Glycosylation Profile of the Transferrin Receptor in Gestational Iron Deficiency and Early-Onset Severe Preeclampsia
Objective. To examine the expression of hypoxia-inducible factor-1α (HIF-1α), TfR1, and TfR1-attached terminal monosaccharides in placentas of women with IDAP and severe preeclampsia. Methods. TfR1 and HIF-1α were detected by western blot. Immunoadsorption of TfR1 was performed to characterize the t...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Journal of Pregnancy |
| Online Access: | http://dx.doi.org/10.1155/2019/9514546 |
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| author | Alejandra María Gómez-Gutiérrez Beatriz Elena Parra-Sosa Julio Cesar Bueno-Sánchez |
| author_facet | Alejandra María Gómez-Gutiérrez Beatriz Elena Parra-Sosa Julio Cesar Bueno-Sánchez |
| author_sort | Alejandra María Gómez-Gutiérrez |
| collection | DOAJ |
| description | Objective. To examine the expression of hypoxia-inducible factor-1α (HIF-1α), TfR1, and TfR1-attached terminal monosaccharides in placentas of women with IDAP and severe preeclampsia. Methods. TfR1 and HIF-1α were detected by western blot. Immunoadsorption of TfR1 was performed to characterize the terminal monosaccharides by specific lectin binding. Results. There was no difference in the expression of TfR1 and HIF-1α between groups. Lectin blot analysis pointed out an overexpression of galactose β1-4 N-acetylglucosamine (Gal-GlcNAc) and mannose in severe preeclampsia. Conclusion. The increase in Gal-GlcNAc may be due to the increased presence of antennary structures and the mannose glycans of TfR1 may indicate the presence of misfolded or incomplete proteins. These findings may be associated with the low expression of placental TfR1 in women with preeclampsia. |
| format | Article |
| id | doaj-art-d3927389251c47dfa8867fd7a4e4e1f8 |
| institution | Kabale University |
| issn | 2090-2727 2090-2735 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Pregnancy |
| spelling | doaj-art-d3927389251c47dfa8867fd7a4e4e1f82025-02-03T01:02:49ZengWileyJournal of Pregnancy2090-27272090-27352019-01-01201910.1155/2019/95145469514546Glycosylation Profile of the Transferrin Receptor in Gestational Iron Deficiency and Early-Onset Severe PreeclampsiaAlejandra María Gómez-Gutiérrez0Beatriz Elena Parra-Sosa1Julio Cesar Bueno-Sánchez2Professor Facultad de Medicina, Departamento de Fisiología y Bioquímica, Grupo Reproducción, Universidad de Antioquia, Carrera 53 # 61-30, Torre 2, Laboratorio 534, Medellín, ColombiaGrupo de Investigación Alimentación y Nutrición Humana (GIANH-END-UdeA), Universidad de Antioquia, Calle 62 No 52-59, Torre 1, Laboratorio 413, Medellín, ColombiaProfessor Facultad de Medicina, Departamento de Fisiología y Bioquímica, Grupo Reproducción, Universidad de Antioquia, Carrera 53 # 61-30, Torre 2, Laboratorio 534, Medellín, ColombiaObjective. To examine the expression of hypoxia-inducible factor-1α (HIF-1α), TfR1, and TfR1-attached terminal monosaccharides in placentas of women with IDAP and severe preeclampsia. Methods. TfR1 and HIF-1α were detected by western blot. Immunoadsorption of TfR1 was performed to characterize the terminal monosaccharides by specific lectin binding. Results. There was no difference in the expression of TfR1 and HIF-1α between groups. Lectin blot analysis pointed out an overexpression of galactose β1-4 N-acetylglucosamine (Gal-GlcNAc) and mannose in severe preeclampsia. Conclusion. The increase in Gal-GlcNAc may be due to the increased presence of antennary structures and the mannose glycans of TfR1 may indicate the presence of misfolded or incomplete proteins. These findings may be associated with the low expression of placental TfR1 in women with preeclampsia.http://dx.doi.org/10.1155/2019/9514546 |
| spellingShingle | Alejandra María Gómez-Gutiérrez Beatriz Elena Parra-Sosa Julio Cesar Bueno-Sánchez Glycosylation Profile of the Transferrin Receptor in Gestational Iron Deficiency and Early-Onset Severe Preeclampsia Journal of Pregnancy |
| title | Glycosylation Profile of the Transferrin Receptor in Gestational Iron Deficiency and Early-Onset Severe Preeclampsia |
| title_full | Glycosylation Profile of the Transferrin Receptor in Gestational Iron Deficiency and Early-Onset Severe Preeclampsia |
| title_fullStr | Glycosylation Profile of the Transferrin Receptor in Gestational Iron Deficiency and Early-Onset Severe Preeclampsia |
| title_full_unstemmed | Glycosylation Profile of the Transferrin Receptor in Gestational Iron Deficiency and Early-Onset Severe Preeclampsia |
| title_short | Glycosylation Profile of the Transferrin Receptor in Gestational Iron Deficiency and Early-Onset Severe Preeclampsia |
| title_sort | glycosylation profile of the transferrin receptor in gestational iron deficiency and early onset severe preeclampsia |
| url | http://dx.doi.org/10.1155/2019/9514546 |
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