Prenatal Rosiglitazone Administration to Neonatal Rat Pups Does Not Alter the Adult Metabolic Phenotype
Prenatally administered rosiglitazone (RGZ) is effective in enhancing lung maturity; however, its long-term safety remains unknown. This study aimed to determine the effects of prenatally administered RGZ on the metabolic phenotype of adult rats. Methods. Pregnant Sprague-Dawley rat dams were admini...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2012-01-01
|
| Series: | PPAR Research |
| Online Access: | http://dx.doi.org/10.1155/2012/604216 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849473210724122624 |
|---|---|
| author | Hernan Sierra Reiko Sakurai W. N. Paul Lee Nghia C. Truong John S. Torday Virender K. Rehan |
| author_facet | Hernan Sierra Reiko Sakurai W. N. Paul Lee Nghia C. Truong John S. Torday Virender K. Rehan |
| author_sort | Hernan Sierra |
| collection | DOAJ |
| description | Prenatally administered rosiglitazone (RGZ) is effective in enhancing lung maturity; however, its long-term safety remains unknown. This study aimed to determine the effects of prenatally administered RGZ on the metabolic phenotype of adult rats. Methods. Pregnant Sprague-Dawley rat dams were administered either placebo or RGZ at embryonic days 18 and 19. Between 12 and 20 weeks of age, the rats underwent glucose and insulin tolerance tests and de novo fatty acid synthesis assays. The lungs, liver, skeletal muscle, and fat tissue were processed by Western hybridization for peroxisome proliferator-activated receptor (PPAR)γ, adipose differentiation-related protein (ADRP), and surfactant proteins B (SPB) and C (SPC). Plasma was assayed for triglycerides, cholesterol, insulin, glucagon, and troponin-I levels. Lungs were also morphometrically analyzed. Results. Insulin and glucose challenges, de novo fatty acid synthesis, and all serum assays revealed no differences among all groups. Western hybridization for PPARγ, ADRP, SPB, and SPC in lung, liver, muscle, and fat tissue showed equal levels. Histologic analyses showed a similar number of alveoli and septal thickness in all experimental groups. Conclusions. When administered prenatally, RGZ does not affect long-term fetal programming and may be safe for enhancing fetal lung maturation. |
| format | Article |
| id | doaj-art-d3574455a242400e862bfd4b20e47397 |
| institution | Kabale University |
| issn | 1687-4757 1687-4765 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | PPAR Research |
| spelling | doaj-art-d3574455a242400e862bfd4b20e473972025-08-20T03:24:13ZengWileyPPAR Research1687-47571687-47652012-01-01201210.1155/2012/604216604216Prenatal Rosiglitazone Administration to Neonatal Rat Pups Does Not Alter the Adult Metabolic PhenotypeHernan Sierra0Reiko Sakurai1W. N. Paul Lee2Nghia C. Truong3John S. Torday4Virender K. Rehan5Department of Pediatrics, Los Angeles Biomedical Research Institute, Harbor-UCLA Medical Center and David Geffen School of Medicine, University of California, Los Angeles, Torrance, CA 90502, USADepartment of Pediatrics, Los Angeles Biomedical Research Institute, Harbor-UCLA Medical Center and David Geffen School of Medicine, University of California, Los Angeles, Torrance, CA 90502, USADepartment of Pediatrics, Los Angeles Biomedical Research Institute, Harbor-UCLA Medical Center and David Geffen School of Medicine, University of California, Los Angeles, Torrance, CA 90502, USADepartment of Pediatrics, Los Angeles Biomedical Research Institute, Harbor-UCLA Medical Center and David Geffen School of Medicine, University of California, Los Angeles, Torrance, CA 90502, USADepartment of Pediatrics, Los Angeles Biomedical Research Institute, Harbor-UCLA Medical Center and David Geffen School of Medicine, University of California, Los Angeles, Torrance, CA 90502, USADepartment of Pediatrics, Los Angeles Biomedical Research Institute, Harbor-UCLA Medical Center and David Geffen School of Medicine, University of California, Los Angeles, Torrance, CA 90502, USAPrenatally administered rosiglitazone (RGZ) is effective in enhancing lung maturity; however, its long-term safety remains unknown. This study aimed to determine the effects of prenatally administered RGZ on the metabolic phenotype of adult rats. Methods. Pregnant Sprague-Dawley rat dams were administered either placebo or RGZ at embryonic days 18 and 19. Between 12 and 20 weeks of age, the rats underwent glucose and insulin tolerance tests and de novo fatty acid synthesis assays. The lungs, liver, skeletal muscle, and fat tissue were processed by Western hybridization for peroxisome proliferator-activated receptor (PPAR)γ, adipose differentiation-related protein (ADRP), and surfactant proteins B (SPB) and C (SPC). Plasma was assayed for triglycerides, cholesterol, insulin, glucagon, and troponin-I levels. Lungs were also morphometrically analyzed. Results. Insulin and glucose challenges, de novo fatty acid synthesis, and all serum assays revealed no differences among all groups. Western hybridization for PPARγ, ADRP, SPB, and SPC in lung, liver, muscle, and fat tissue showed equal levels. Histologic analyses showed a similar number of alveoli and septal thickness in all experimental groups. Conclusions. When administered prenatally, RGZ does not affect long-term fetal programming and may be safe for enhancing fetal lung maturation.http://dx.doi.org/10.1155/2012/604216 |
| spellingShingle | Hernan Sierra Reiko Sakurai W. N. Paul Lee Nghia C. Truong John S. Torday Virender K. Rehan Prenatal Rosiglitazone Administration to Neonatal Rat Pups Does Not Alter the Adult Metabolic Phenotype PPAR Research |
| title | Prenatal Rosiglitazone Administration to Neonatal Rat Pups Does Not Alter the Adult Metabolic Phenotype |
| title_full | Prenatal Rosiglitazone Administration to Neonatal Rat Pups Does Not Alter the Adult Metabolic Phenotype |
| title_fullStr | Prenatal Rosiglitazone Administration to Neonatal Rat Pups Does Not Alter the Adult Metabolic Phenotype |
| title_full_unstemmed | Prenatal Rosiglitazone Administration to Neonatal Rat Pups Does Not Alter the Adult Metabolic Phenotype |
| title_short | Prenatal Rosiglitazone Administration to Neonatal Rat Pups Does Not Alter the Adult Metabolic Phenotype |
| title_sort | prenatal rosiglitazone administration to neonatal rat pups does not alter the adult metabolic phenotype |
| url | http://dx.doi.org/10.1155/2012/604216 |
| work_keys_str_mv | AT hernansierra prenatalrosiglitazoneadministrationtoneonatalratpupsdoesnotaltertheadultmetabolicphenotype AT reikosakurai prenatalrosiglitazoneadministrationtoneonatalratpupsdoesnotaltertheadultmetabolicphenotype AT wnpaullee prenatalrosiglitazoneadministrationtoneonatalratpupsdoesnotaltertheadultmetabolicphenotype AT nghiactruong prenatalrosiglitazoneadministrationtoneonatalratpupsdoesnotaltertheadultmetabolicphenotype AT johnstorday prenatalrosiglitazoneadministrationtoneonatalratpupsdoesnotaltertheadultmetabolicphenotype AT virenderkrehan prenatalrosiglitazoneadministrationtoneonatalratpupsdoesnotaltertheadultmetabolicphenotype |