The Potency of Maillard Conjugates Containing Whey Protein as Natural Emulsifier

There is a continued need for the advancement of natural emulsifiers to replace synthetic emulsifiers, driven by human health concerns. This study is aimed at producing protein-polysaccharide conjugates through the Maillard reaction and at evaluating its ability as an emulsifier based on its emulsif...

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Main Authors: Iceu Agustinisari, Kamarza Mulia, Niken Harimurti, Mohammad Nasikin, null Rienoviar, Heny Herawati, Lamhot Parulian Manalu
Format: Article
Language:English
Published: Wiley 2024-01-01
Series:International Journal of Food Science
Online Access:http://dx.doi.org/10.1155/2024/3254132
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author Iceu Agustinisari
Kamarza Mulia
Niken Harimurti
Mohammad Nasikin
null Rienoviar
Heny Herawati
Lamhot Parulian Manalu
author_facet Iceu Agustinisari
Kamarza Mulia
Niken Harimurti
Mohammad Nasikin
null Rienoviar
Heny Herawati
Lamhot Parulian Manalu
author_sort Iceu Agustinisari
collection DOAJ
description There is a continued need for the advancement of natural emulsifiers to replace synthetic emulsifiers, driven by human health concerns. This study is aimed at producing protein-polysaccharide conjugates through the Maillard reaction and at evaluating its ability as an emulsifier based on its emulsifying properties. The proteins used in this study were bovine milk whey protein and soy protein isolates, while the polysaccharides were maltodextrin and pectin. The protein-polysaccharide conjugation used a Maillard reaction under dry heating conditions. The protein and polysaccharide mass ratios were 1 : 2 and 1 : 3. The results showed that the types of proteins and polysaccharides and their mass affect the surface tension of the conjugate products. Whey protein-pectin conjugates with a mass ratio of 1 : 2 and a concentration of 1% had the lowest surface tension at 43.77 dyne/cm2. This conjugate sample also showed the highest emulsifying index at 27.20 m2/g. The conjugate powder containing pectin as a polysaccharide showed better emulsifying activity than that of those containing maltodextrin. However, the smallest droplet size of the emulsion (256.5 nm) resulted from the emulsification process using whey protein-maltodextrin conjugates as an emulsifier. The FTIR and gel electrophoresis (SDS-PAGE) analysis confirmed the conjugation formation. In general, protein-polysaccharide conjugates containing whey protein could potentially act as a natural emulsifier for food.
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institution Kabale University
issn 2314-5765
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publishDate 2024-01-01
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series International Journal of Food Science
spelling doaj-art-d3ee7016bc054527afa698eb1120e1342025-02-03T06:15:14ZengWileyInternational Journal of Food Science2314-57652024-01-01202410.1155/2024/3254132The Potency of Maillard Conjugates Containing Whey Protein as Natural EmulsifierIceu Agustinisari0Kamarza Mulia1Niken Harimurti2Mohammad Nasikin3null Rienoviar4Heny Herawati5Lamhot Parulian Manalu6Research Center for AgroindustryDepartment of Chemical EngineeringResearch Center for AgroindustryDepartment of Chemical EngineeringResearch Center for AgroindustryResearch Center for AgroindustryResearch Center for AgroindustryThere is a continued need for the advancement of natural emulsifiers to replace synthetic emulsifiers, driven by human health concerns. This study is aimed at producing protein-polysaccharide conjugates through the Maillard reaction and at evaluating its ability as an emulsifier based on its emulsifying properties. The proteins used in this study were bovine milk whey protein and soy protein isolates, while the polysaccharides were maltodextrin and pectin. The protein-polysaccharide conjugation used a Maillard reaction under dry heating conditions. The protein and polysaccharide mass ratios were 1 : 2 and 1 : 3. The results showed that the types of proteins and polysaccharides and their mass affect the surface tension of the conjugate products. Whey protein-pectin conjugates with a mass ratio of 1 : 2 and a concentration of 1% had the lowest surface tension at 43.77 dyne/cm2. This conjugate sample also showed the highest emulsifying index at 27.20 m2/g. The conjugate powder containing pectin as a polysaccharide showed better emulsifying activity than that of those containing maltodextrin. However, the smallest droplet size of the emulsion (256.5 nm) resulted from the emulsification process using whey protein-maltodextrin conjugates as an emulsifier. The FTIR and gel electrophoresis (SDS-PAGE) analysis confirmed the conjugation formation. In general, protein-polysaccharide conjugates containing whey protein could potentially act as a natural emulsifier for food.http://dx.doi.org/10.1155/2024/3254132
spellingShingle Iceu Agustinisari
Kamarza Mulia
Niken Harimurti
Mohammad Nasikin
null Rienoviar
Heny Herawati
Lamhot Parulian Manalu
The Potency of Maillard Conjugates Containing Whey Protein as Natural Emulsifier
International Journal of Food Science
title The Potency of Maillard Conjugates Containing Whey Protein as Natural Emulsifier
title_full The Potency of Maillard Conjugates Containing Whey Protein as Natural Emulsifier
title_fullStr The Potency of Maillard Conjugates Containing Whey Protein as Natural Emulsifier
title_full_unstemmed The Potency of Maillard Conjugates Containing Whey Protein as Natural Emulsifier
title_short The Potency of Maillard Conjugates Containing Whey Protein as Natural Emulsifier
title_sort potency of maillard conjugates containing whey protein as natural emulsifier
url http://dx.doi.org/10.1155/2024/3254132
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