Genome-Wide Analysis of C2H2 Zinc Finger Gene Family and Exploration of Genes Related to Prickle Development in <i>Zanthoxylum armatum</i>
<i>Zanthoxylum armatum</i> (<i>Z. armatum</i>) is a significant economic tree species known for its medicinal and edible properties. However, the presence of prickles on <i>Z. armatum</i> poses a considerable challenge to the advancement of its industry. Numerous...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
|
| Series: | Horticulturae |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-7524/10/12/1371 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850050847816286208 |
|---|---|
| author | Nuo Wang Renhua Huang Hualin Zou Yuwei Yi Jiabao Ye Weiwei Zhang Feng Xu Yongling Liao |
| author_facet | Nuo Wang Renhua Huang Hualin Zou Yuwei Yi Jiabao Ye Weiwei Zhang Feng Xu Yongling Liao |
| author_sort | Nuo Wang |
| collection | DOAJ |
| description | <i>Zanthoxylum armatum</i> (<i>Z. armatum</i>) is a significant economic tree species known for its medicinal and edible properties. However, the presence of prickles on <i>Z. armatum</i> poses a considerable challenge to the advancement of its industry. Numerous studies have indicated that the C2H2 zinc finger protein (C2H2-ZFPs) families are crucial in the development of plant trichomes or prickles. This study identified 78 <i>ZaC2H2</i> genes from the <i>Z. armatum</i> genome, categorizing them into three groups and analyzing their protein physicochemical properties, chromosomal locations, conserved domains, and gene structures. The evolutionary analysis indicates that the amplification of <i>ZaC2H2</i> genes primarily results from whole-genome duplication or segmental duplication, and these genes have undergone strong purifying selection pressure throughout their evolutionary history. The analysis of <i>cis</i>-acting elements revealed that they contain various hormone response elements, such as ABRE, AuxRR, the CGTCA motif, GARE motifs, and TCA elements, which are responsive to ABA, IAA, MeJA, GA, and SA signals. RT-qPCR was employed to assess the expression levels of the candidate genes <i>ZaC2H2-45</i>, <i>ZaC2H2-46</i>, <i>ZaC2H2-49,</i> and <i>ZaC2H2-55</i> under the treatment of five hormones. The results indicated that the expression levels of the <i>ZaC2H2-46</i> and <i>ZaC2H2-55</i> genes were significantly up-regulated under NAA, SA, and MeJA treatments. These results will help to further understand the characteristics of the <i>ZaC2H2</i> gene family and provide a theoretical basis for studying the development of prickles. |
| format | Article |
| id | doaj-art-d3e1d4d827864422b3c6b7bb742b2e4a |
| institution | DOAJ |
| issn | 2311-7524 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj-art-d3e1d4d827864422b3c6b7bb742b2e4a2025-08-20T02:53:19ZengMDPI AGHorticulturae2311-75242024-12-011012137110.3390/horticulturae10121371Genome-Wide Analysis of C2H2 Zinc Finger Gene Family and Exploration of Genes Related to Prickle Development in <i>Zanthoxylum armatum</i>Nuo Wang0Renhua Huang1Hualin Zou2Yuwei Yi3Jiabao Ye4Weiwei Zhang5Feng Xu6Yongling Liao7College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, ChinaCollege of Food and Biology, Jingchu University of Technology, Jingmen 448000, ChinaCollege of Horticulture and Gardening, Yangtze University, Jingzhou 434025, ChinaCollege of Horticulture and Gardening, Yangtze University, Jingzhou 434025, ChinaCollege of Horticulture and Gardening, Yangtze University, Jingzhou 434025, ChinaCollege of Horticulture and Gardening, Yangtze University, Jingzhou 434025, ChinaCollege of Horticulture and Gardening, Yangtze University, Jingzhou 434025, ChinaCollege of Horticulture and Gardening, Yangtze University, Jingzhou 434025, China<i>Zanthoxylum armatum</i> (<i>Z. armatum</i>) is a significant economic tree species known for its medicinal and edible properties. However, the presence of prickles on <i>Z. armatum</i> poses a considerable challenge to the advancement of its industry. Numerous studies have indicated that the C2H2 zinc finger protein (C2H2-ZFPs) families are crucial in the development of plant trichomes or prickles. This study identified 78 <i>ZaC2H2</i> genes from the <i>Z. armatum</i> genome, categorizing them into three groups and analyzing their protein physicochemical properties, chromosomal locations, conserved domains, and gene structures. The evolutionary analysis indicates that the amplification of <i>ZaC2H2</i> genes primarily results from whole-genome duplication or segmental duplication, and these genes have undergone strong purifying selection pressure throughout their evolutionary history. The analysis of <i>cis</i>-acting elements revealed that they contain various hormone response elements, such as ABRE, AuxRR, the CGTCA motif, GARE motifs, and TCA elements, which are responsive to ABA, IAA, MeJA, GA, and SA signals. RT-qPCR was employed to assess the expression levels of the candidate genes <i>ZaC2H2-45</i>, <i>ZaC2H2-46</i>, <i>ZaC2H2-49,</i> and <i>ZaC2H2-55</i> under the treatment of five hormones. The results indicated that the expression levels of the <i>ZaC2H2-46</i> and <i>ZaC2H2-55</i> genes were significantly up-regulated under NAA, SA, and MeJA treatments. These results will help to further understand the characteristics of the <i>ZaC2H2</i> gene family and provide a theoretical basis for studying the development of prickles.https://www.mdpi.com/2311-7524/10/12/1371C2H2zinc finger proteinsgene familycollinearity analysisRT-qPCR |
| spellingShingle | Nuo Wang Renhua Huang Hualin Zou Yuwei Yi Jiabao Ye Weiwei Zhang Feng Xu Yongling Liao Genome-Wide Analysis of C2H2 Zinc Finger Gene Family and Exploration of Genes Related to Prickle Development in <i>Zanthoxylum armatum</i> Horticulturae C2H2 zinc finger proteins gene family collinearity analysis RT-qPCR |
| title | Genome-Wide Analysis of C2H2 Zinc Finger Gene Family and Exploration of Genes Related to Prickle Development in <i>Zanthoxylum armatum</i> |
| title_full | Genome-Wide Analysis of C2H2 Zinc Finger Gene Family and Exploration of Genes Related to Prickle Development in <i>Zanthoxylum armatum</i> |
| title_fullStr | Genome-Wide Analysis of C2H2 Zinc Finger Gene Family and Exploration of Genes Related to Prickle Development in <i>Zanthoxylum armatum</i> |
| title_full_unstemmed | Genome-Wide Analysis of C2H2 Zinc Finger Gene Family and Exploration of Genes Related to Prickle Development in <i>Zanthoxylum armatum</i> |
| title_short | Genome-Wide Analysis of C2H2 Zinc Finger Gene Family and Exploration of Genes Related to Prickle Development in <i>Zanthoxylum armatum</i> |
| title_sort | genome wide analysis of c2h2 zinc finger gene family and exploration of genes related to prickle development in i zanthoxylum armatum i |
| topic | C2H2 zinc finger proteins gene family collinearity analysis RT-qPCR |
| url | https://www.mdpi.com/2311-7524/10/12/1371 |
| work_keys_str_mv | AT nuowang genomewideanalysisofc2h2zincfingergenefamilyandexplorationofgenesrelatedtoprickledevelopmentinizanthoxylumarmatumi AT renhuahuang genomewideanalysisofc2h2zincfingergenefamilyandexplorationofgenesrelatedtoprickledevelopmentinizanthoxylumarmatumi AT hualinzou genomewideanalysisofc2h2zincfingergenefamilyandexplorationofgenesrelatedtoprickledevelopmentinizanthoxylumarmatumi AT yuweiyi genomewideanalysisofc2h2zincfingergenefamilyandexplorationofgenesrelatedtoprickledevelopmentinizanthoxylumarmatumi AT jiabaoye genomewideanalysisofc2h2zincfingergenefamilyandexplorationofgenesrelatedtoprickledevelopmentinizanthoxylumarmatumi AT weiweizhang genomewideanalysisofc2h2zincfingergenefamilyandexplorationofgenesrelatedtoprickledevelopmentinizanthoxylumarmatumi AT fengxu genomewideanalysisofc2h2zincfingergenefamilyandexplorationofgenesrelatedtoprickledevelopmentinizanthoxylumarmatumi AT yonglingliao genomewideanalysisofc2h2zincfingergenefamilyandexplorationofgenesrelatedtoprickledevelopmentinizanthoxylumarmatumi |