Transcriptomic profiling reveals response mechanisms of Lactuca indica seedlings to seawater irrigation stress
IntroductionThe increasing global soil salinization has accelerated research on seawater irrigation agriculture. Developing wild vegetables through seawater irrigation could establish foundational strategies for utilizing island vegetable germplasm resources.MethodsThis study investigated two distin...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-06-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2025.1599564/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850212493230604288 |
|---|---|
| author | Dingding Cao Lirong Xiang Ziling Li Neng Wei Neng Wei Qingfeng Wang Qingfeng Wang |
| author_facet | Dingding Cao Lirong Xiang Ziling Li Neng Wei Neng Wei Qingfeng Wang Qingfeng Wang |
| author_sort | Dingding Cao |
| collection | DOAJ |
| description | IntroductionThe increasing global soil salinization has accelerated research on seawater irrigation agriculture. Developing wild vegetables through seawater irrigation could establish foundational strategies for utilizing island vegetable germplasm resources.MethodsThis study investigated two distinct leaf-shaped individuals (S and Y) of wild Lactuca indica (L. indica) through hydroponic experiments with diluted seawater during seedling stage. Physiological and morphological assessments revealed that Y exhibited superior seawater tolerance compared to S. Tissue-specific -plant transcriptome analysis identified key metabolic pathways and regulatory genes in roots, stems, and leaves.ResultsDifferential gene expression analysis showed tissue-specific enrichment patterns: leaves predominantly enriched light-harvesting complex (LHC) genes in photosynthesis pathways; stems exhibited upregulation in cutin, suberin, and wax biosynthesis pathways; while roots showed activation of nitrogen metabolism pathways.DiscussionBased on the data from transcriptomics, we infered that the key salt-tolerant candidate genes include: (1) leaf-specific LHC genes enhancing photosynthetic efficiency; (2) stem-expressed wax biosynthesis gene aldehyde decarbonylase CER1, and cytochrome P450 family members fatty acid omega-hydroxylase CYP86A4S and cytochrome P450 family 77 subfamily A (CYP77A); and (3) root-specific nitrogen metabolism regulators (nitrate reductase (NR), nitrate/nitrite transporter 2 (NRT2), and nitrite reductase (NirA). This study provides the comprehensive tissue-specific transcriptome profile of wild L. indica under seawater irrigation, predicting crucial metabolic pathways and candidate genes that might enhance seawater tolerance. Our findings establish a valuable reference for salt tolerance research in wild vegetables and offer potential genetic targets for improving crop resilience in saline-affected ecosystems. |
| format | Article |
| id | doaj-art-d381b4e7e38440f3825005cab5978e93 |
| institution | OA Journals |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-d381b4e7e38440f3825005cab5978e932025-08-20T02:09:19ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-06-011610.3389/fpls.2025.15995641599564Transcriptomic profiling reveals response mechanisms of Lactuca indica seedlings to seawater irrigation stressDingding Cao0Lirong Xiang1Ziling Li2Neng Wei3Neng Wei4Qingfeng Wang5Qingfeng Wang6Fujian Key Laboratory on Conservation and Sustainable Utilization of Marine Biodiversity, Fuzhou Institute of Oceanography, College of Geography and Oceanography, Minjiang University, Fuzhou, ChinaCollege of Life Sciences, Hubei University, Wuhan, ChinaFujian Key Laboratory on Conservation and Sustainable Utilization of Marine Biodiversity, Fuzhou Institute of Oceanography, College of Geography and Oceanography, Minjiang University, Fuzhou, ChinaState Key Laboratory of Plant Diversity and Specialty Crops, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, ChinaSino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan, ChinaState Key Laboratory of Plant Diversity and Specialty Crops, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, ChinaSino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan, ChinaIntroductionThe increasing global soil salinization has accelerated research on seawater irrigation agriculture. Developing wild vegetables through seawater irrigation could establish foundational strategies for utilizing island vegetable germplasm resources.MethodsThis study investigated two distinct leaf-shaped individuals (S and Y) of wild Lactuca indica (L. indica) through hydroponic experiments with diluted seawater during seedling stage. Physiological and morphological assessments revealed that Y exhibited superior seawater tolerance compared to S. Tissue-specific -plant transcriptome analysis identified key metabolic pathways and regulatory genes in roots, stems, and leaves.ResultsDifferential gene expression analysis showed tissue-specific enrichment patterns: leaves predominantly enriched light-harvesting complex (LHC) genes in photosynthesis pathways; stems exhibited upregulation in cutin, suberin, and wax biosynthesis pathways; while roots showed activation of nitrogen metabolism pathways.DiscussionBased on the data from transcriptomics, we infered that the key salt-tolerant candidate genes include: (1) leaf-specific LHC genes enhancing photosynthetic efficiency; (2) stem-expressed wax biosynthesis gene aldehyde decarbonylase CER1, and cytochrome P450 family members fatty acid omega-hydroxylase CYP86A4S and cytochrome P450 family 77 subfamily A (CYP77A); and (3) root-specific nitrogen metabolism regulators (nitrate reductase (NR), nitrate/nitrite transporter 2 (NRT2), and nitrite reductase (NirA). This study provides the comprehensive tissue-specific transcriptome profile of wild L. indica under seawater irrigation, predicting crucial metabolic pathways and candidate genes that might enhance seawater tolerance. Our findings establish a valuable reference for salt tolerance research in wild vegetables and offer potential genetic targets for improving crop resilience in saline-affected ecosystems.https://www.frontiersin.org/articles/10.3389/fpls.2025.1599564/fulldifferential gene expressionsalt toleranceseawater irrigationtranscriptome analysiswild vegetables |
| spellingShingle | Dingding Cao Lirong Xiang Ziling Li Neng Wei Neng Wei Qingfeng Wang Qingfeng Wang Transcriptomic profiling reveals response mechanisms of Lactuca indica seedlings to seawater irrigation stress Frontiers in Plant Science differential gene expression salt tolerance seawater irrigation transcriptome analysis wild vegetables |
| title | Transcriptomic profiling reveals response mechanisms of Lactuca indica seedlings to seawater irrigation stress |
| title_full | Transcriptomic profiling reveals response mechanisms of Lactuca indica seedlings to seawater irrigation stress |
| title_fullStr | Transcriptomic profiling reveals response mechanisms of Lactuca indica seedlings to seawater irrigation stress |
| title_full_unstemmed | Transcriptomic profiling reveals response mechanisms of Lactuca indica seedlings to seawater irrigation stress |
| title_short | Transcriptomic profiling reveals response mechanisms of Lactuca indica seedlings to seawater irrigation stress |
| title_sort | transcriptomic profiling reveals response mechanisms of lactuca indica seedlings to seawater irrigation stress |
| topic | differential gene expression salt tolerance seawater irrigation transcriptome analysis wild vegetables |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2025.1599564/full |
| work_keys_str_mv | AT dingdingcao transcriptomicprofilingrevealsresponsemechanismsoflactucaindicaseedlingstoseawaterirrigationstress AT lirongxiang transcriptomicprofilingrevealsresponsemechanismsoflactucaindicaseedlingstoseawaterirrigationstress AT zilingli transcriptomicprofilingrevealsresponsemechanismsoflactucaindicaseedlingstoseawaterirrigationstress AT nengwei transcriptomicprofilingrevealsresponsemechanismsoflactucaindicaseedlingstoseawaterirrigationstress AT nengwei transcriptomicprofilingrevealsresponsemechanismsoflactucaindicaseedlingstoseawaterirrigationstress AT qingfengwang transcriptomicprofilingrevealsresponsemechanismsoflactucaindicaseedlingstoseawaterirrigationstress AT qingfengwang transcriptomicprofilingrevealsresponsemechanismsoflactucaindicaseedlingstoseawaterirrigationstress |