Assessing Genetic Variability and Population Structure of <i>Alnus glutinosa</i> (Black Alder) in Kazakhstan Using SSR Markers

<i>Alnus glutinosa</i> plays a crucial role in flood control, riverbank stabilization, and water purification. Recognized for its ecological significance, it is listed in the Red Book of Kazakhstan. This study investigated the genetic variability of <i>A. glutinosa</i> popula...

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Main Authors: Aidana Nurtaza, Damira Dyussembekova, Alexandr Shevtsov, Symbat Islamova, Indira Samatova, Saule Koblanova, Olga Borodulina, Almagul Kakimzhanova
Format: Article
Language:English
Published: MDPI AG 2024-10-01
Series:Plants
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Online Access:https://www.mdpi.com/2223-7747/13/21/3032
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author Aidana Nurtaza
Damira Dyussembekova
Alexandr Shevtsov
Symbat Islamova
Indira Samatova
Saule Koblanova
Olga Borodulina
Almagul Kakimzhanova
author_facet Aidana Nurtaza
Damira Dyussembekova
Alexandr Shevtsov
Symbat Islamova
Indira Samatova
Saule Koblanova
Olga Borodulina
Almagul Kakimzhanova
author_sort Aidana Nurtaza
collection DOAJ
description <i>Alnus glutinosa</i> plays a crucial role in flood control, riverbank stabilization, and water purification. Recognized for its ecological significance, it is listed in the Red Book of Kazakhstan. This study investigated the genetic variability of <i>A. glutinosa</i> populations in Kazakhstan, analyzing 78 trees from seven populations in the Bayanaul mountain forest massif and the northern Turgay regions using 12 SSR markers. The study identified an average of 6.3 alleles and 2.783 effective alleles, as well as observed and expected heterozygosities of 0.570 and 0.562, respectively, reflecting genetic diversity. Among the populations, KS1 (northern Turgay) and PVL3 (Bayanaul) displayed the highest diversity, while PVL5 (Bayanaul) showed slightly lower diversity. The analysis of molecular variance results indicated that 86% of the genetic diversity occurred within populations, with 14% attributed to differences between populations. A UPGMA tree based on Nei’s genetic distance revealed three distinct clusters, suggesting geographically structured genetic variability in <i>A. glutinosa</i> populations.
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spelling doaj-art-d3cbd207763f49ecb26db4ea78f27e242025-08-20T02:13:14ZengMDPI AGPlants2223-77472024-10-011321303210.3390/plants13213032Assessing Genetic Variability and Population Structure of <i>Alnus glutinosa</i> (Black Alder) in Kazakhstan Using SSR MarkersAidana Nurtaza0Damira Dyussembekova1Alexandr Shevtsov2Symbat Islamova3Indira Samatova4Saule Koblanova5Olga Borodulina6Almagul Kakimzhanova7National Center for Biotechnology, 13/5, Korgalzhyn Road, Astana 010000, KazakhstanNational Center for Biotechnology, 13/5, Korgalzhyn Road, Astana 010000, KazakhstanNational Center for Biotechnology, 13/5, Korgalzhyn Road, Astana 010000, KazakhstanNational Center for Biotechnology, 13/5, Korgalzhyn Road, Astana 010000, KazakhstanNational Center for Biotechnology, 13/5, Korgalzhyn Road, Astana 010000, KazakhstanNational Center for Biotechnology, 13/5, Korgalzhyn Road, Astana 010000, KazakhstanDepartment of Natural Sciences, Kostanay Regional University, 47 Baytursynov Str., Kostanay 110000, KazakhstanNational Center for Biotechnology, 13/5, Korgalzhyn Road, Astana 010000, Kazakhstan<i>Alnus glutinosa</i> plays a crucial role in flood control, riverbank stabilization, and water purification. Recognized for its ecological significance, it is listed in the Red Book of Kazakhstan. This study investigated the genetic variability of <i>A. glutinosa</i> populations in Kazakhstan, analyzing 78 trees from seven populations in the Bayanaul mountain forest massif and the northern Turgay regions using 12 SSR markers. The study identified an average of 6.3 alleles and 2.783 effective alleles, as well as observed and expected heterozygosities of 0.570 and 0.562, respectively, reflecting genetic diversity. Among the populations, KS1 (northern Turgay) and PVL3 (Bayanaul) displayed the highest diversity, while PVL5 (Bayanaul) showed slightly lower diversity. The analysis of molecular variance results indicated that 86% of the genetic diversity occurred within populations, with 14% attributed to differences between populations. A UPGMA tree based on Nei’s genetic distance revealed three distinct clusters, suggesting geographically structured genetic variability in <i>A. glutinosa</i> populations.https://www.mdpi.com/2223-7747/13/21/3032<i>Alnus glutinosa</i>SSRgenetic diversitypopulation structure
spellingShingle Aidana Nurtaza
Damira Dyussembekova
Alexandr Shevtsov
Symbat Islamova
Indira Samatova
Saule Koblanova
Olga Borodulina
Almagul Kakimzhanova
Assessing Genetic Variability and Population Structure of <i>Alnus glutinosa</i> (Black Alder) in Kazakhstan Using SSR Markers
Plants
<i>Alnus glutinosa</i>
SSR
genetic diversity
population structure
title Assessing Genetic Variability and Population Structure of <i>Alnus glutinosa</i> (Black Alder) in Kazakhstan Using SSR Markers
title_full Assessing Genetic Variability and Population Structure of <i>Alnus glutinosa</i> (Black Alder) in Kazakhstan Using SSR Markers
title_fullStr Assessing Genetic Variability and Population Structure of <i>Alnus glutinosa</i> (Black Alder) in Kazakhstan Using SSR Markers
title_full_unstemmed Assessing Genetic Variability and Population Structure of <i>Alnus glutinosa</i> (Black Alder) in Kazakhstan Using SSR Markers
title_short Assessing Genetic Variability and Population Structure of <i>Alnus glutinosa</i> (Black Alder) in Kazakhstan Using SSR Markers
title_sort assessing genetic variability and population structure of i alnus glutinosa i black alder in kazakhstan using ssr markers
topic <i>Alnus glutinosa</i>
SSR
genetic diversity
population structure
url https://www.mdpi.com/2223-7747/13/21/3032
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