Influence of Plant-Based Substrate Composition and Extraction Method on Accumulation of Bioactive Compounds in <i>Hericium erinaceus</i> (Bull.) Pers. Fruiting Bodies
The selection of plant-based substrates for mushroom cultivation is a key factor influencing their growth and metabolism. The aim of this study was to demonstrate, in an innovative approach, differences in the content of biologically active compounds, bioelements, and antioxidant properties of <i...
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2025-07-01
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| author | Katarzyna Kała Małgorzata Cicha-Jeleń Katarzyna Sułkowska-Ziaja Beata Ostachowicz Ewa Węgrzynowicz Jan Lazur Agnieszka Szewczyk Bożena Muszyńska |
| author_facet | Katarzyna Kała Małgorzata Cicha-Jeleń Katarzyna Sułkowska-Ziaja Beata Ostachowicz Ewa Węgrzynowicz Jan Lazur Agnieszka Szewczyk Bożena Muszyńska |
| author_sort | Katarzyna Kała |
| collection | DOAJ |
| description | The selection of plant-based substrates for mushroom cultivation is a key factor influencing their growth and metabolism. The aim of this study was to demonstrate, in an innovative approach, differences in the content of biologically active compounds, bioelements, and antioxidant properties of <i>Hericium erinaceus</i> (Bull.) Pers. cultivated on various plant-based substrates derived from waste materials, specifically hemp straw and beech sawdust. Another objective was to compare various extraction methods in terms of their impact on the concentration of these compounds. Elemental analysis was performed using the TXRF method, while bioactive constituents were determined using the DAD/UV RP-HPLC technique. The plant-based substrate and extraction method influenced the levels of obtained metabolites. Dual extraction with moderate ethanol concentrations was most effective for isolating key bioactive compounds from <i>H. erinaceus</i>—notably ergothioneine, lovastatin, L-phenylalanine, and ergosterol—while antioxidant activity did not correlate with the concentration of the solvent used. Although dual extracts enhanced certain antioxidants and metabolites, whole fruiting bodies contained higher levels of bioelements. Overall, fruiting bodies grown on beech sawdust had greater amounts of most bioactive compounds compared to those cultivated on hemp straw, emphasizing that both substrate choice and extraction method critically influence the mushroom’s bioactive profile and its potential health benefits. |
| format | Article |
| id | doaj-art-d36887b48f814148b004d2b1d2252ed2 |
| institution | Kabale University |
| issn | 1420-3049 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj-art-d36887b48f814148b004d2b1d2252ed22025-08-20T03:36:36ZengMDPI AGMolecules1420-30492025-07-013015309410.3390/molecules30153094Influence of Plant-Based Substrate Composition and Extraction Method on Accumulation of Bioactive Compounds in <i>Hericium erinaceus</i> (Bull.) Pers. Fruiting BodiesKatarzyna Kała0Małgorzata Cicha-Jeleń1Katarzyna Sułkowska-Ziaja2Beata Ostachowicz3Ewa Węgrzynowicz4Jan Lazur5Agnieszka Szewczyk6Bożena Muszyńska7Department of Medicinal Plant and Mushroom Biotechnology, Faculty of Pharmacy, Jagiellonian University Medical College, 9 Medyczna, 30-688 Kraków, PolandDepartment of Medicinal Plant and Mushroom Biotechnology, Faculty of Pharmacy, Jagiellonian University Medical College, 9 Medyczna, 30-688 Kraków, PolandDepartment of Medicinal Plant and Mushroom Biotechnology, Faculty of Pharmacy, Jagiellonian University Medical College, 9 Medyczna, 30-688 Kraków, PolandDepartment of Medical Physics and Biophysics, Faculty of Physics and Applied Computer Science, AGH University of Krakow, 30 Adama Mickiewicza, 30-059 Kraków, PolandDepartment of Medicinal Plant and Mushroom Biotechnology, Faculty of Pharmacy, Jagiellonian University Medical College, 9 Medyczna, 30-688 Kraków, PolandDepartment of Medicinal Plant and Mushroom Biotechnology, Faculty of Pharmacy, Jagiellonian University Medical College, 9 Medyczna, 30-688 Kraków, PolandDepartment of Medicinal Plant and Mushroom Biotechnology, Faculty of Pharmacy, Jagiellonian University Medical College, 9 Medyczna, 30-688 Kraków, PolandDepartment of Medicinal Plant and Mushroom Biotechnology, Faculty of Pharmacy, Jagiellonian University Medical College, 9 Medyczna, 30-688 Kraków, PolandThe selection of plant-based substrates for mushroom cultivation is a key factor influencing their growth and metabolism. The aim of this study was to demonstrate, in an innovative approach, differences in the content of biologically active compounds, bioelements, and antioxidant properties of <i>Hericium erinaceus</i> (Bull.) Pers. cultivated on various plant-based substrates derived from waste materials, specifically hemp straw and beech sawdust. Another objective was to compare various extraction methods in terms of their impact on the concentration of these compounds. Elemental analysis was performed using the TXRF method, while bioactive constituents were determined using the DAD/UV RP-HPLC technique. The plant-based substrate and extraction method influenced the levels of obtained metabolites. Dual extraction with moderate ethanol concentrations was most effective for isolating key bioactive compounds from <i>H. erinaceus</i>—notably ergothioneine, lovastatin, L-phenylalanine, and ergosterol—while antioxidant activity did not correlate with the concentration of the solvent used. Although dual extracts enhanced certain antioxidants and metabolites, whole fruiting bodies contained higher levels of bioelements. Overall, fruiting bodies grown on beech sawdust had greater amounts of most bioactive compounds compared to those cultivated on hemp straw, emphasizing that both substrate choice and extraction method critically influence the mushroom’s bioactive profile and its potential health benefits.https://www.mdpi.com/1420-3049/30/15/3094<i>Hericium erinaceus</i> (Bull.) Pers.plant-based cultivation substratesnatural productsantioxidant activitybioactive compoundsbioelements |
| spellingShingle | Katarzyna Kała Małgorzata Cicha-Jeleń Katarzyna Sułkowska-Ziaja Beata Ostachowicz Ewa Węgrzynowicz Jan Lazur Agnieszka Szewczyk Bożena Muszyńska Influence of Plant-Based Substrate Composition and Extraction Method on Accumulation of Bioactive Compounds in <i>Hericium erinaceus</i> (Bull.) Pers. Fruiting Bodies Molecules <i>Hericium erinaceus</i> (Bull.) Pers. plant-based cultivation substrates natural products antioxidant activity bioactive compounds bioelements |
| title | Influence of Plant-Based Substrate Composition and Extraction Method on Accumulation of Bioactive Compounds in <i>Hericium erinaceus</i> (Bull.) Pers. Fruiting Bodies |
| title_full | Influence of Plant-Based Substrate Composition and Extraction Method on Accumulation of Bioactive Compounds in <i>Hericium erinaceus</i> (Bull.) Pers. Fruiting Bodies |
| title_fullStr | Influence of Plant-Based Substrate Composition and Extraction Method on Accumulation of Bioactive Compounds in <i>Hericium erinaceus</i> (Bull.) Pers. Fruiting Bodies |
| title_full_unstemmed | Influence of Plant-Based Substrate Composition and Extraction Method on Accumulation of Bioactive Compounds in <i>Hericium erinaceus</i> (Bull.) Pers. Fruiting Bodies |
| title_short | Influence of Plant-Based Substrate Composition and Extraction Method on Accumulation of Bioactive Compounds in <i>Hericium erinaceus</i> (Bull.) Pers. Fruiting Bodies |
| title_sort | influence of plant based substrate composition and extraction method on accumulation of bioactive compounds in i hericium erinaceus i bull pers fruiting bodies |
| topic | <i>Hericium erinaceus</i> (Bull.) Pers. plant-based cultivation substrates natural products antioxidant activity bioactive compounds bioelements |
| url | https://www.mdpi.com/1420-3049/30/15/3094 |
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