Hemolysis rates of three red blood cell components at the end of storage: a 5-year retrospective study

[Objective] To evaluate the suitability of the existing hemolysis rate standards for locally processed red blood cell components by retrospectively analyzing 5-year hemolysis rate data at the end of storage. [Methods] A total of 720 blood samples of three types of red blood cell components from our...

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Main Authors: LU Zhenping, LIU Fufa, KANG Meiyan, WU Xianbin, WANG Yanting, LONG Xing, QIU Xinlu, LI Jin
Format: Article
Language:zho
Published: Institute of Blood Transfusion of Chinese Academy of Medical Sciences 2025-06-01
Series:Zhongguo shuxue zazhi
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Online Access:https://www.cjbt.cn/thesisDetails#10.13303/j.cjbt.issn.1004-549x.2025.06.013&lang=en
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author LU Zhenping
LIU Fufa
KANG Meiyan
WU Xianbin
WANG Yanting
LONG Xing
QIU Xinlu
LI Jin
author_facet LU Zhenping
LIU Fufa
KANG Meiyan
WU Xianbin
WANG Yanting
LONG Xing
QIU Xinlu
LI Jin
author_sort LU Zhenping
collection DOAJ
description [Objective] To evaluate the suitability of the existing hemolysis rate standards for locally processed red blood cell components by retrospectively analyzing 5-year hemolysis rate data at the end of storage. [Methods] A total of 720 blood samples of three types of red blood cell components from our blood station from January 2019 to December 2023 were collected. Parameters included hemoglobin concentration (Hb), hematocrit (Hct), and free hemoglobin concentration (fHb). Hemolysis rate were taken as the control standard of 0.8% in accordance with the national standard. The hemolysis rates were compared against the national standard threshold of 0.8% (GB18469-2012), and annual trends of the detection parameters were observed. [Results] The hemolysis rates (x-+s,%) of leukocyte-depleted whole blood at the end of storage were (0.038±0.023 8) in 2019, (0.049±0.039 5) in 2020, (0.043±0.040 7) in 2021, (0.049±0.030 7) in 2022, and (0.058±0.054 8) in 2023, respectively; The hemolysis rates (x-+s" />,%) of leukocyte-depleted suspended red blood cells at the end of storage were (0.093±0.050 2) in 2019, (0.086±0.049 5) in 2020, (0.123±0.072 3) in 2021, (0.122±0.052 1) in 2022, and (0.106±0.058 6) in 2023, respectively; The hemolysis rates (x-+s,%) of washed red blood cells at the end of storage were (0.127±0.038 2) in 2019, (0.150±0.066 5) in 2020, (0.121±0.052 2) in 2021, (0.124±0.038 9) in 2022, and (0.128±0.044 3) in 2023, respectively. [Conclusion] Hemolysis rates at the end of blood storage of three red blood cell components were significantly lower than the limits specified in Quality Requirements for Whole Blood and Components (GB18469-2012), as well as standards from the EU, AABB and the United States. The results demonstrate excellent product quality control. A regional internal control standard of <0.2% is proposed for hemolysis rates at the end of storage.
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publisher Institute of Blood Transfusion of Chinese Academy of Medical Sciences
record_format Article
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spelling doaj-art-d3c53406ec944f9dad68ac6ca2cefcef2025-08-20T02:44:28ZzhoInstitute of Blood Transfusion of Chinese Academy of Medical SciencesZhongguo shuxue zazhi1004-549X2025-06-0138682883210.13303/j.cjbt.issn.1004-549x.2025.06.0131004-549X(2025)6-0828-05Hemolysis rates of three red blood cell components at the end of storage: a 5-year retrospective studyLU Zhenping0LIU Fufa1KANG Meiyan2WU Xianbin3WANG Yanting4LONG Xing5QIU Xinlu6LI Jin7Ganzhou Central Blood Station, Ganzhou 341000, ChinaGanzhou Central Blood Station, Ganzhou 341000, ChinaGanzhou Central Blood Station, Ganzhou 341000, ChinaGanzhou Central Blood Station, Ganzhou 341000, ChinaGanzhou Central Blood Station, Ganzhou 341000, ChinaGanzhou Central Blood Station, Ganzhou 341000, ChinaGanzhou Central Blood Station, Ganzhou 341000, ChinaGanzhou Central Blood Station, Ganzhou 341000, China[Objective] To evaluate the suitability of the existing hemolysis rate standards for locally processed red blood cell components by retrospectively analyzing 5-year hemolysis rate data at the end of storage. [Methods] A total of 720 blood samples of three types of red blood cell components from our blood station from January 2019 to December 2023 were collected. Parameters included hemoglobin concentration (Hb), hematocrit (Hct), and free hemoglobin concentration (fHb). Hemolysis rate were taken as the control standard of 0.8% in accordance with the national standard. The hemolysis rates were compared against the national standard threshold of 0.8% (GB18469-2012), and annual trends of the detection parameters were observed. [Results] The hemolysis rates (x-+s,%) of leukocyte-depleted whole blood at the end of storage were (0.038±0.023 8) in 2019, (0.049±0.039 5) in 2020, (0.043±0.040 7) in 2021, (0.049±0.030 7) in 2022, and (0.058±0.054 8) in 2023, respectively; The hemolysis rates (x-+s" />,%) of leukocyte-depleted suspended red blood cells at the end of storage were (0.093±0.050 2) in 2019, (0.086±0.049 5) in 2020, (0.123±0.072 3) in 2021, (0.122±0.052 1) in 2022, and (0.106±0.058 6) in 2023, respectively; The hemolysis rates (x-+s,%) of washed red blood cells at the end of storage were (0.127±0.038 2) in 2019, (0.150±0.066 5) in 2020, (0.121±0.052 2) in 2021, (0.124±0.038 9) in 2022, and (0.128±0.044 3) in 2023, respectively. [Conclusion] Hemolysis rates at the end of blood storage of three red blood cell components were significantly lower than the limits specified in Quality Requirements for Whole Blood and Components (GB18469-2012), as well as standards from the EU, AABB and the United States. The results demonstrate excellent product quality control. A regional internal control standard of <0.2% is proposed for hemolysis rates at the end of storage.https://www.cjbt.cn/thesisDetails#10.13303/j.cjbt.issn.1004-549x.2025.06.013&lang=enred blood cell componentshemolysis rate at the end of storagequality standard
spellingShingle LU Zhenping
LIU Fufa
KANG Meiyan
WU Xianbin
WANG Yanting
LONG Xing
QIU Xinlu
LI Jin
Hemolysis rates of three red blood cell components at the end of storage: a 5-year retrospective study
Zhongguo shuxue zazhi
red blood cell components
hemolysis rate at the end of storage
quality standard
title Hemolysis rates of three red blood cell components at the end of storage: a 5-year retrospective study
title_full Hemolysis rates of three red blood cell components at the end of storage: a 5-year retrospective study
title_fullStr Hemolysis rates of three red blood cell components at the end of storage: a 5-year retrospective study
title_full_unstemmed Hemolysis rates of three red blood cell components at the end of storage: a 5-year retrospective study
title_short Hemolysis rates of three red blood cell components at the end of storage: a 5-year retrospective study
title_sort hemolysis rates of three red blood cell components at the end of storage a 5 year retrospective study
topic red blood cell components
hemolysis rate at the end of storage
quality standard
url https://www.cjbt.cn/thesisDetails#10.13303/j.cjbt.issn.1004-549x.2025.06.013&lang=en
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AT liufufa hemolysisratesofthreeredbloodcellcomponentsattheendofstoragea5yearretrospectivestudy
AT kangmeiyan hemolysisratesofthreeredbloodcellcomponentsattheendofstoragea5yearretrospectivestudy
AT wuxianbin hemolysisratesofthreeredbloodcellcomponentsattheendofstoragea5yearretrospectivestudy
AT wangyanting hemolysisratesofthreeredbloodcellcomponentsattheendofstoragea5yearretrospectivestudy
AT longxing hemolysisratesofthreeredbloodcellcomponentsattheendofstoragea5yearretrospectivestudy
AT qiuxinlu hemolysisratesofthreeredbloodcellcomponentsattheendofstoragea5yearretrospectivestudy
AT lijin hemolysisratesofthreeredbloodcellcomponentsattheendofstoragea5yearretrospectivestudy