Characterisation of the arsenic resistance genes in Bacillus sp. UWC isolated from maturing fly ash acid mine drainage neutralised solids
An arsenic resistant Bacillus sp. UWC was isolated from fly ash acid mine drainage (FA-AMD) neutralised solids. A genomic library was prepared and screened in an arsenic sensitive mutant Escherichia coli strain for the presence of arsenic resistance (ars) genes. Sequence analysis of a clone conferri...
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| Format: | Article |
| Language: | English |
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Academy of Science of South Africa
2010-03-01
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| Series: | South African Journal of Science |
| Online Access: | https://sajs.co.za/article/view/10190 |
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| author | Wicleffe Musingarimi Marla Tuffin Donald Cowan |
| author_facet | Wicleffe Musingarimi Marla Tuffin Donald Cowan |
| author_sort | Wicleffe Musingarimi |
| collection | DOAJ |
| description | An arsenic resistant Bacillus sp. UWC was isolated from fly ash acid mine drainage (FA-AMD) neutralised solids. A genomic library was prepared and screened in an arsenic sensitive mutant Escherichia coli strain for the presence of arsenic resistance (ars) genes. Sequence analysis of a clone conferring resistance to both sodium arsenite and sodium arsenate revealed homologues to the arsR (regulatory repressor), arsB (membrane located arsenite pump), arsC (arsenate reductase), arsD (second regulatory repressor and a metallochaperone) and arsA (ATPase) genes from known arsenic resistance operons. The Bacillus sp. UWC arsRBCDA genes were shown to be arranged in an unusual manner with the arsDA genes immediately downstream of arsC. |
| format | Article |
| id | doaj-art-d35be72706184883b65bbfc2ffc31eb8 |
| institution | OA Journals |
| issn | 1996-7489 |
| language | English |
| publishDate | 2010-03-01 |
| publisher | Academy of Science of South Africa |
| record_format | Article |
| series | South African Journal of Science |
| spelling | doaj-art-d35be72706184883b65bbfc2ffc31eb82025-08-20T02:25:59ZengAcademy of Science of South AfricaSouth African Journal of Science1996-74892010-03-011061/259638392Characterisation of the arsenic resistance genes in Bacillus sp. UWC isolated from maturing fly ash acid mine drainage neutralised solidsWicleffe Musingarimi0Marla Tuffin1Donald Cowan2University of the Western CapeUniversity of the Western CapeUniversity of the Western CapeAn arsenic resistant Bacillus sp. UWC was isolated from fly ash acid mine drainage (FA-AMD) neutralised solids. A genomic library was prepared and screened in an arsenic sensitive mutant Escherichia coli strain for the presence of arsenic resistance (ars) genes. Sequence analysis of a clone conferring resistance to both sodium arsenite and sodium arsenate revealed homologues to the arsR (regulatory repressor), arsB (membrane located arsenite pump), arsC (arsenate reductase), arsD (second regulatory repressor and a metallochaperone) and arsA (ATPase) genes from known arsenic resistance operons. The Bacillus sp. UWC arsRBCDA genes were shown to be arranged in an unusual manner with the arsDA genes immediately downstream of arsC.https://sajs.co.za/article/view/10190 |
| spellingShingle | Wicleffe Musingarimi Marla Tuffin Donald Cowan Characterisation of the arsenic resistance genes in Bacillus sp. UWC isolated from maturing fly ash acid mine drainage neutralised solids South African Journal of Science |
| title | Characterisation of the arsenic resistance genes in Bacillus sp. UWC isolated from maturing fly ash acid mine drainage neutralised solids |
| title_full | Characterisation of the arsenic resistance genes in Bacillus sp. UWC isolated from maturing fly ash acid mine drainage neutralised solids |
| title_fullStr | Characterisation of the arsenic resistance genes in Bacillus sp. UWC isolated from maturing fly ash acid mine drainage neutralised solids |
| title_full_unstemmed | Characterisation of the arsenic resistance genes in Bacillus sp. UWC isolated from maturing fly ash acid mine drainage neutralised solids |
| title_short | Characterisation of the arsenic resistance genes in Bacillus sp. UWC isolated from maturing fly ash acid mine drainage neutralised solids |
| title_sort | characterisation of the arsenic resistance genes in bacillus sp uwc isolated from maturing fly ash acid mine drainage neutralised solids |
| url | https://sajs.co.za/article/view/10190 |
| work_keys_str_mv | AT wicleffemusingarimi characterisationofthearsenicresistancegenesinbacillusspuwcisolatedfrommaturingflyashacidminedrainageneutralisedsolids AT marlatuffin characterisationofthearsenicresistancegenesinbacillusspuwcisolatedfrommaturingflyashacidminedrainageneutralisedsolids AT donaldcowan characterisationofthearsenicresistancegenesinbacillusspuwcisolatedfrommaturingflyashacidminedrainageneutralisedsolids |