The importance of blood culture and serology for Brucellosis diagnosis and treatment
Introduction: Brucellosis is a zoonotic endemic disease in Lebanon. It is caused by direct transmission of Brucella from contaminated animal products to humans. If left untreated brucellosis might lead to several complications and a chronic disease state. The prompt diagnosis of brucellosis has the...
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The Journal of Infection in Developing Countries
2018-02-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/10120 |
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| author | Lama Faddoul Rania Azar Antoine Haddad Abi Hanna |
| author_facet | Lama Faddoul Rania Azar Antoine Haddad Abi Hanna |
| author_sort | Lama Faddoul |
| collection | DOAJ |
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Introduction: Brucellosis is a zoonotic endemic disease in Lebanon. It is caused by direct transmission of Brucella from contaminated animal products to humans. If left untreated brucellosis might lead to several complications and a chronic disease state. The prompt diagnosis of brucellosis has the ability to limit the progression of the disease, especially if the correct treatment is administered for the adequate amount of time. The aim of this study is to determine the optimal diagnostic tool and to assess Brucella burden in Lebanon.
Methodology: This retrospective study was performed by reviewing the medical charts of 46 brucellosis patients from three Lebanese hospitals. Brucellosis diagnostic tests were compared and sensitivity of each test was calculated, as well as, the level of agreement with other standard diagnostic tools. Data retrieved were analyzed for relevance and statistical significance using the statistical package for social sciences version 23.
Results: Sensitivity results of the diagnostic tests were: Rose Bengal test (RBT) 94.7%, blood culture 65.6%, standard agglutination test (SAT) melitensis 95.1% and SAT abortus 97.6%. The level of agreement between RBT and SAT melitensis as well as abortus is 98% and 90.18%, respectively. While the level of agreement between Blood culture and SAT melitensis as well as abortus is 66.88% and 64.5%, respectively.
Discussion: Culture techniques require further optimization in order to find the best diagnostic tool for brucellosis. Meanwhile, Blood Rose Bengal test held a significant potential for identifying Brucella infection in a highly sensitive, cost effective and time saving manner.
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| format | Article |
| id | doaj-art-d38f182d91244de7a39f9efd8531ac45 |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2018-02-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-d38f182d91244de7a39f9efd8531ac452025-08-20T03:52:42ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802018-02-011202.110.3855/jidc.10120The importance of blood culture and serology for Brucellosis diagnosis and treatmentLama Faddoul0Rania Azar1Antoine Haddad2Abi Hanna3Lebanese International University, Beirut, LebanonLebanese University, Beirut, LebanonLebanese University, Beirut, LebanonLebanese University, Beirut, Lebanon Introduction: Brucellosis is a zoonotic endemic disease in Lebanon. It is caused by direct transmission of Brucella from contaminated animal products to humans. If left untreated brucellosis might lead to several complications and a chronic disease state. The prompt diagnosis of brucellosis has the ability to limit the progression of the disease, especially if the correct treatment is administered for the adequate amount of time. The aim of this study is to determine the optimal diagnostic tool and to assess Brucella burden in Lebanon. Methodology: This retrospective study was performed by reviewing the medical charts of 46 brucellosis patients from three Lebanese hospitals. Brucellosis diagnostic tests were compared and sensitivity of each test was calculated, as well as, the level of agreement with other standard diagnostic tools. Data retrieved were analyzed for relevance and statistical significance using the statistical package for social sciences version 23. Results: Sensitivity results of the diagnostic tests were: Rose Bengal test (RBT) 94.7%, blood culture 65.6%, standard agglutination test (SAT) melitensis 95.1% and SAT abortus 97.6%. The level of agreement between RBT and SAT melitensis as well as abortus is 98% and 90.18%, respectively. While the level of agreement between Blood culture and SAT melitensis as well as abortus is 66.88% and 64.5%, respectively. Discussion: Culture techniques require further optimization in order to find the best diagnostic tool for brucellosis. Meanwhile, Blood Rose Bengal test held a significant potential for identifying Brucella infection in a highly sensitive, cost effective and time saving manner. https://jidc.org/index.php/journal/article/view/10120Brucellamelitensisabortusdiagnostic testrose bengal testblood culture |
| spellingShingle | Lama Faddoul Rania Azar Antoine Haddad Abi Hanna The importance of blood culture and serology for Brucellosis diagnosis and treatment Journal of Infection in Developing Countries Brucella melitensis abortus diagnostic test rose bengal test blood culture |
| title | The importance of blood culture and serology for Brucellosis diagnosis and treatment |
| title_full | The importance of blood culture and serology for Brucellosis diagnosis and treatment |
| title_fullStr | The importance of blood culture and serology for Brucellosis diagnosis and treatment |
| title_full_unstemmed | The importance of blood culture and serology for Brucellosis diagnosis and treatment |
| title_short | The importance of blood culture and serology for Brucellosis diagnosis and treatment |
| title_sort | importance of blood culture and serology for brucellosis diagnosis and treatment |
| topic | Brucella melitensis abortus diagnostic test rose bengal test blood culture |
| url | https://jidc.org/index.php/journal/article/view/10120 |
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