Comparative Detection and Genetic Characterization of Feline Panleukopenia Virus in Bangladesh
ABSTRACT Background Feline panleukopenia virus (FPV) is a highly contagious and often fatal disease affecting domestic and wild felines. Accurate diagnosis and understanding of circulating strains are essential for effective control. Objectives This study aimed to evaluate the diagnostic accuracy of...
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Wiley
2025-09-01
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| Series: | Veterinary Medicine and Science |
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| Online Access: | https://doi.org/10.1002/vms3.70594 |
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| author | Nurejunnati Jeba Roni Mia Md. Masum Billah Tarafder Anandha Mozumder Raduyan Farazi S. M. Nazmul Hasan Mohammad Bayazid Bostami A. K. M. Anisur Rahman Abdul Mannan Sharmin Akter Sukumar Saha Tofazzal Islam Elcio Leal Antonio Charlys da Costa Md. Golzar Hossain |
| author_facet | Nurejunnati Jeba Roni Mia Md. Masum Billah Tarafder Anandha Mozumder Raduyan Farazi S. M. Nazmul Hasan Mohammad Bayazid Bostami A. K. M. Anisur Rahman Abdul Mannan Sharmin Akter Sukumar Saha Tofazzal Islam Elcio Leal Antonio Charlys da Costa Md. Golzar Hossain |
| author_sort | Nurejunnati Jeba |
| collection | DOAJ |
| description | ABSTRACT Background Feline panleukopenia virus (FPV) is a highly contagious and often fatal disease affecting domestic and wild felines. Accurate diagnosis and understanding of circulating strains are essential for effective control. Objectives This study aimed to evaluate the diagnostic accuracy of a rapid immunochromatographic (IC) antigen test compared to PCR for FPV detection in clinically suspected pet cats in Bangladesh. It also aimed to investigate the genetic and evolutionary characteristics of circulating FPV strains. Methods Faecal or rectal swab samples from suspected cats were tested using both IC strip tests and PCR. Sensitivity and specificity of the IC test were analysed using PCR as the reference. Partial sequencing of the VP2 gene was performed on four PCR‐positive samples for phylogenetic and mutational analysis. Structural modelling of VP2 proteins was conducted to predict conformational changes. Results The IC test detected FPV in 84% of cases, whereas PCR confirmed only 60%, indicating a 24% false‐positive rate. PCR showed higher diagnostic reliability. FPV prevalence was 92% among unvaccinated cats. Phylogenetic analysis of VP2 sequences revealed close genetic similarity with Chinese and Portuguese strains, suggesting possible cross‐border transmission. Mutations such as A756G, A896G, E299G and T236I were consistently observed. Structural modelling indicated minor conformational changes in VP2. Conclusion and clinical significance PCR offers superior accuracy over IC testing for FPV diagnosis. Mutational changes may impact antigenicity and diagnostic performance. Improved diagnostic accuracy, molecular surveillance and updated vaccination strategies are essential to control FPV outbreaks in feline populations. |
| format | Article |
| id | doaj-art-d363fb71fe95457d97231078eacdf1eb |
| institution | Kabale University |
| issn | 2053-1095 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine and Science |
| spelling | doaj-art-d363fb71fe95457d97231078eacdf1eb2025-08-22T15:05:05ZengWileyVeterinary Medicine and Science2053-10952025-09-01115n/an/a10.1002/vms3.70594Comparative Detection and Genetic Characterization of Feline Panleukopenia Virus in BangladeshNurejunnati Jeba0Roni Mia1Md. Masum Billah Tarafder2Anandha Mozumder3Raduyan Farazi4S. M. Nazmul Hasan5Mohammad Bayazid Bostami6A. K. M. Anisur Rahman7Abdul Mannan8Sharmin Akter9Sukumar Saha10Tofazzal Islam11Elcio Leal12Antonio Charlys da Costa13Md. Golzar Hossain14Department of Microbiology and Hygiene Bangladesh Agricultural University Mymensingh BangladeshDepartment of Microbiology and Hygiene Bangladesh Agricultural University Mymensingh BangladeshDepartment of Microbiology and Hygiene Bangladesh Agricultural University Mymensingh BangladeshDepartment of Microbiology and Hygiene Bangladesh Agricultural University Mymensingh BangladeshDepartment of Microbiology and Hygiene Bangladesh Agricultural University Mymensingh BangladeshDepartment of Microbiology and Hygiene Bangladesh Agricultural University Mymensingh BangladeshTeaching and Training Pet Hospital and Research Center Chattogram Veterinary and Animal Sciences University Chattogram BangladeshDepartment of Medicine Bangladesh Agricultural University Mymensingh BangladeshTeaching and Training Pet Hospital and Research Center Chattogram Veterinary and Animal Sciences University Chattogram BangladeshDepartment of Physiology Bangladesh Agricultural University Mymensingh BangladeshDepartment of Microbiology and Hygiene Bangladesh Agricultural University Mymensingh BangladeshInstitute of Biotechnology and Genetic Engineering Gazipur Agricultural University Gazipur BangladeshInstitute of Biological Sciences Federal University of Pará Belem Pará BrazilInstituto de Medicina Tropical Faculdade de Medicina Universidade de São Paulo São Paulo, State of São Paulo BrazilDepartment of Microbiology and Hygiene Bangladesh Agricultural University Mymensingh BangladeshABSTRACT Background Feline panleukopenia virus (FPV) is a highly contagious and often fatal disease affecting domestic and wild felines. Accurate diagnosis and understanding of circulating strains are essential for effective control. Objectives This study aimed to evaluate the diagnostic accuracy of a rapid immunochromatographic (IC) antigen test compared to PCR for FPV detection in clinically suspected pet cats in Bangladesh. It also aimed to investigate the genetic and evolutionary characteristics of circulating FPV strains. Methods Faecal or rectal swab samples from suspected cats were tested using both IC strip tests and PCR. Sensitivity and specificity of the IC test were analysed using PCR as the reference. Partial sequencing of the VP2 gene was performed on four PCR‐positive samples for phylogenetic and mutational analysis. Structural modelling of VP2 proteins was conducted to predict conformational changes. Results The IC test detected FPV in 84% of cases, whereas PCR confirmed only 60%, indicating a 24% false‐positive rate. PCR showed higher diagnostic reliability. FPV prevalence was 92% among unvaccinated cats. Phylogenetic analysis of VP2 sequences revealed close genetic similarity with Chinese and Portuguese strains, suggesting possible cross‐border transmission. Mutations such as A756G, A896G, E299G and T236I were consistently observed. Structural modelling indicated minor conformational changes in VP2. Conclusion and clinical significance PCR offers superior accuracy over IC testing for FPV diagnosis. Mutational changes may impact antigenicity and diagnostic performance. Improved diagnostic accuracy, molecular surveillance and updated vaccination strategies are essential to control FPV outbreaks in feline populations.https://doi.org/10.1002/vms3.70594Feline panleukopenia virusIC strip testPCRphylogenetic analysisVP2 gene mutations |
| spellingShingle | Nurejunnati Jeba Roni Mia Md. Masum Billah Tarafder Anandha Mozumder Raduyan Farazi S. M. Nazmul Hasan Mohammad Bayazid Bostami A. K. M. Anisur Rahman Abdul Mannan Sharmin Akter Sukumar Saha Tofazzal Islam Elcio Leal Antonio Charlys da Costa Md. Golzar Hossain Comparative Detection and Genetic Characterization of Feline Panleukopenia Virus in Bangladesh Veterinary Medicine and Science Feline panleukopenia virus IC strip test PCR phylogenetic analysis VP2 gene mutations |
| title | Comparative Detection and Genetic Characterization of Feline Panleukopenia Virus in Bangladesh |
| title_full | Comparative Detection and Genetic Characterization of Feline Panleukopenia Virus in Bangladesh |
| title_fullStr | Comparative Detection and Genetic Characterization of Feline Panleukopenia Virus in Bangladesh |
| title_full_unstemmed | Comparative Detection and Genetic Characterization of Feline Panleukopenia Virus in Bangladesh |
| title_short | Comparative Detection and Genetic Characterization of Feline Panleukopenia Virus in Bangladesh |
| title_sort | comparative detection and genetic characterization of feline panleukopenia virus in bangladesh |
| topic | Feline panleukopenia virus IC strip test PCR phylogenetic analysis VP2 gene mutations |
| url | https://doi.org/10.1002/vms3.70594 |
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