Seroprevalence of Bordetella pertussis antibodies in Libyan pregnant women at the time of delivery

Background: Pertussis is a highly contagious respiratory disease. Low levels of maternal antibodies make infants more susceptible to pertussis infection before they are eligible for vaccination at 2 months of age. This study aimed to investigate the seroprevalence of antibodies against Bordetella pe...

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Main Authors: Suleiman Abusrewil, Mostafa Gawass, Inas Alhudiri, Marwan Sherif, Fawzi Ebrahim, Ali Amgadmi, Yosra Lamami, Salah Tabal, Musa O. Busarira, Hussain Asherkaci, Lubna Almaghur, Samira Al Dwigen, Mohamed Milad, Fatma Faraj Alhoty, Fatimah Abouthiheer, Zaynab Algharyani, Mirfat Albasheer, Hana Elghoul, Shaima Abuzaid, Khoulod Tawel, Weam Ben Hamza, Adam Elzagheid
Format: Article
Language:English
Published: Elsevier 2025-08-01
Series:Vaccine: X
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Online Access:http://www.sciencedirect.com/science/article/pii/S2590136225000890
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author Suleiman Abusrewil
Mostafa Gawass
Inas Alhudiri
Marwan Sherif
Fawzi Ebrahim
Ali Amgadmi
Yosra Lamami
Salah Tabal
Musa O. Busarira
Hussain Asherkaci
Lubna Almaghur
Samira Al Dwigen
Mohamed Milad
Fatma Faraj Alhoty
Fatimah Abouthiheer
Zaynab Algharyani
Mirfat Albasheer
Hana Elghoul
Shaima Abuzaid
Khoulod Tawel
Weam Ben Hamza
Adam Elzagheid
author_facet Suleiman Abusrewil
Mostafa Gawass
Inas Alhudiri
Marwan Sherif
Fawzi Ebrahim
Ali Amgadmi
Yosra Lamami
Salah Tabal
Musa O. Busarira
Hussain Asherkaci
Lubna Almaghur
Samira Al Dwigen
Mohamed Milad
Fatma Faraj Alhoty
Fatimah Abouthiheer
Zaynab Algharyani
Mirfat Albasheer
Hana Elghoul
Shaima Abuzaid
Khoulod Tawel
Weam Ben Hamza
Adam Elzagheid
author_sort Suleiman Abusrewil
collection DOAJ
description Background: Pertussis is a highly contagious respiratory disease. Low levels of maternal antibodies make infants more susceptible to pertussis infection before they are eligible for vaccination at 2 months of age. This study aimed to investigate the seroprevalence of antibodies against Bordetella pertussis in Libyan pregnant women at the time of delivery. Methods: In this observational cross-sectional clinic-based study, 840 pregnant women were enrolled at five tertiary hospitals in three major cities in Libya from March 2023 to February 2024. Informed consent was obtained from all subjects involved in the study. At the time of delivery, venous blood was extracted from participants, and the samples were analyzed for serum levels of anti-B.pertussis toxin antibodies (IgG and IgA) using enzyme-linked immunosorbent assay (ELISA). Samples were positive if the antibody level was ≥100 IU/mL for IgG or ≥ 12 IU/mL for IgA. Results: Mean age of pregnant women was 31.1 ± 6.2 years old. All participants had received four doses of whole cell pertussis vaccine as part of the expanded Libyan national immunization program during their childhood. In this study, 827 (98.5 %) participants had IgG and IgA antibodies to B.pertussis toxin below the cut-off level of the assay. Only 13 (1.5 %) participants had recent infections (Two were positive for IgG, nine were positive for IgA antibodies and two were positive for both antibodies). Conclusions: In this study, most pregnant women in Libya were seronegative for B. pertussis antibodies. This makes their young infants more vulnerable to pertussis infection at this critical age. These findings support the need to introduce maternal Tdap vaccination as an important preventive strategy to reduce the burden of neonatal and younger infants' pertussis infections in Libya. National immunization programs should prioritize maternal vaccination, booster doses, and enhanced surveillance to protect the most vulnerable groups.
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spelling doaj-art-d3d8a250f4b642b1ba92d1be1b830a4e2025-08-20T04:01:57ZengElsevierVaccine: X2590-13622025-08-012510069510.1016/j.jvacx.2025.100695Seroprevalence of Bordetella pertussis antibodies in Libyan pregnant women at the time of deliverySuleiman Abusrewil0Mostafa Gawass1Inas Alhudiri2Marwan Sherif3Fawzi Ebrahim4Ali Amgadmi5Yosra Lamami6Salah Tabal7Musa O. Busarira8Hussain Asherkaci9Lubna Almaghur10Samira Al Dwigen11Mohamed Milad12Fatma Faraj Alhoty13Fatimah Abouthiheer14Zaynab Algharyani15Mirfat Albasheer16Hana Elghoul17Shaima Abuzaid18Khoulod Tawel19Weam Ben Hamza20Adam Elzagheid21Tripoli University Hospital, Tripoli, Libya; University of Tripoli, Tripoli, Libya; Libyan Board of Medical Specialties, Tripoli, Libya; Corresponding author : Prof. Suleiman Abusrewil, Department of Paediatrics, Tripoli University Hospital, Tripoli, Libya.Tripoli University Hospital, Tripoli, Libya; University of Tripoli, Tripoli, LibyaLibyan Biotechnology Research Center, Tripoli, LibyaLibyan Board of Medical Specialties, Tripoli, Libya; Libyan Biotechnology Research Center, Tripoli, LibyaLibyan Biotechnology Research Center, Tripoli, LibyaTripoli University Hospital, Tripoli, Libya; University of Tripoli, Tripoli, LibyaLibyan Biotechnology Research Center, Tripoli, LibyaLibyan Biotechnology Research Center, Tripoli, LibyaUniversity of Benghazi, Benghazi, Libya; Benghazi Medical Center, Benghazi, LibyaMisrata Medical Center, Misrata, LibyaTripoli University Hospital, Tripoli, LibyaLibyan Biotechnology Research Center, Tripoli, LibyaLibyan Biotechnology Research Center, Tripoli, LibyaUniversity of Benghazi, Benghazi, Libya; Benghazi Medical Center, Benghazi, LibyaUniversity of Benghazi, Benghazi, Libya; Benghazi Medical Center, Benghazi, LibyaUniversity of Benghazi, Benghazi, Libya; Benghazi Medical Center, Benghazi, LibyaAl Khadra Hospital, Tripoli, LibyaTripoli University Hospital, Tripoli, LibyaAjalaa Maternity Hospital, Tripoli, LibyaAjalaa Maternity Hospital, Tripoli, LibyaTripoli University Hospital, Tripoli, LibyaLibyan Biotechnology Research Center, Tripoli, LibyaBackground: Pertussis is a highly contagious respiratory disease. Low levels of maternal antibodies make infants more susceptible to pertussis infection before they are eligible for vaccination at 2 months of age. This study aimed to investigate the seroprevalence of antibodies against Bordetella pertussis in Libyan pregnant women at the time of delivery. Methods: In this observational cross-sectional clinic-based study, 840 pregnant women were enrolled at five tertiary hospitals in three major cities in Libya from March 2023 to February 2024. Informed consent was obtained from all subjects involved in the study. At the time of delivery, venous blood was extracted from participants, and the samples were analyzed for serum levels of anti-B.pertussis toxin antibodies (IgG and IgA) using enzyme-linked immunosorbent assay (ELISA). Samples were positive if the antibody level was ≥100 IU/mL for IgG or ≥ 12 IU/mL for IgA. Results: Mean age of pregnant women was 31.1 ± 6.2 years old. All participants had received four doses of whole cell pertussis vaccine as part of the expanded Libyan national immunization program during their childhood. In this study, 827 (98.5 %) participants had IgG and IgA antibodies to B.pertussis toxin below the cut-off level of the assay. Only 13 (1.5 %) participants had recent infections (Two were positive for IgG, nine were positive for IgA antibodies and two were positive for both antibodies). Conclusions: In this study, most pregnant women in Libya were seronegative for B. pertussis antibodies. This makes their young infants more vulnerable to pertussis infection at this critical age. These findings support the need to introduce maternal Tdap vaccination as an important preventive strategy to reduce the burden of neonatal and younger infants' pertussis infections in Libya. National immunization programs should prioritize maternal vaccination, booster doses, and enhanced surveillance to protect the most vulnerable groups.http://www.sciencedirect.com/science/article/pii/S2590136225000890Whooping coughMaternal vaccinationImmune response
spellingShingle Suleiman Abusrewil
Mostafa Gawass
Inas Alhudiri
Marwan Sherif
Fawzi Ebrahim
Ali Amgadmi
Yosra Lamami
Salah Tabal
Musa O. Busarira
Hussain Asherkaci
Lubna Almaghur
Samira Al Dwigen
Mohamed Milad
Fatma Faraj Alhoty
Fatimah Abouthiheer
Zaynab Algharyani
Mirfat Albasheer
Hana Elghoul
Shaima Abuzaid
Khoulod Tawel
Weam Ben Hamza
Adam Elzagheid
Seroprevalence of Bordetella pertussis antibodies in Libyan pregnant women at the time of delivery
Vaccine: X
Whooping cough
Maternal vaccination
Immune response
title Seroprevalence of Bordetella pertussis antibodies in Libyan pregnant women at the time of delivery
title_full Seroprevalence of Bordetella pertussis antibodies in Libyan pregnant women at the time of delivery
title_fullStr Seroprevalence of Bordetella pertussis antibodies in Libyan pregnant women at the time of delivery
title_full_unstemmed Seroprevalence of Bordetella pertussis antibodies in Libyan pregnant women at the time of delivery
title_short Seroprevalence of Bordetella pertussis antibodies in Libyan pregnant women at the time of delivery
title_sort seroprevalence of bordetella pertussis antibodies in libyan pregnant women at the time of delivery
topic Whooping cough
Maternal vaccination
Immune response
url http://www.sciencedirect.com/science/article/pii/S2590136225000890
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