SERS-Driven Ceftriaxone Detection in Blood Plasma: A Protein Precipitation Approach
Accurate detection of antibiotics in biological samples is essential for clinical diagnoses and therapeutic drug monitoring. This research examines how proteins and other substances in blood plasma affect the detection of the antibiotic ceftriaxone using surface-enhanced Raman spectroscopy (SERS). W...
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MDPI AG
2024-10-01
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| Series: | Chemosensors |
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| Online Access: | https://www.mdpi.com/2227-9040/12/10/213 |
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| author | Aradhana Dwivedi Oleg Ryabchykov Chen Liu Edoardo Farnesi Michael Stenbæk Schmidt Thomas Bocklitz Jürgen Popp Dana Cialla-May |
| author_facet | Aradhana Dwivedi Oleg Ryabchykov Chen Liu Edoardo Farnesi Michael Stenbæk Schmidt Thomas Bocklitz Jürgen Popp Dana Cialla-May |
| author_sort | Aradhana Dwivedi |
| collection | DOAJ |
| description | Accurate detection of antibiotics in biological samples is essential for clinical diagnoses and therapeutic drug monitoring. This research examines how proteins and other substances in blood plasma affect the detection of the antibiotic ceftriaxone using surface-enhanced Raman spectroscopy (SERS). We detected ceftriaxone spiked in blood plasma without sample preparation within the range of 1 mg/mL to 50 µg/mL. By employing a pretreatment approach involving methanol-based protein precipitation to eliminate interfering substances from a spiked blood plasma solution, we could detect ceftriaxone down to 20 µg/mL. The comparative analysis demonstrates that the protein precipitation step enhances the sensitivity of SERS-based detection of drugs in the matrix blood plasma. The insights derived from this study are highly beneficial and can prove advantageous in developing new antibiotic detection methods that are both sensitive and selective in complex biological matrices. These methods can have important implications for clinical treatments. |
| format | Article |
| id | doaj-art-d3a20ad8e4ac4bf7ae3d216f66558405 |
| institution | OA Journals |
| issn | 2227-9040 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Chemosensors |
| spelling | doaj-art-d3a20ad8e4ac4bf7ae3d216f665584052025-08-20T02:10:59ZengMDPI AGChemosensors2227-90402024-10-01121021310.3390/chemosensors12100213SERS-Driven Ceftriaxone Detection in Blood Plasma: A Protein Precipitation ApproachAradhana Dwivedi0Oleg Ryabchykov1Chen Liu2Edoardo Farnesi3Michael Stenbæk Schmidt4Thomas Bocklitz5Jürgen Popp6Dana Cialla-May7Leibniz Institute of Photonic Technology, Member of Leibniz Health Technologies, Member of the Leibniz Centre for Photonics in Infection Research (LPI), Albert-Einstein-Straße 9, 07745 Jena, GermanyLeibniz Institute of Photonic Technology, Member of Leibniz Health Technologies, Member of the Leibniz Centre for Photonics in Infection Research (LPI), Albert-Einstein-Straße 9, 07745 Jena, GermanyLeibniz Institute of Photonic Technology, Member of Leibniz Health Technologies, Member of the Leibniz Centre for Photonics in Infection Research (LPI), Albert-Einstein-Straße 9, 07745 Jena, GermanyInstitute of Physical Chemistry (IPC) and Abbe Center of Photonics (ACP), Friedrich Schiller University Jena, Member of the Leibniz Centre for Photonics in Infection Research (LPI), Helmholtzweg 4, 07743 Jena, GermanySilmeco ApS, Kenny Drews Vej 101, 2450 Copenhagen, DenmarkLeibniz Institute of Photonic Technology, Member of Leibniz Health Technologies, Member of the Leibniz Centre for Photonics in Infection Research (LPI), Albert-Einstein-Straße 9, 07745 Jena, GermanyLeibniz Institute of Photonic Technology, Member of Leibniz Health Technologies, Member of the Leibniz Centre for Photonics in Infection Research (LPI), Albert-Einstein-Straße 9, 07745 Jena, GermanyLeibniz Institute of Photonic Technology, Member of Leibniz Health Technologies, Member of the Leibniz Centre for Photonics in Infection Research (LPI), Albert-Einstein-Straße 9, 07745 Jena, GermanyAccurate detection of antibiotics in biological samples is essential for clinical diagnoses and therapeutic drug monitoring. This research examines how proteins and other substances in blood plasma affect the detection of the antibiotic ceftriaxone using surface-enhanced Raman spectroscopy (SERS). We detected ceftriaxone spiked in blood plasma without sample preparation within the range of 1 mg/mL to 50 µg/mL. By employing a pretreatment approach involving methanol-based protein precipitation to eliminate interfering substances from a spiked blood plasma solution, we could detect ceftriaxone down to 20 µg/mL. The comparative analysis demonstrates that the protein precipitation step enhances the sensitivity of SERS-based detection of drugs in the matrix blood plasma. The insights derived from this study are highly beneficial and can prove advantageous in developing new antibiotic detection methods that are both sensitive and selective in complex biological matrices. These methods can have important implications for clinical treatments.https://www.mdpi.com/2227-9040/12/10/213therapeutic drug monitoring (TDM)ceftriaxoneblood plasma precipitation |
| spellingShingle | Aradhana Dwivedi Oleg Ryabchykov Chen Liu Edoardo Farnesi Michael Stenbæk Schmidt Thomas Bocklitz Jürgen Popp Dana Cialla-May SERS-Driven Ceftriaxone Detection in Blood Plasma: A Protein Precipitation Approach Chemosensors therapeutic drug monitoring (TDM) ceftriaxone blood plasma precipitation |
| title | SERS-Driven Ceftriaxone Detection in Blood Plasma: A Protein Precipitation Approach |
| title_full | SERS-Driven Ceftriaxone Detection in Blood Plasma: A Protein Precipitation Approach |
| title_fullStr | SERS-Driven Ceftriaxone Detection in Blood Plasma: A Protein Precipitation Approach |
| title_full_unstemmed | SERS-Driven Ceftriaxone Detection in Blood Plasma: A Protein Precipitation Approach |
| title_short | SERS-Driven Ceftriaxone Detection in Blood Plasma: A Protein Precipitation Approach |
| title_sort | sers driven ceftriaxone detection in blood plasma a protein precipitation approach |
| topic | therapeutic drug monitoring (TDM) ceftriaxone blood plasma precipitation |
| url | https://www.mdpi.com/2227-9040/12/10/213 |
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