New insights into the mechanisms of iron absorption: Iron dextran uptake in the intestines of weaned pigs through glucose transporter 5 (GLUT5) and divalent metal transporter 1 (DMT1) transporters
The purpose of this study was to gain insight into the mechanism of iron dextran (DexFe) absorption in the intestines. A total of 72 piglets (average BW = 7.12 ± 0.75 kg, male to female ratio = 1:1) weaned at 28 d of age were randomly divided into two treatment groups with six replicates for each gr...
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KeAi Communications Co., Ltd.
2024-12-01
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| Series: | Animal Nutrition |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405654524001008 |
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| author | Shengting Deng Weiguang Yang Chengkun Fang Haosheng He Jiamin Liu Rejun Fang |
| author_facet | Shengting Deng Weiguang Yang Chengkun Fang Haosheng He Jiamin Liu Rejun Fang |
| author_sort | Shengting Deng |
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| description | The purpose of this study was to gain insight into the mechanism of iron dextran (DexFe) absorption in the intestines. A total of 72 piglets (average BW = 7.12 ± 0.75 kg, male to female ratio = 1:1) weaned at 28 d of age were randomly divided into two treatment groups with six replicates for each group. The experimental diets included the basal diet supplemented with 100 mg/kg iron dextran (DexFe group) and the basal diet supplemented with 100 mg/kg FeSO4·H2O (CON group). The experiment lasted for 28 d. The piglets' intestinal iron transport was measured in vitro using an Ussing chamber. Porcine intestinal epithelial cell line (IPEC-J2) cells were used to develop a monolayer cell model that explored the molecular mechanism of DexFe absorption. Results showed that compared to the CON group, the ADG of pigs in the DexFe group was improved (P = 0.022), while the F/G was decreased (P = 0.015). The serum iron concentration, apparent iron digestibility, and iron deposition in the duodenum, jejunum, and ileum were increased (P < 0.05) by dietary DexFe supplementation. Piglets in the DexFe group had higher serum red blood count, hemoglobin, serum iron content, serum ferritin and transferrin levels and lower total iron binding capacity (P < 0.05). In the Ussing chamber test, the iron absorption rate of the DexFe group was greater (P < 0.001) than the CON group, and there was no significant difference between the DexFe group and the glucose group (P > 0.05). Furthermore, when compared to the CON group, DexFe administration improved (P < 0.05) SLC2A5 gene and glucose transporter 5 (GLUT5) protein expression but had no effect (P > 0.05) on SLC11A2 gene or divalent metal transporter 1 (DMT1) protein expression. Once the GLUT5 protein was suppressed, the iron transport rate and apparent permeability coefficient were decreased (P < 0.05) in IPEC-J2 monolayer cell models. The findings suggest the effectiveness of DexFe application in weaned piglets and revealed for the first time that DexFe absorption in the intestine is closely related to the glucose transporter GLUT5 protein channel. |
| format | Article |
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| institution | DOAJ |
| issn | 2405-6545 |
| language | English |
| publishDate | 2024-12-01 |
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| spelling | doaj-art-d3ce1d4701014cfbac5fe7f9ff8157732025-08-20T02:51:19ZengKeAi Communications Co., Ltd.Animal Nutrition2405-65452024-12-0119254010.1016/j.aninu.2024.05.006New insights into the mechanisms of iron absorption: Iron dextran uptake in the intestines of weaned pigs through glucose transporter 5 (GLUT5) and divalent metal transporter 1 (DMT1) transportersShengting Deng0Weiguang Yang1Chengkun Fang2Haosheng He3Jiamin Liu4Rejun Fang5College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China; Hunan Engineering Research Center of Intelligent Animal Husbandry, Changsha 410128, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China; Hunan Engineering Research Center of Intelligent Animal Husbandry, Changsha 410128, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China; Hunan Engineering Research Center of Intelligent Animal Husbandry, Changsha 410128, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China; Hunan Engineering Research Center of Intelligent Animal Husbandry, Changsha 410128, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China; Hunan Engineering Research Center of Intelligent Animal Husbandry, Changsha 410128, China; Corresponding author.The purpose of this study was to gain insight into the mechanism of iron dextran (DexFe) absorption in the intestines. A total of 72 piglets (average BW = 7.12 ± 0.75 kg, male to female ratio = 1:1) weaned at 28 d of age were randomly divided into two treatment groups with six replicates for each group. The experimental diets included the basal diet supplemented with 100 mg/kg iron dextran (DexFe group) and the basal diet supplemented with 100 mg/kg FeSO4·H2O (CON group). The experiment lasted for 28 d. The piglets' intestinal iron transport was measured in vitro using an Ussing chamber. Porcine intestinal epithelial cell line (IPEC-J2) cells were used to develop a monolayer cell model that explored the molecular mechanism of DexFe absorption. Results showed that compared to the CON group, the ADG of pigs in the DexFe group was improved (P = 0.022), while the F/G was decreased (P = 0.015). The serum iron concentration, apparent iron digestibility, and iron deposition in the duodenum, jejunum, and ileum were increased (P < 0.05) by dietary DexFe supplementation. Piglets in the DexFe group had higher serum red blood count, hemoglobin, serum iron content, serum ferritin and transferrin levels and lower total iron binding capacity (P < 0.05). In the Ussing chamber test, the iron absorption rate of the DexFe group was greater (P < 0.001) than the CON group, and there was no significant difference between the DexFe group and the glucose group (P > 0.05). Furthermore, when compared to the CON group, DexFe administration improved (P < 0.05) SLC2A5 gene and glucose transporter 5 (GLUT5) protein expression but had no effect (P > 0.05) on SLC11A2 gene or divalent metal transporter 1 (DMT1) protein expression. Once the GLUT5 protein was suppressed, the iron transport rate and apparent permeability coefficient were decreased (P < 0.05) in IPEC-J2 monolayer cell models. The findings suggest the effectiveness of DexFe application in weaned piglets and revealed for the first time that DexFe absorption in the intestine is closely related to the glucose transporter GLUT5 protein channel.http://www.sciencedirect.com/science/article/pii/S2405654524001008Iron dextranWeaned pigletDivalent metal transporter 1Glucose transporter 5Intestinal iron absorption |
| spellingShingle | Shengting Deng Weiguang Yang Chengkun Fang Haosheng He Jiamin Liu Rejun Fang New insights into the mechanisms of iron absorption: Iron dextran uptake in the intestines of weaned pigs through glucose transporter 5 (GLUT5) and divalent metal transporter 1 (DMT1) transporters Animal Nutrition Iron dextran Weaned piglet Divalent metal transporter 1 Glucose transporter 5 Intestinal iron absorption |
| title | New insights into the mechanisms of iron absorption: Iron dextran uptake in the intestines of weaned pigs through glucose transporter 5 (GLUT5) and divalent metal transporter 1 (DMT1) transporters |
| title_full | New insights into the mechanisms of iron absorption: Iron dextran uptake in the intestines of weaned pigs through glucose transporter 5 (GLUT5) and divalent metal transporter 1 (DMT1) transporters |
| title_fullStr | New insights into the mechanisms of iron absorption: Iron dextran uptake in the intestines of weaned pigs through glucose transporter 5 (GLUT5) and divalent metal transporter 1 (DMT1) transporters |
| title_full_unstemmed | New insights into the mechanisms of iron absorption: Iron dextran uptake in the intestines of weaned pigs through glucose transporter 5 (GLUT5) and divalent metal transporter 1 (DMT1) transporters |
| title_short | New insights into the mechanisms of iron absorption: Iron dextran uptake in the intestines of weaned pigs through glucose transporter 5 (GLUT5) and divalent metal transporter 1 (DMT1) transporters |
| title_sort | new insights into the mechanisms of iron absorption iron dextran uptake in the intestines of weaned pigs through glucose transporter 5 glut5 and divalent metal transporter 1 dmt1 transporters |
| topic | Iron dextran Weaned piglet Divalent metal transporter 1 Glucose transporter 5 Intestinal iron absorption |
| url | http://www.sciencedirect.com/science/article/pii/S2405654524001008 |
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