised Collagen and Poly-L-Lactic Acid Bioengineering recombinant collagen skin ageing TGF-β signalling PLLA injectable biomaterials |
| title | In Vivo Evaluation of the Anti-Skin-Ageing Bioactivity of a Recombinant Dual Humanised Collagen and Poly-L-Lactic Acid |
| title_full | In Vivo Evaluation of the Anti-Skin-Ageing Bioactivity of a Recombinant Dual Humanised Collagen and Poly-L-Lactic Acid |
| title_fullStr | In Vivo Evaluation of the Anti-Skin-Ageing Bioactivity of a Recombinant Dual Humanised Collagen and Poly-L-Lactic Acid |
| title_full_unstemmed | In Vivo Evaluation of the Anti-Skin-Ageing Bioactivity of a Recombinant Dual Humanised Collagen and Poly-L-Lactic Acid |
| title_short | In Vivo Evaluation of the Anti-Skin-Ageing Bioactivity of a Recombinant Dual Humanised Collagen and Poly-L-Lactic Acid |
| title_sort | in vivo evaluation of the anti skin ageing bioactivity of a recombinant dual humanised collagen and poly l lactic acid |
| topic | recombinant collagen skin ageing TGF-β signalling PLLA injectable biomaterials |
| url | https://www.mdpi.com/2306-5354/12/5/510 |
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