Measurement of Phenolic Compounds by Spectrometry and Chromatography in Lime Juices

Juice demand has been increasing at a rapid rate in recent years, and one of the major efforts underway to meet this demand is to minimize changes during the juice process. Due to the high consumption of juice and the carcinogenicity of synthetic and chemical substances, this research uses high perf...

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Main Authors: Fatemeh Shakeri, Mohammad Shakeri, Fatemeh Pour Ramezani, Mohadeseh Pirhadi, Masoud Aman Mohammadi, Samira Shokri, Tayebeh Zeinali
Format: Article
Language:English
Published: Wiley 2022-01-01
Series:Journal of Spectroscopy
Online Access:http://dx.doi.org/10.1155/2022/9228394
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author Fatemeh Shakeri
Mohammad Shakeri
Fatemeh Pour Ramezani
Mohadeseh Pirhadi
Masoud Aman Mohammadi
Samira Shokri
Tayebeh Zeinali
author_facet Fatemeh Shakeri
Mohammad Shakeri
Fatemeh Pour Ramezani
Mohadeseh Pirhadi
Masoud Aman Mohammadi
Samira Shokri
Tayebeh Zeinali
author_sort Fatemeh Shakeri
collection DOAJ
description Juice demand has been increasing at a rapid rate in recent years, and one of the major efforts underway to meet this demand is to minimize changes during the juice process. Due to the high consumption of juice and the carcinogenicity of synthetic and chemical substances, this research uses high performance liquid chromatography (HPLC) and spectrophotometry to detect fake juices. For the detection of fraud, the tests of sodium and potassium content along with determining the amount of flavonoids (hesperidin and eriocitrin) were carried out using spectrophotometry and HPLC. The results showed the average amount of total polyphenol was from 32.4 to 42.6 mg L-1. The total polyphenol content in all samples conformed to the standard, and there was no significant difference between the samples and the standard. The amounts of flavonoids (hesperidin and eriocitrin) in the juice samples were below the standard level (a minimum of 90 and 20 g/mL, respectively). Also, there was a significant difference between the mean sodium and potassium content of standard versus feigned juices. Generally, the amount of hesperidin, eriocitrin, Na+, and K+ as diagnostic biomarkers of natural juice in all samples was below the standard level. All the analysed samples in the experiment were nonstandard. There is a lot of fraud in the juice business, so it has been suggested that the government should have more control over how manufacturing companies make juice.
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institution Kabale University
issn 2314-4939
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publishDate 2022-01-01
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series Journal of Spectroscopy
spelling doaj-art-d3d1f121262b4be3b64745c5a68a17742025-08-20T03:24:14ZengWileyJournal of Spectroscopy2314-49392022-01-01202210.1155/2022/9228394Measurement of Phenolic Compounds by Spectrometry and Chromatography in Lime JuicesFatemeh Shakeri0Mohammad Shakeri1Fatemeh Pour Ramezani2Mohadeseh Pirhadi3Masoud Aman Mohammadi4Samira Shokri5Tayebeh Zeinali6Food Health Research CenterDepartment of Food Science and TechnologyFood Hygiene and SafetyDepartment of Environmental Health EngineeringStudent Research CommitteeDepartment of Environmental Health EngineeringDepartment of Public HealthJuice demand has been increasing at a rapid rate in recent years, and one of the major efforts underway to meet this demand is to minimize changes during the juice process. Due to the high consumption of juice and the carcinogenicity of synthetic and chemical substances, this research uses high performance liquid chromatography (HPLC) and spectrophotometry to detect fake juices. For the detection of fraud, the tests of sodium and potassium content along with determining the amount of flavonoids (hesperidin and eriocitrin) were carried out using spectrophotometry and HPLC. The results showed the average amount of total polyphenol was from 32.4 to 42.6 mg L-1. The total polyphenol content in all samples conformed to the standard, and there was no significant difference between the samples and the standard. The amounts of flavonoids (hesperidin and eriocitrin) in the juice samples were below the standard level (a minimum of 90 and 20 g/mL, respectively). Also, there was a significant difference between the mean sodium and potassium content of standard versus feigned juices. Generally, the amount of hesperidin, eriocitrin, Na+, and K+ as diagnostic biomarkers of natural juice in all samples was below the standard level. All the analysed samples in the experiment were nonstandard. There is a lot of fraud in the juice business, so it has been suggested that the government should have more control over how manufacturing companies make juice.http://dx.doi.org/10.1155/2022/9228394
spellingShingle Fatemeh Shakeri
Mohammad Shakeri
Fatemeh Pour Ramezani
Mohadeseh Pirhadi
Masoud Aman Mohammadi
Samira Shokri
Tayebeh Zeinali
Measurement of Phenolic Compounds by Spectrometry and Chromatography in Lime Juices
Journal of Spectroscopy
title Measurement of Phenolic Compounds by Spectrometry and Chromatography in Lime Juices
title_full Measurement of Phenolic Compounds by Spectrometry and Chromatography in Lime Juices
title_fullStr Measurement of Phenolic Compounds by Spectrometry and Chromatography in Lime Juices
title_full_unstemmed Measurement of Phenolic Compounds by Spectrometry and Chromatography in Lime Juices
title_short Measurement of Phenolic Compounds by Spectrometry and Chromatography in Lime Juices
title_sort measurement of phenolic compounds by spectrometry and chromatography in lime juices
url http://dx.doi.org/10.1155/2022/9228394
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