Microscopic Observation of Proliferative Membranes in Fibrocontractive Retinal Disorders
Proliferative membranes of fibrocontractive retinal disorders are extensively studied from the morphological and evolutive point of view. Despite this, little is known of their cellular composition. In this study, the authors investigated the morphological characteristics and cell composition of var...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-01-01
|
| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2019/9647947 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832560972101844992 |
|---|---|
| author | Rino Frisina Francesco Tessarolo Ivan Marchesoni Federico Piccoli Emiliana Bonomi Patrizio Caciagli Edoardo Midena Giandomenico Nollo |
| author_facet | Rino Frisina Francesco Tessarolo Ivan Marchesoni Federico Piccoli Emiliana Bonomi Patrizio Caciagli Edoardo Midena Giandomenico Nollo |
| author_sort | Rino Frisina |
| collection | DOAJ |
| description | Proliferative membranes of fibrocontractive retinal disorders are extensively studied from the morphological and evolutive point of view. Despite this, little is known of their cellular composition. In this study, the authors investigated the morphological characteristics and cell composition of various types of surgically excised proliferative membranes and internal limiting membranes (ILMs), in order to provide new data supporting or challenging the pathogenic theories proposed until now. Sixty-nine specimens from 64 eyes of 64 consecutive patients were collected at surgery and subjected to a multilevel analysis by means of optical and electron microscopy. Membrane samples were semiquantitatively evaluated for the amount and distribution of cell nuclei and pigment. Immunohistochemical staining was performed with antibodies to alpha smooth muscle actin and CD68. Data were analyzed after grouping according to the following tissue types: ILM (20 specimens), epiretinal membrane (ERM) (22 specimens), ILM + ERM (20 specimens), and proliferative vitreoretinopathy (PVR) (7 specimens). The cell components found in the ERM specimens, like myofibroblasts, macrophages, and polymorphonuclear cells, were recognized as the expression of cell migration and differentiation that induced an inflammatory process and a fibroproliferative repair process. The detection of pigments in specific types of ERM, like those associated with lamellar macular hole (LMH) or secondary to retinal detachment (RD), diabetes, and PVR, suggested that retinal pigment epithelium (RPE) cells may have a role in the development of these vitreoretinal disorders. The reduction of the ERM cellularity with the patient’s age supports the hypothesis that ERM evolves in time up to a fibrous tissue formation. |
| format | Article |
| id | doaj-art-d3e37906ce544a20bcab9a91caf76807 |
| institution | Kabale University |
| issn | 2090-004X 2090-0058 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-d3e37906ce544a20bcab9a91caf768072025-02-03T01:26:22ZengWileyJournal of Ophthalmology2090-004X2090-00582019-01-01201910.1155/2019/96479479647947Microscopic Observation of Proliferative Membranes in Fibrocontractive Retinal DisordersRino Frisina0Francesco Tessarolo1Ivan Marchesoni2Federico Piccoli3Emiliana Bonomi4Patrizio Caciagli5Edoardo Midena6Giandomenico Nollo7Department of Ophthalmology, University of Padova, Via Giustiniani 2, I-35128 Padova, ItalyDepartment of Industrial Engineering, University of Trento, Via Delle Regole 101, I-38123 Mattarello, Trento, ItalyMultizone Unit of Ophthalmology of Autonomous Province of Trento, Corso Verona 6, I-38123 Rovereto, Trento, ItalyDepartment of Laboratory Medicine, Azienda Provinciale per i Servizi Sanitari, Largo Medaglie d’Oro 9, Trento, ItalyHealthcare Research and Innovation Program (IRCS-PAT), Bruno Kessler Foundation, Via Sommarive 18, I-38123 Trento, ItalyDepartment of Laboratory Medicine, Azienda Provinciale per i Servizi Sanitari, Largo Medaglie d’Oro 9, Trento, ItalyDepartment of Ophthalmology, University of Padova, Via Giustiniani 2, I-35128 Padova, ItalyDepartment of Industrial Engineering, University of Trento, Via Delle Regole 101, I-38123 Mattarello, Trento, ItalyProliferative membranes of fibrocontractive retinal disorders are extensively studied from the morphological and evolutive point of view. Despite this, little is known of their cellular composition. In this study, the authors investigated the morphological characteristics and cell composition of various types of surgically excised proliferative membranes and internal limiting membranes (ILMs), in order to provide new data supporting or challenging the pathogenic theories proposed until now. Sixty-nine specimens from 64 eyes of 64 consecutive patients were collected at surgery and subjected to a multilevel analysis by means of optical and electron microscopy. Membrane samples were semiquantitatively evaluated for the amount and distribution of cell nuclei and pigment. Immunohistochemical staining was performed with antibodies to alpha smooth muscle actin and CD68. Data were analyzed after grouping according to the following tissue types: ILM (20 specimens), epiretinal membrane (ERM) (22 specimens), ILM + ERM (20 specimens), and proliferative vitreoretinopathy (PVR) (7 specimens). The cell components found in the ERM specimens, like myofibroblasts, macrophages, and polymorphonuclear cells, were recognized as the expression of cell migration and differentiation that induced an inflammatory process and a fibroproliferative repair process. The detection of pigments in specific types of ERM, like those associated with lamellar macular hole (LMH) or secondary to retinal detachment (RD), diabetes, and PVR, suggested that retinal pigment epithelium (RPE) cells may have a role in the development of these vitreoretinal disorders. The reduction of the ERM cellularity with the patient’s age supports the hypothesis that ERM evolves in time up to a fibrous tissue formation.http://dx.doi.org/10.1155/2019/9647947 |
| spellingShingle | Rino Frisina Francesco Tessarolo Ivan Marchesoni Federico Piccoli Emiliana Bonomi Patrizio Caciagli Edoardo Midena Giandomenico Nollo Microscopic Observation of Proliferative Membranes in Fibrocontractive Retinal Disorders Journal of Ophthalmology |
| title | Microscopic Observation of Proliferative Membranes in Fibrocontractive Retinal Disorders |
| title_full | Microscopic Observation of Proliferative Membranes in Fibrocontractive Retinal Disorders |
| title_fullStr | Microscopic Observation of Proliferative Membranes in Fibrocontractive Retinal Disorders |
| title_full_unstemmed | Microscopic Observation of Proliferative Membranes in Fibrocontractive Retinal Disorders |
| title_short | Microscopic Observation of Proliferative Membranes in Fibrocontractive Retinal Disorders |
| title_sort | microscopic observation of proliferative membranes in fibrocontractive retinal disorders |
| url | http://dx.doi.org/10.1155/2019/9647947 |
| work_keys_str_mv | AT rinofrisina microscopicobservationofproliferativemembranesinfibrocontractiveretinaldisorders AT francescotessarolo microscopicobservationofproliferativemembranesinfibrocontractiveretinaldisorders AT ivanmarchesoni microscopicobservationofproliferativemembranesinfibrocontractiveretinaldisorders AT federicopiccoli microscopicobservationofproliferativemembranesinfibrocontractiveretinaldisorders AT emilianabonomi microscopicobservationofproliferativemembranesinfibrocontractiveretinaldisorders AT patriziocaciagli microscopicobservationofproliferativemembranesinfibrocontractiveretinaldisorders AT edoardomidena microscopicobservationofproliferativemembranesinfibrocontractiveretinaldisorders AT giandomeniconollo microscopicobservationofproliferativemembranesinfibrocontractiveretinaldisorders |