Generation of an induced pluripotent stem cell line (NCKDi006-A) from a 34-year-old patient with focal segmental glomerulosclerosis carrying a heterozygous mutation in the TRPC6 gene

Focal segmental glomerulosclerosis (FSGS) is a common pathological nephrotic syndrome. The ion channel protein TRPC6 is associated mainly with the late-onset form of autosomal dominant familial FSGS. We identified a heterozygous mutation in the TRPC6 gene in an FSGS patient with a family history of...

Full description

Saved in:
Bibliographic Details
Main Authors: Yiqing Wang, Minghao Yu, Zhihong Liu, Gang Wang
Format: Article
Language:English
Published: Elsevier 2025-04-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506125000376
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850041698252488704
author Yiqing Wang
Minghao Yu
Zhihong Liu
Gang Wang
author_facet Yiqing Wang
Minghao Yu
Zhihong Liu
Gang Wang
author_sort Yiqing Wang
collection DOAJ
description Focal segmental glomerulosclerosis (FSGS) is a common pathological nephrotic syndrome. The ion channel protein TRPC6 is associated mainly with the late-onset form of autosomal dominant familial FSGS. We identified a heterozygous mutation in the TRPC6 gene in an FSGS patient with a family history of end-stage renal disease (ESRD). Peripheral blood mononuclear cells (PBMCs) were obtained from the patient, and induced pluripotent stem cell (iPSC) lines were successfully generated. The establishment of these iPSC lines will provide a basis for further research into the pathogenesis of this condition.
format Article
id doaj-art-d3cd9f3bf95c4b65b134d4d7eb99373a
institution DOAJ
issn 1873-5061
language English
publishDate 2025-04-01
publisher Elsevier
record_format Article
series Stem Cell Research
spelling doaj-art-d3cd9f3bf95c4b65b134d4d7eb99373a2025-08-20T02:55:42ZengElsevierStem Cell Research1873-50612025-04-018410368710.1016/j.scr.2025.103687Generation of an induced pluripotent stem cell line (NCKDi006-A) from a 34-year-old patient with focal segmental glomerulosclerosis carrying a heterozygous mutation in the TRPC6 geneYiqing Wang0Minghao Yu1Zhihong Liu2Gang Wang3Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China; National Clinical Research Center of Kidney Diseases, Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu 210002, China; Liangzhu Laboratory, Zhejiang University Medical Center, Hangzhou, Zhejiang 310058, ChinaNational Clinical Research Center of Kidney Diseases, Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu 210002, ChinaZhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China; National Clinical Research Center of Kidney Diseases, Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu 210002, China; Liangzhu Laboratory, Zhejiang University Medical Center, Hangzhou, Zhejiang 310058, China; Corresponding authors at: National Clinical Research Center of Kidney Diseases, Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu, 210002, China.National Clinical Research Center of Kidney Diseases, Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu 210002, China; Corresponding authors at: National Clinical Research Center of Kidney Diseases, Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu, 210002, China.Focal segmental glomerulosclerosis (FSGS) is a common pathological nephrotic syndrome. The ion channel protein TRPC6 is associated mainly with the late-onset form of autosomal dominant familial FSGS. We identified a heterozygous mutation in the TRPC6 gene in an FSGS patient with a family history of end-stage renal disease (ESRD). Peripheral blood mononuclear cells (PBMCs) were obtained from the patient, and induced pluripotent stem cell (iPSC) lines were successfully generated. The establishment of these iPSC lines will provide a basis for further research into the pathogenesis of this condition.http://www.sciencedirect.com/science/article/pii/S1873506125000376
spellingShingle Yiqing Wang
Minghao Yu
Zhihong Liu
Gang Wang
Generation of an induced pluripotent stem cell line (NCKDi006-A) from a 34-year-old patient with focal segmental glomerulosclerosis carrying a heterozygous mutation in the TRPC6 gene
Stem Cell Research
title Generation of an induced pluripotent stem cell line (NCKDi006-A) from a 34-year-old patient with focal segmental glomerulosclerosis carrying a heterozygous mutation in the TRPC6 gene
title_full Generation of an induced pluripotent stem cell line (NCKDi006-A) from a 34-year-old patient with focal segmental glomerulosclerosis carrying a heterozygous mutation in the TRPC6 gene
title_fullStr Generation of an induced pluripotent stem cell line (NCKDi006-A) from a 34-year-old patient with focal segmental glomerulosclerosis carrying a heterozygous mutation in the TRPC6 gene
title_full_unstemmed Generation of an induced pluripotent stem cell line (NCKDi006-A) from a 34-year-old patient with focal segmental glomerulosclerosis carrying a heterozygous mutation in the TRPC6 gene
title_short Generation of an induced pluripotent stem cell line (NCKDi006-A) from a 34-year-old patient with focal segmental glomerulosclerosis carrying a heterozygous mutation in the TRPC6 gene
title_sort generation of an induced pluripotent stem cell line nckdi006 a from a 34 year old patient with focal segmental glomerulosclerosis carrying a heterozygous mutation in the trpc6 gene
url http://www.sciencedirect.com/science/article/pii/S1873506125000376
work_keys_str_mv AT yiqingwang generationofaninducedpluripotentstemcelllinenckdi006afroma34yearoldpatientwithfocalsegmentalglomerulosclerosiscarryingaheterozygousmutationinthetrpc6gene
AT minghaoyu generationofaninducedpluripotentstemcelllinenckdi006afroma34yearoldpatientwithfocalsegmentalglomerulosclerosiscarryingaheterozygousmutationinthetrpc6gene
AT zhihongliu generationofaninducedpluripotentstemcelllinenckdi006afroma34yearoldpatientwithfocalsegmentalglomerulosclerosiscarryingaheterozygousmutationinthetrpc6gene
AT gangwang generationofaninducedpluripotentstemcelllinenckdi006afroma34yearoldpatientwithfocalsegmentalglomerulosclerosiscarryingaheterozygousmutationinthetrpc6gene