Evaluation of interaction among arsenic and Brevibacterium sp. strain CS2 and its proteins profiling
In this study, Brevibacterium sp. strain CS2 was used to evaluate the mechanisms of arsenic interaction with the bacterium and its enzymatic and protein profiling under arsenic stress. The bacterium was capable to resist the arsenate 280 mM and arsenite 40 mM as per MIC. The whole genome, available...
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| Language: | English |
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Elsevier
2024-11-01
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| Series: | Journal of Hazardous Materials Letters |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666911024000182 |
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| author | Shahid Sher Sajjad Ullah Dilara Abbas Bukhari Syed Zajif Hussain Abdul Rehman |
| author_facet | Shahid Sher Sajjad Ullah Dilara Abbas Bukhari Syed Zajif Hussain Abdul Rehman |
| author_sort | Shahid Sher |
| collection | DOAJ |
| description | In this study, Brevibacterium sp. strain CS2 was used to evaluate the mechanisms of arsenic interaction with the bacterium and its enzymatic and protein profiling under arsenic stress. The bacterium was capable to resist the arsenate 280 mM and arsenite 40 mM as per MIC. The whole genome, available on NCBI, was analyzed for genes associated with arsenic, which confirmed the genes for both arsenic oxidation (aioB) and arsenic reduction arsR, arsC, ACR3, and arsB. The sharpening and shifting of FTIR spectra in the ranges of 3278–2851 cm−1 are due to hydroxyl and amide stretching. SEM analysis showed no significant changes in morphology in arsenic stress while EDX analysis proved the arsenite interaction by showing arsenic peaks in the graph. Both glutathione and non-protein thiol showed different responses in the absence and presence of arsenic stress. Protein bands such as 25, 30, 32, 37, 42, 48, and 100 kDa were expressed more in arsenic-treated samples as compared to the control one. The presence of arsenic oxidizing genes, the ability to resist arsenic, and the varied response of enzymes and proteins in arsenic stress make the bacterium a suitable agent for arsenic eradication from contaminated sites. |
| format | Article |
| id | doaj-art-d368bedcd7e042f19614fe89c24c53e7 |
| institution | OA Journals |
| issn | 2666-9110 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Hazardous Materials Letters |
| spelling | doaj-art-d368bedcd7e042f19614fe89c24c53e72025-08-20T02:32:45ZengElsevierJournal of Hazardous Materials Letters2666-91102024-11-01510011910.1016/j.hazl.2024.100119Evaluation of interaction among arsenic and Brevibacterium sp. strain CS2 and its proteins profilingShahid Sher0Sajjad Ullah1Dilara Abbas Bukhari2Syed Zajif Hussain3Abdul Rehman4University Institute of Medical Laboratory Technology (UIMLT), Faculty of Allied Health Sciences (FAHS), The University of Lahore, Lahore, Pakistan; School of Environment Florida Agricultural and Mechanical University, United States of AmericaUniversity Institute of Medical Laboratory Technology (UIMLT), Faculty of Allied Health Sciences (FAHS), The University of Lahore, Lahore, PakistanDepartment of Zoology, Government College University, Lahore, PakistanDepartment of Chemistry, SBA School of Science and Engineering (SBASSE), Lahore University of Management Sciences (LUMS), DHA, Lahore Cantt, 54792, PakistanInstitute of Microbiology and Molecular Genetics, University of the Punjab, New Campus, Lahore 54590, Pakistan; Correspondence to: Department of Microbiology & Molecular Genetics, University of the Punjab, New Campus, Lahore 54590, Pakistan.In this study, Brevibacterium sp. strain CS2 was used to evaluate the mechanisms of arsenic interaction with the bacterium and its enzymatic and protein profiling under arsenic stress. The bacterium was capable to resist the arsenate 280 mM and arsenite 40 mM as per MIC. The whole genome, available on NCBI, was analyzed for genes associated with arsenic, which confirmed the genes for both arsenic oxidation (aioB) and arsenic reduction arsR, arsC, ACR3, and arsB. The sharpening and shifting of FTIR spectra in the ranges of 3278–2851 cm−1 are due to hydroxyl and amide stretching. SEM analysis showed no significant changes in morphology in arsenic stress while EDX analysis proved the arsenite interaction by showing arsenic peaks in the graph. Both glutathione and non-protein thiol showed different responses in the absence and presence of arsenic stress. Protein bands such as 25, 30, 32, 37, 42, 48, and 100 kDa were expressed more in arsenic-treated samples as compared to the control one. The presence of arsenic oxidizing genes, the ability to resist arsenic, and the varied response of enzymes and proteins in arsenic stress make the bacterium a suitable agent for arsenic eradication from contaminated sites.http://www.sciencedirect.com/science/article/pii/S2666911024000182ArsenicBrevibacterium sp. strain CS2FTIRSEMEDXSDS-PAGE |
| spellingShingle | Shahid Sher Sajjad Ullah Dilara Abbas Bukhari Syed Zajif Hussain Abdul Rehman Evaluation of interaction among arsenic and Brevibacterium sp. strain CS2 and its proteins profiling Journal of Hazardous Materials Letters Arsenic Brevibacterium sp. strain CS2 FTIR SEM EDX SDS-PAGE |
| title | Evaluation of interaction among arsenic and Brevibacterium sp. strain CS2 and its proteins profiling |
| title_full | Evaluation of interaction among arsenic and Brevibacterium sp. strain CS2 and its proteins profiling |
| title_fullStr | Evaluation of interaction among arsenic and Brevibacterium sp. strain CS2 and its proteins profiling |
| title_full_unstemmed | Evaluation of interaction among arsenic and Brevibacterium sp. strain CS2 and its proteins profiling |
| title_short | Evaluation of interaction among arsenic and Brevibacterium sp. strain CS2 and its proteins profiling |
| title_sort | evaluation of interaction among arsenic and brevibacterium sp strain cs2 and its proteins profiling |
| topic | Arsenic Brevibacterium sp. strain CS2 FTIR SEM EDX SDS-PAGE |
| url | http://www.sciencedirect.com/science/article/pii/S2666911024000182 |
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