The Minimal Active Fragment of the Cry1Ai Toxin is Located Between 36I and 605I
The novel cry1Ai gene that cloned from Bacillus thuringiensis strain SC6H8 encoded a protein exhibiting strong toxicity against Plutella xylostella and Chilo suppressalis in our previous study. Using the available information for the active fragments of other Cry toxins, eight truncated fragments we...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2014-05-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311913605328 |
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| _version_ | 1849248737659977728 |
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| author | Zi-shan ZHOU Hui-yan LIN Ying LI Chang-long SHU Fu-ping SONG Jie ZHANG |
| author_facet | Zi-shan ZHOU Hui-yan LIN Ying LI Chang-long SHU Fu-ping SONG Jie ZHANG |
| author_sort | Zi-shan ZHOU |
| collection | DOAJ |
| description | The novel cry1Ai gene that cloned from Bacillus thuringiensis strain SC6H8 encoded a protein exhibiting strong toxicity against Plutella xylostella and Chilo suppressalis in our previous study. Using the available information for the active fragments of other Cry toxins, eight truncated fragments were constructed to identify the minimal active fragment of Cry1Ai. All truncated fragments were expressed in Escherichia coli strain BL21 (DE3), and the insecticidal activity against 2nd-instar P. xylostella larvae was assessed using full-length Cry1Ai as a positive control. The results indicate that the minimal active fragment of the Cry1Ai toxin against P. xylostella is located between amino acid residues 36I and 605I, which is smaller than the regions previously reported for Cry1A. The first two amino acids (34T and 35P) on helix α-1 and whole helix α-2 of domain I and sheet β-32 of domain III are necessary for Cry1Ai toxin to keep its toxicity against P. xylostella. |
| format | Article |
| id | doaj-art-d396f9c110de4e6e8246ffa0e3688c14 |
| institution | Kabale University |
| issn | 2095-3119 |
| language | English |
| publishDate | 2014-05-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-d396f9c110de4e6e8246ffa0e3688c142025-08-20T03:57:47ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192014-05-011351036104210.1016/S2095-3119(13)60532-8The Minimal Active Fragment of the Cry1Ai Toxin is Located Between 36I and 605IZi-shan ZHOU0Hui-yan LIN1Ying LI2Chang-long SHU3Fu-ping SONG4Jie ZHANG5State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaCorrespondence ZHANG Jie, Tel: +86-10-62815921, Fax: +86-10-62812642; State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaThe novel cry1Ai gene that cloned from Bacillus thuringiensis strain SC6H8 encoded a protein exhibiting strong toxicity against Plutella xylostella and Chilo suppressalis in our previous study. Using the available information for the active fragments of other Cry toxins, eight truncated fragments were constructed to identify the minimal active fragment of Cry1Ai. All truncated fragments were expressed in Escherichia coli strain BL21 (DE3), and the insecticidal activity against 2nd-instar P. xylostella larvae was assessed using full-length Cry1Ai as a positive control. The results indicate that the minimal active fragment of the Cry1Ai toxin against P. xylostella is located between amino acid residues 36I and 605I, which is smaller than the regions previously reported for Cry1A. The first two amino acids (34T and 35P) on helix α-1 and whole helix α-2 of domain I and sheet β-32 of domain III are necessary for Cry1Ai toxin to keep its toxicity against P. xylostella.http://www.sciencedirect.com/science/article/pii/S2095311913605328Bacillus thuringiensisCry1Ai toxinactive fragmenttoxicityPlutella xylostella |
| spellingShingle | Zi-shan ZHOU Hui-yan LIN Ying LI Chang-long SHU Fu-ping SONG Jie ZHANG The Minimal Active Fragment of the Cry1Ai Toxin is Located Between 36I and 605I Journal of Integrative Agriculture Bacillus thuringiensis Cry1Ai toxin active fragment toxicity Plutella xylostella |
| title | The Minimal Active Fragment of the Cry1Ai Toxin is Located Between 36I and 605I |
| title_full | The Minimal Active Fragment of the Cry1Ai Toxin is Located Between 36I and 605I |
| title_fullStr | The Minimal Active Fragment of the Cry1Ai Toxin is Located Between 36I and 605I |
| title_full_unstemmed | The Minimal Active Fragment of the Cry1Ai Toxin is Located Between 36I and 605I |
| title_short | The Minimal Active Fragment of the Cry1Ai Toxin is Located Between 36I and 605I |
| title_sort | minimal active fragment of the cry1ai toxin is located between 36i and 605i |
| topic | Bacillus thuringiensis Cry1Ai toxin active fragment toxicity Plutella xylostella |
| url | http://www.sciencedirect.com/science/article/pii/S2095311913605328 |
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