Isolation and characterization of Salmonella Enteritidis and Salmonella Typhimurium from chicken meat in Egypt
Introduction: Salmonella enterica serovars Enteritidis and Typhimurium represent the major serovars associated with human salmonellosis. Contamination of meat products with these serovars is considered the main source of infection. Methodology: In this study, 100 raw chicken meat samples were inves...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Journal of Infection in Developing Countries
2017-04-01
|
| Series: | Journal of Infection in Developing Countries |
| Subjects: | |
| Online Access: | https://jidc.org/index.php/journal/article/view/8043 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850193886450810880 |
|---|---|
| author | Reda Tarabees Mohamed S. A. Elsayed Reyad Shawish Shereen Basiouni Awad A Shehata |
| author_facet | Reda Tarabees Mohamed S. A. Elsayed Reyad Shawish Shereen Basiouni Awad A Shehata |
| author_sort | Reda Tarabees |
| collection | DOAJ |
| description | Introduction: Salmonella enterica serovars Enteritidis and Typhimurium represent the major serovars associated with human salmonellosis. Contamination of meat products with these serovars is considered the main source of infection.
Methodology: In this study, 100 raw chicken meat samples were investigated for the presence of Salmonella spp., which were subsequently identified based on biochemical and serological tests as well as matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) profile. Furthermore, the isolated serovars were examined using multiplex polymerase chain reaction (PCR) for the presence of virulence genes suspected to have a role in infection.
Results: S. Enteritidis was isolated from two samples (2%), while S. Typhimurium was isolated from three samples (3%) of chicken meat. Of the 17 examined virulence genes using multiplex PCR, the sitC, sopB, sifA, lpfC, spaN, sipB, invA, spiA, and msgA genes were detected in S. Enteritidis. However, the sitC, iroN, sopB, sifA, lpfC, spaN, sipB, invA, and tolC genes were successfully amplified in S. Typhimurium.
Conclusions: The detection of S. Enteritidis and S. Typhimurium in meat, even at low incidence, has important implications. In addition, the data presented here is the first attempt to identify a wide range of virulence genes in Egyptian Salmonella isolates recovered from meat products. A strict public health and food safety regime is urgently needed in order to decrease the human health hazard risk associated with salmonellosis.
|
| format | Article |
| id | doaj-art-d375dc8c64974a10a30db85b4ea01e6a |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2017-04-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-d375dc8c64974a10a30db85b4ea01e6a2025-08-20T02:14:07ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802017-04-01110410.3855/jidc.8043Isolation and characterization of Salmonella Enteritidis and Salmonella Typhimurium from chicken meat in EgyptReda Tarabees0Mohamed S. A. Elsayed1Reyad Shawish2Shereen Basiouni3Awad A Shehata4Faculty of Veterinary Medicine, El Sadat City University, Sadat City, Minoufiya, EgyptFaculty of Veterinary Medicine, El Sadat City University, Sadat City, Minoufiya, EgyptFaculty of Veterinary Medicine, Sadat City University, Minoufiya, EgyptFaculty of Veterinary Medicine, Benha University, Qalioubeya, EgyptFaculty of Veterinary Medicine, Sadat City University, EgyptIntroduction: Salmonella enterica serovars Enteritidis and Typhimurium represent the major serovars associated with human salmonellosis. Contamination of meat products with these serovars is considered the main source of infection. Methodology: In this study, 100 raw chicken meat samples were investigated for the presence of Salmonella spp., which were subsequently identified based on biochemical and serological tests as well as matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) profile. Furthermore, the isolated serovars were examined using multiplex polymerase chain reaction (PCR) for the presence of virulence genes suspected to have a role in infection. Results: S. Enteritidis was isolated from two samples (2%), while S. Typhimurium was isolated from three samples (3%) of chicken meat. Of the 17 examined virulence genes using multiplex PCR, the sitC, sopB, sifA, lpfC, spaN, sipB, invA, spiA, and msgA genes were detected in S. Enteritidis. However, the sitC, iroN, sopB, sifA, lpfC, spaN, sipB, invA, and tolC genes were successfully amplified in S. Typhimurium. Conclusions: The detection of S. Enteritidis and S. Typhimurium in meat, even at low incidence, has important implications. In addition, the data presented here is the first attempt to identify a wide range of virulence genes in Egyptian Salmonella isolates recovered from meat products. A strict public health and food safety regime is urgently needed in order to decrease the human health hazard risk associated with salmonellosis. https://jidc.org/index.php/journal/article/view/8043Salmonellavirulence genesmultiplex PCRMALDI-TOF |
| spellingShingle | Reda Tarabees Mohamed S. A. Elsayed Reyad Shawish Shereen Basiouni Awad A Shehata Isolation and characterization of Salmonella Enteritidis and Salmonella Typhimurium from chicken meat in Egypt Journal of Infection in Developing Countries Salmonella virulence genes multiplex PCR MALDI-TOF |
| title | Isolation and characterization of Salmonella Enteritidis and Salmonella Typhimurium from chicken meat in Egypt |
| title_full | Isolation and characterization of Salmonella Enteritidis and Salmonella Typhimurium from chicken meat in Egypt |
| title_fullStr | Isolation and characterization of Salmonella Enteritidis and Salmonella Typhimurium from chicken meat in Egypt |
| title_full_unstemmed | Isolation and characterization of Salmonella Enteritidis and Salmonella Typhimurium from chicken meat in Egypt |
| title_short | Isolation and characterization of Salmonella Enteritidis and Salmonella Typhimurium from chicken meat in Egypt |
| title_sort | isolation and characterization of salmonella enteritidis and salmonella typhimurium from chicken meat in egypt |
| topic | Salmonella virulence genes multiplex PCR MALDI-TOF |
| url | https://jidc.org/index.php/journal/article/view/8043 |
| work_keys_str_mv | AT redatarabees isolationandcharacterizationofsalmonellaenteritidisandsalmonellatyphimuriumfromchickenmeatinegypt AT mohamedsaelsayed isolationandcharacterizationofsalmonellaenteritidisandsalmonellatyphimuriumfromchickenmeatinegypt AT reyadshawish isolationandcharacterizationofsalmonellaenteritidisandsalmonellatyphimuriumfromchickenmeatinegypt AT shereenbasiouni isolationandcharacterizationofsalmonellaenteritidisandsalmonellatyphimuriumfromchickenmeatinegypt AT awadashehata isolationandcharacterizationofsalmonellaenteritidisandsalmonellatyphimuriumfromchickenmeatinegypt |