Detection and isolation of a new member of Burkholderiaceae-related endofungal bacteria from Saksenaea boninensis sp. nov., a new thermotolerant fungus in Mucorales
Abstract Thermotolerance in Mucorales (Mucoromycotina) is one of the factors to be opportunistic pathogens, causing mucormycosis. Among thermotolerant mucoralean fungi, Burkholderiaceae-related endobacteria (BRE) are rarely found and the known range of hosts is limited to Rhizopus spp. The phylogene...
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2023-11-01
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| Series: | IMA Fungus |
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| Online Access: | https://doi.org/10.1186/s43008-023-00129-2 |
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| author | Yusuke Takashima Kohei Yamamoto Yousuke Degawa Yong Guo Tomoyasu Nishizawa Hiroyuki Ohta Kazuhiko Narisawa |
| author_facet | Yusuke Takashima Kohei Yamamoto Yousuke Degawa Yong Guo Tomoyasu Nishizawa Hiroyuki Ohta Kazuhiko Narisawa |
| author_sort | Yusuke Takashima |
| collection | DOAJ |
| description | Abstract Thermotolerance in Mucorales (Mucoromycotina) is one of the factors to be opportunistic pathogens, causing mucormycosis. Among thermotolerant mucoralean fungi, Burkholderiaceae-related endobacteria (BRE) are rarely found and the known range of hosts is limited to Rhizopus spp. The phylogenetic divergence of BRE has recently expanded in other fungal groups such as Mortierellaceae spp. (Mortierellomycotina); however, it remains unexplored in Mucorales. Here, we found a thermotolerant mucoralean fungus obtained from a litter sample collected from Haha-jima Island in the Ogasawara (Bonin) Islands, Japan. The fungus was morphologically, phylogenetically, and physiologically characterized and proposed as a new species, Saksenaea boninensis sp. nov. Besides the fungal taxonomy, we also found the presence of BRE in isolates of this species by diagnostic PCR amplification of the 16S rRNA gene from mycelia, fluorescence microscopic observations, and isolation of the bacterium in pure culture. Phylogenetic analysis of the 16S rRNA gene of BRE revealed that it is distinct from all known BRE. The discovery of a culturable BRE lineage in the genus Saksenaea will add new insight into the evolutional origin of mucoralean fungus-BRE associations and emphasize the need to pay more attention to endofungal bacteria potentially associated with isolates of thermotolerant mucoralean fungi causing mucormycosis. |
| format | Article |
| id | doaj-art-d3853626943c49b89879067eeb66e250 |
| institution | Kabale University |
| issn | 2210-6359 |
| language | English |
| publishDate | 2023-11-01 |
| publisher | BMC |
| record_format | Article |
| series | IMA Fungus |
| spelling | doaj-art-d3853626943c49b89879067eeb66e2502025-02-03T09:36:04ZengBMCIMA Fungus2210-63592023-11-0114111710.1186/s43008-023-00129-2Detection and isolation of a new member of Burkholderiaceae-related endofungal bacteria from Saksenaea boninensis sp. nov., a new thermotolerant fungus in MucoralesYusuke Takashima0Kohei Yamamoto1Yousuke Degawa2Yong Guo3Tomoyasu Nishizawa4Hiroyuki Ohta5Kazuhiko Narisawa6Genetic Resources Center, National Agriculture and Food Research OrganizationTochigi Prefectural MuseumMountain Science Center, Sugadaira Research Station, University of TsukubaIbaraki University College of AgricultureIbaraki University College of AgricultureIbaraki UniversityIbaraki University College of AgricultureAbstract Thermotolerance in Mucorales (Mucoromycotina) is one of the factors to be opportunistic pathogens, causing mucormycosis. Among thermotolerant mucoralean fungi, Burkholderiaceae-related endobacteria (BRE) are rarely found and the known range of hosts is limited to Rhizopus spp. The phylogenetic divergence of BRE has recently expanded in other fungal groups such as Mortierellaceae spp. (Mortierellomycotina); however, it remains unexplored in Mucorales. Here, we found a thermotolerant mucoralean fungus obtained from a litter sample collected from Haha-jima Island in the Ogasawara (Bonin) Islands, Japan. The fungus was morphologically, phylogenetically, and physiologically characterized and proposed as a new species, Saksenaea boninensis sp. nov. Besides the fungal taxonomy, we also found the presence of BRE in isolates of this species by diagnostic PCR amplification of the 16S rRNA gene from mycelia, fluorescence microscopic observations, and isolation of the bacterium in pure culture. Phylogenetic analysis of the 16S rRNA gene of BRE revealed that it is distinct from all known BRE. The discovery of a culturable BRE lineage in the genus Saksenaea will add new insight into the evolutional origin of mucoralean fungus-BRE associations and emphasize the need to pay more attention to endofungal bacteria potentially associated with isolates of thermotolerant mucoralean fungi causing mucormycosis.https://doi.org/10.1186/s43008-023-00129-2Endohyphal bacteriaMucormycosisMucoromycotinaOne new taxonOpportunistic pathogensSaksenaeaceae |
| spellingShingle | Yusuke Takashima Kohei Yamamoto Yousuke Degawa Yong Guo Tomoyasu Nishizawa Hiroyuki Ohta Kazuhiko Narisawa Detection and isolation of a new member of Burkholderiaceae-related endofungal bacteria from Saksenaea boninensis sp. nov., a new thermotolerant fungus in Mucorales IMA Fungus Endohyphal bacteria Mucormycosis Mucoromycotina One new taxon Opportunistic pathogens Saksenaeaceae |
| title | Detection and isolation of a new member of Burkholderiaceae-related endofungal bacteria from Saksenaea boninensis sp. nov., a new thermotolerant fungus in Mucorales |
| title_full | Detection and isolation of a new member of Burkholderiaceae-related endofungal bacteria from Saksenaea boninensis sp. nov., a new thermotolerant fungus in Mucorales |
| title_fullStr | Detection and isolation of a new member of Burkholderiaceae-related endofungal bacteria from Saksenaea boninensis sp. nov., a new thermotolerant fungus in Mucorales |
| title_full_unstemmed | Detection and isolation of a new member of Burkholderiaceae-related endofungal bacteria from Saksenaea boninensis sp. nov., a new thermotolerant fungus in Mucorales |
| title_short | Detection and isolation of a new member of Burkholderiaceae-related endofungal bacteria from Saksenaea boninensis sp. nov., a new thermotolerant fungus in Mucorales |
| title_sort | detection and isolation of a new member of burkholderiaceae related endofungal bacteria from saksenaea boninensis sp nov a new thermotolerant fungus in mucorales |
| topic | Endohyphal bacteria Mucormycosis Mucoromycotina One new taxon Opportunistic pathogens Saksenaeaceae |
| url | https://doi.org/10.1186/s43008-023-00129-2 |
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