Comparative Viability of <i>Francisella tularensis</i> subsp. <i>mediasiatica</i> and subsp. <i>holarctica</i> under <i>in vivo</i> and <i>in vitro</i> Conditions
The aim of the work was to compare the survival ability of virulent strains of tularemia microbe, mediasiatica and holarctica subspecies, endemic to Russia, in the aquatic environment and in the animal organism. Materials and methods. 11 virulent strains of various subspecies deposited in the State...
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| Format: | Article |
| Language: | Russian |
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Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”
2025-07-01
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| Series: | Проблемы особо опасных инфекций |
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| Online Access: | https://journal.microbe.ru/jour/article/view/2168 |
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| author | V. V. Evseeva G. M. Titareva I. V. Bakhteeva T. B. Kravchenko A. I. Borzilov T. I. Kombarova R. I. Mironova N. Yu. Krasavina G. Kh. Bazarova E. N. Rozhdestvensky V. S. Timofeev |
| author_facet | V. V. Evseeva G. M. Titareva I. V. Bakhteeva T. B. Kravchenko A. I. Borzilov T. I. Kombarova R. I. Mironova N. Yu. Krasavina G. Kh. Bazarova E. N. Rozhdestvensky V. S. Timofeev |
| author_sort | V. V. Evseeva |
| collection | DOAJ |
| description | The aim of the work was to compare the survival ability of virulent strains of tularemia microbe, mediasiatica and holarctica subspecies, endemic to Russia, in the aquatic environment and in the animal organism. Materials and methods. 11 virulent strains of various subspecies deposited in the State collection “SCPM-Obolensk” were used in the work. To assess the survival rate, bacterial cells of the studied strains at a concentration of 5·106 CFU/ml were incubated for 35 days in autoclaved tap water. The viability of Francisella tularensis bacterial cells was determined immediately before the experiment and then weekly by seeding on agar plates. To compare the holarctica and mediasiatica strains by in vivo survival ability during co-infection, the strain mixture was passaged three times through mice, the ratio of colonies of each strain in the spleen being calculated at each passage. Colony differentiation was performed by assessing the length of the Ft-M20 VNTR locus using PCR and subsequent gel electrophoresis. Results and discussion. Subsp. mediasiatica shows significantly lower ability to survive in water compared to subsp. holarctica. At the same time, analysis of the results of seeding after co-infection of mice with strains belonging to the two subspecies indicates that subsp. mediasiatica reproduces in the host organism more effectively than subsp. holarctica. |
| format | Article |
| id | doaj-art-d399d00b2fbf4428ac8e4a6010e95faa |
| institution | Kabale University |
| issn | 0370-1069 2658-719X |
| language | Russian |
| publishDate | 2025-07-01 |
| publisher | Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” |
| record_format | Article |
| series | Проблемы особо опасных инфекций |
| spelling | doaj-art-d399d00b2fbf4428ac8e4a6010e95faa2025-08-20T03:50:11ZrusFederal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”Проблемы особо опасных инфекций0370-10692658-719X2025-07-0102869410.21055/0370-1069-2025-2-86-941646Comparative Viability of <i>Francisella tularensis</i> subsp. <i>mediasiatica</i> and subsp. <i>holarctica</i> under <i>in vivo</i> and <i>in vitro</i> ConditionsV. V. Evseeva0G. M. Titareva1I. V. Bakhteeva2T. B. Kravchenko3A. I. Borzilov4T. I. Kombarova5R. I. Mironova6N. Yu. Krasavina7G. Kh. Bazarova8E. N. Rozhdestvensky9V. S. Timofeev10State Scientific Center of Applied Microbiology and BiotechnologyState Scientific Center of Applied Microbiology and BiotechnologyState Scientific Center of Applied Microbiology and BiotechnologyState Scientific Center of Applied Microbiology and BiotechnologyState Scientific Center of Applied Microbiology and BiotechnologyState Scientific Center of Applied Microbiology and BiotechnologyState Scientific Center of Applied Microbiology and BiotechnologyAltai Plague Control StationAltai Plague Control StationAltai Plague Control StationState Scientific Center of Applied Microbiology and BiotechnologyThe aim of the work was to compare the survival ability of virulent strains of tularemia microbe, mediasiatica and holarctica subspecies, endemic to Russia, in the aquatic environment and in the animal organism. Materials and methods. 11 virulent strains of various subspecies deposited in the State collection “SCPM-Obolensk” were used in the work. To assess the survival rate, bacterial cells of the studied strains at a concentration of 5·106 CFU/ml were incubated for 35 days in autoclaved tap water. The viability of Francisella tularensis bacterial cells was determined immediately before the experiment and then weekly by seeding on agar plates. To compare the holarctica and mediasiatica strains by in vivo survival ability during co-infection, the strain mixture was passaged three times through mice, the ratio of colonies of each strain in the spleen being calculated at each passage. Colony differentiation was performed by assessing the length of the Ft-M20 VNTR locus using PCR and subsequent gel electrophoresis. Results and discussion. Subsp. mediasiatica shows significantly lower ability to survive in water compared to subsp. holarctica. At the same time, analysis of the results of seeding after co-infection of mice with strains belonging to the two subspecies indicates that subsp. mediasiatica reproduces in the host organism more effectively than subsp. holarctica.https://journal.microbe.ru/jour/article/view/2168francisella tularensissubsp. mediasiaticaaquatic ecosystemsbacterial viability |
| spellingShingle | V. V. Evseeva G. M. Titareva I. V. Bakhteeva T. B. Kravchenko A. I. Borzilov T. I. Kombarova R. I. Mironova N. Yu. Krasavina G. Kh. Bazarova E. N. Rozhdestvensky V. S. Timofeev Comparative Viability of <i>Francisella tularensis</i> subsp. <i>mediasiatica</i> and subsp. <i>holarctica</i> under <i>in vivo</i> and <i>in vitro</i> Conditions Проблемы особо опасных инфекций francisella tularensis subsp. mediasiatica aquatic ecosystems bacterial viability |
| title | Comparative Viability of <i>Francisella tularensis</i> subsp. <i>mediasiatica</i> and subsp. <i>holarctica</i> under <i>in vivo</i> and <i>in vitro</i> Conditions |
| title_full | Comparative Viability of <i>Francisella tularensis</i> subsp. <i>mediasiatica</i> and subsp. <i>holarctica</i> under <i>in vivo</i> and <i>in vitro</i> Conditions |
| title_fullStr | Comparative Viability of <i>Francisella tularensis</i> subsp. <i>mediasiatica</i> and subsp. <i>holarctica</i> under <i>in vivo</i> and <i>in vitro</i> Conditions |
| title_full_unstemmed | Comparative Viability of <i>Francisella tularensis</i> subsp. <i>mediasiatica</i> and subsp. <i>holarctica</i> under <i>in vivo</i> and <i>in vitro</i> Conditions |
| title_short | Comparative Viability of <i>Francisella tularensis</i> subsp. <i>mediasiatica</i> and subsp. <i>holarctica</i> under <i>in vivo</i> and <i>in vitro</i> Conditions |
| title_sort | comparative viability of i francisella tularensis i subsp i mediasiatica i and subsp i holarctica i under i in vivo i and i in vitro i conditions |
| topic | francisella tularensis subsp. mediasiatica aquatic ecosystems bacterial viability |
| url | https://journal.microbe.ru/jour/article/view/2168 |
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