Allicin-loaded hydrogel enhances the viability of multiterritory perforator flap
The multiterritory perforator flap has gained increasing clinical adoption in limb reconstruction and wound repair due to its procedural simplicity and distinct clinical advantages. Allicin, a multifunctional phytochemical renowned for its potent antioxidant properties, faces significant limitations...
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Elsevier
2025-08-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006425005964 |
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| author | Xuankuai Chen Xingxing Zhou Kejian Fu Xibing Zhao Xiang Hu Sheng Cheng Renhao Jiang Chengji Dong Long Wang Hongqiang Wu Weiyang Gao Liyan Shen |
| author_facet | Xuankuai Chen Xingxing Zhou Kejian Fu Xibing Zhao Xiang Hu Sheng Cheng Renhao Jiang Chengji Dong Long Wang Hongqiang Wu Weiyang Gao Liyan Shen |
| author_sort | Xuankuai Chen |
| collection | DOAJ |
| description | The multiterritory perforator flap has gained increasing clinical adoption in limb reconstruction and wound repair due to its procedural simplicity and distinct clinical advantages. Allicin, a multifunctional phytochemical renowned for its potent antioxidant properties, faces significant limitations due to rapid metabolic clearance, which substantially diminishes its therapeutic efficacy. Recent advances in hydrogel technology have highlighted their potential as advanced wound dressings, particularly through their capacity for sustained drug release. Our research synthesized HAMA-400K-Allicin (a hyaluronic acid methacryloyl hydrogel incorporating allicin), aiming to improve perforator flaps' survival rate by long-term oxidative stress alleviation. Characterization studies demonstrated that the synthesized hydrogel exhibits a densely crosslinked network architecture, robust tissue adhesion, and photosensitive properties. Notably, HAMA-400K-Allicin exhibited excellent biocompatibility and facilitated sustained allicin release for up to one week. Experimental validation revealed that both allicin and HAMA-400K-Allicin significantly improved perforator flap survival rates in rat models, with the hydrogel formulation demonstrating superior therapeutic outcomes. Mechanistic investigations further suggested that HAMA-400K-Allicin promotes flap survival through dual activation of the PI3K/AKT and HO-1/NRF2 signaling pathways. Ultimately, in a rat model, HAMA-400K-Allicin exhibited a robust effect in promoting perforator flap survival. In conclusion, this hydrogel system combines straightforward manufacturing processes with high clinical efficacy, positioning it as a promising solution for complex wound management scenarios. |
| format | Article |
| id | doaj-art-d3c4bbb36c724200ac2c5a56f29dd112 |
| institution | Kabale University |
| issn | 2590-0064 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj-art-d3c4bbb36c724200ac2c5a56f29dd1122025-08-20T03:27:05ZengElsevierMaterials Today Bio2590-00642025-08-013310202610.1016/j.mtbio.2025.102026Allicin-loaded hydrogel enhances the viability of multiterritory perforator flapXuankuai Chen0Xingxing Zhou1Kejian Fu2Xibing Zhao3Xiang Hu4Sheng Cheng5Renhao Jiang6Chengji Dong7Long Wang8Hongqiang Wu9Weiyang Gao10Liyan Shen11Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, 325027, China; Key Laboratory of Orthopaedics of Zhejiang Province, Wenzhou, 325027, China; The Second School of Medicine, Wenzhou Medical University, Wenzhou, 325027, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, 325027, China; Key Laboratory of Orthopaedics of Zhejiang Province, Wenzhou, 325027, China; The Second School of Medicine, Wenzhou Medical University, Wenzhou, 325027, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, 325027, China; Key Laboratory of Orthopaedics of Zhejiang Province, Wenzhou, 325027, China; The Second School of Medicine, Wenzhou Medical University, Wenzhou, 325027, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, 325027, China; Key Laboratory of Orthopaedics of Zhejiang Province, Wenzhou, 325027, China; The Second School of Medicine, Wenzhou Medical University, Wenzhou, 325027, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, 325027, China; Key Laboratory of Orthopaedics of Zhejiang Province, Wenzhou, 325027, China; The Second School of Medicine, Wenzhou Medical University, Wenzhou, 325027, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, 325027, China; Key Laboratory of Orthopaedics of Zhejiang Province, Wenzhou, 325027, China; The Second School of Medicine, Wenzhou Medical University, Wenzhou, 325027, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, 325027, China; Key Laboratory of Orthopaedics of Zhejiang Province, Wenzhou, 325027, China; The Second School of Medicine, Wenzhou Medical University, Wenzhou, 325027, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, 325027, China; Key Laboratory of Orthopaedics of Zhejiang Province, Wenzhou, 325027, China; The Second School of Medicine, Wenzhou Medical University, Wenzhou, 325027, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, 325027, China; Key Laboratory of Orthopaedics of Zhejiang Province, Wenzhou, 325027, China; The Second School of Medicine, Wenzhou Medical University, Wenzhou, 325027, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, 325027, China; Key Laboratory of Orthopaedics of Zhejiang Province, Wenzhou, 325027, China; The Second School of Medicine, Wenzhou Medical University, Wenzhou, 325027, China; Corresponding author. Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, 325027, China.Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, 325027, China; Key Laboratory of Orthopaedics of Zhejiang Province, Wenzhou, 325027, China; The Second School of Medicine, Wenzhou Medical University, Wenzhou, 325027, China; Corresponding author. Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, 325027, China.Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, 325027, China; Key Laboratory of Orthopaedics of Zhejiang Province, Wenzhou, 325027, China; The Second School of Medicine, Wenzhou Medical University, Wenzhou, 325027, China; Corresponding author. Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, 325027, China.The multiterritory perforator flap has gained increasing clinical adoption in limb reconstruction and wound repair due to its procedural simplicity and distinct clinical advantages. Allicin, a multifunctional phytochemical renowned for its potent antioxidant properties, faces significant limitations due to rapid metabolic clearance, which substantially diminishes its therapeutic efficacy. Recent advances in hydrogel technology have highlighted their potential as advanced wound dressings, particularly through their capacity for sustained drug release. Our research synthesized HAMA-400K-Allicin (a hyaluronic acid methacryloyl hydrogel incorporating allicin), aiming to improve perforator flaps' survival rate by long-term oxidative stress alleviation. Characterization studies demonstrated that the synthesized hydrogel exhibits a densely crosslinked network architecture, robust tissue adhesion, and photosensitive properties. Notably, HAMA-400K-Allicin exhibited excellent biocompatibility and facilitated sustained allicin release for up to one week. Experimental validation revealed that both allicin and HAMA-400K-Allicin significantly improved perforator flap survival rates in rat models, with the hydrogel formulation demonstrating superior therapeutic outcomes. Mechanistic investigations further suggested that HAMA-400K-Allicin promotes flap survival through dual activation of the PI3K/AKT and HO-1/NRF2 signaling pathways. Ultimately, in a rat model, HAMA-400K-Allicin exhibited a robust effect in promoting perforator flap survival. In conclusion, this hydrogel system combines straightforward manufacturing processes with high clinical efficacy, positioning it as a promising solution for complex wound management scenarios.http://www.sciencedirect.com/science/article/pii/S2590006425005964Multiterritory perforator flapHydrogelOxidative stressPI3K/AKTNRF2/HO-1cGRP |
| spellingShingle | Xuankuai Chen Xingxing Zhou Kejian Fu Xibing Zhao Xiang Hu Sheng Cheng Renhao Jiang Chengji Dong Long Wang Hongqiang Wu Weiyang Gao Liyan Shen Allicin-loaded hydrogel enhances the viability of multiterritory perforator flap Materials Today Bio Multiterritory perforator flap Hydrogel Oxidative stress PI3K/AKT NRF2/HO-1 cGRP |
| title | Allicin-loaded hydrogel enhances the viability of multiterritory perforator flap |
| title_full | Allicin-loaded hydrogel enhances the viability of multiterritory perforator flap |
| title_fullStr | Allicin-loaded hydrogel enhances the viability of multiterritory perforator flap |
| title_full_unstemmed | Allicin-loaded hydrogel enhances the viability of multiterritory perforator flap |
| title_short | Allicin-loaded hydrogel enhances the viability of multiterritory perforator flap |
| title_sort | allicin loaded hydrogel enhances the viability of multiterritory perforator flap |
| topic | Multiterritory perforator flap Hydrogel Oxidative stress PI3K/AKT NRF2/HO-1 cGRP |
| url | http://www.sciencedirect.com/science/article/pii/S2590006425005964 |
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