Effect of ozone gas on cultures of Candida albicans and Aspergillus fumigatus: evaluation of two ozonation devices
Introduction: Candida albicans and Aspergillus fumigatus are two important agents of Healthcare-associated infections. This study aimed to evaluate the antifungal activity of ozone (O3) gas produced by two commercial devices against cultures of these two species. Methodology: Sterile plastic plat...
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The Journal of Infection in Developing Countries
2024-03-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/15344 |
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| author | Maicon Henrique Caetano Adriano Menis Ferreira Denise de Andrade Álvaro Francisco Lopes de Sousa Liliane Moretti Carneiro Alessandra Lyrio Barbosa Herica Emilia Félix de Carvalho Marcelo Alessandro Rigotti Margarete Teresa Gottardo de Almeida |
| author_facet | Maicon Henrique Caetano Adriano Menis Ferreira Denise de Andrade Álvaro Francisco Lopes de Sousa Liliane Moretti Carneiro Alessandra Lyrio Barbosa Herica Emilia Félix de Carvalho Marcelo Alessandro Rigotti Margarete Teresa Gottardo de Almeida |
| author_sort | Maicon Henrique Caetano |
| collection | DOAJ |
| description |
Introduction: Candida albicans and Aspergillus fumigatus are two important agents of Healthcare-associated infections. This study aimed to evaluate the antifungal activity of ozone (O3) gas produced by two commercial devices against cultures of these two species.
Methodology: Sterile plastic plates were inoculated with C. albicans and A. fumigatus and placed on a countertop at three distances (30 cm, 1 m, and 2 m) and three positions in relation to the wall (near, middle, and away), considering the source of O3. Plates were exposed to O3 for one hour and incubated. After incubation, the counting of colony-forming units was performed. As a control, an inoculated plate was incubated, without being exposed to O3. Tests were carried out with two different devices (namely, Mod.I and Mod.II), with the air conditioner on and off, in triplicate.
Results: Both devices showed antifungal activity. Mod. I presented better results, due to a higher flow rate. The best activity was on plates at 30 cm, middle position. Contrarily, on plates at 2m, near the wall, the inhibition activity was lower. The best results were obtained with the air conditioner off. Candida albicans was more sensitive to O3 than A. fumigatus.
Conclusions: This method of decontamination by O3 gas shows potential due to its fast and easy execution. The establishment of new protocols for hygiene and hospital disinfection using this approach should be considered, which may reduce environmental contamination by fungi and, consequently, the burden of fungal infections.
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| format | Article |
| id | doaj-art-d3b97b5348ee43fabae523e526f5ceef |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2024-03-01 |
| publisher | The Journal of Infection in Developing Countries |
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| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-d3b97b5348ee43fabae523e526f5ceef2025-08-20T02:27:22ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802024-03-01180310.3855/jidc.15344Effect of ozone gas on cultures of Candida albicans and Aspergillus fumigatus: evaluation of two ozonation devicesMaicon Henrique Caetano0Adriano Menis Ferreira1Denise de Andrade2Álvaro Francisco Lopes de Sousa3Liliane Moretti Carneiro4Alessandra Lyrio Barbosa5Herica Emilia Félix de Carvalho6Marcelo Alessandro Rigotti7Margarete Teresa Gottardo de Almeida8Universidade Estadual Paulista (UNESP), Instituto de Biociências, Letras e Ciências Exatas (Ibilce), Campus São José do Rio Preto, São Paulo, BrazilUniversidade Federal de Mato Grosso do Sul, Três Lagoas, Mato Grosso do Sul, BrazilHuman Exposome and Infectious Diseases Network (HEID); Universidade de São Paulo, Escola de Enfermagem de Ribeirão Preto, Ribeirão Preto, São Paulo, BrazilHuman Exposome and Infectious Diseases Network (HEID); Universidade de São Paulo, Escola de Enfermagem de Ribeirão Preto, Ribeirão Preto, São Paulo, BrazilUniversidade Federal de Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, BrazilUniversidade Federal de Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, BrazilHuman Exposome and Infectious Diseases Network (HEID); Universidade de São Paulo, Escola de Enfermagem de Ribeirão Preto, Ribeirão Preto, São Paulo, BrazilUniversidade Federal de Mato Grosso do Sul, Três Lagoas, Mato Grosso do Sul, BrazilFaculdade de Medicina de São José do Rio Preto, São José do Rio Preto, São Paulo, Brazil Introduction: Candida albicans and Aspergillus fumigatus are two important agents of Healthcare-associated infections. This study aimed to evaluate the antifungal activity of ozone (O3) gas produced by two commercial devices against cultures of these two species. Methodology: Sterile plastic plates were inoculated with C. albicans and A. fumigatus and placed on a countertop at three distances (30 cm, 1 m, and 2 m) and three positions in relation to the wall (near, middle, and away), considering the source of O3. Plates were exposed to O3 for one hour and incubated. After incubation, the counting of colony-forming units was performed. As a control, an inoculated plate was incubated, without being exposed to O3. Tests were carried out with two different devices (namely, Mod.I and Mod.II), with the air conditioner on and off, in triplicate. Results: Both devices showed antifungal activity. Mod. I presented better results, due to a higher flow rate. The best activity was on plates at 30 cm, middle position. Contrarily, on plates at 2m, near the wall, the inhibition activity was lower. The best results were obtained with the air conditioner off. Candida albicans was more sensitive to O3 than A. fumigatus. Conclusions: This method of decontamination by O3 gas shows potential due to its fast and easy execution. The establishment of new protocols for hygiene and hospital disinfection using this approach should be considered, which may reduce environmental contamination by fungi and, consequently, the burden of fungal infections. https://jidc.org/index.php/journal/article/view/15344OzoneozonationCandida albicansAspergillus fumigatusdisinfection |
| spellingShingle | Maicon Henrique Caetano Adriano Menis Ferreira Denise de Andrade Álvaro Francisco Lopes de Sousa Liliane Moretti Carneiro Alessandra Lyrio Barbosa Herica Emilia Félix de Carvalho Marcelo Alessandro Rigotti Margarete Teresa Gottardo de Almeida Effect of ozone gas on cultures of Candida albicans and Aspergillus fumigatus: evaluation of two ozonation devices Journal of Infection in Developing Countries Ozone ozonation Candida albicans Aspergillus fumigatus disinfection |
| title | Effect of ozone gas on cultures of Candida albicans and Aspergillus fumigatus: evaluation of two ozonation devices |
| title_full | Effect of ozone gas on cultures of Candida albicans and Aspergillus fumigatus: evaluation of two ozonation devices |
| title_fullStr | Effect of ozone gas on cultures of Candida albicans and Aspergillus fumigatus: evaluation of two ozonation devices |
| title_full_unstemmed | Effect of ozone gas on cultures of Candida albicans and Aspergillus fumigatus: evaluation of two ozonation devices |
| title_short | Effect of ozone gas on cultures of Candida albicans and Aspergillus fumigatus: evaluation of two ozonation devices |
| title_sort | effect of ozone gas on cultures of candida albicans and aspergillus fumigatus evaluation of two ozonation devices |
| topic | Ozone ozonation Candida albicans Aspergillus fumigatus disinfection |
| url | https://jidc.org/index.php/journal/article/view/15344 |
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