Characteristics and influencing factors of gut microbiota in population with sleep disorders
IntroductionThe integrated analysis of gut and oral microbiota and their metabolites helps elucidate key factors affecting sleep disorders in populations and provides research insights for understanding sleep regulation mechanisms.MethodsBased on a cross-sectional study design, this research combine...
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Frontiers Media S.A.
2025-07-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1586864/full |
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| author | Chunhong Zhang Chunhong Zhang Chunhong Zhang Qinglin Sheng Yuanqi Wang Qi Shen Yifei Zhai Dawei Hu Nanjia Zhang Ziyuan Wang Xuebin Yin Dan Li Youtao Chen |
| author_facet | Chunhong Zhang Chunhong Zhang Chunhong Zhang Qinglin Sheng Yuanqi Wang Qi Shen Yifei Zhai Dawei Hu Nanjia Zhang Ziyuan Wang Xuebin Yin Dan Li Youtao Chen |
| author_sort | Chunhong Zhang |
| collection | DOAJ |
| description | IntroductionThe integrated analysis of gut and oral microbiota and their metabolites helps elucidate key factors affecting sleep disorders in populations and provides research insights for understanding sleep regulation mechanisms.MethodsBased on a cross-sectional study design, this research combined 16S sequencing and untargeted metabolomics to investigate lifestyle habits and physical conditions of 165 adult male subjects, systematically examining characteristics of gut and oral microbiota and their metabolites.ResultsAnalysis of gut microbiota revealed significantly reduced microbial diversity in the insomnia group, with predominant phyla being Firmicutes, Actinobacteriota, and Bacteroidetes. At the genus level, the abundance of Blautia was significantly elevated. Gut metabolite analysis showed significant enrichment in metabolic pathways such as “phenylalanine, tyrosine, and tryptophan biosynthesis.” Regarding oral microbiota, no significant difference in diversity was observed between sleepless and normal groups. At the genus level, the sleepless group showed significantly decreased abundance of Streptococcus and increased abundance of Veillonella. Metabolite analysis indicated significant correlation between the sleepless group and metabolic pathways such as “pantothenate and CoA biosynthesis.”DiscussionThis study compared differences in gut and oral microbiota and metabolites between sleepless and normal groups, identifying potential biomarkers for insomnia, including gut Blautia, aromatic amino acid metabolites, salivary Streptococcus and Veillonella, and pantothenate-related metabolites. These findings provide important multi-omics data for investigating the pathological mechanisms of insomnia. We have made changes according to the requirements, please adjust according to the standard. |
| format | Article |
| id | doaj-art-d356754fd1be4a8aae78386d7ddb68ce |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-d356754fd1be4a8aae78386d7ddb68ce2025-08-20T03:50:27ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-07-011610.3389/fmicb.2025.15868641586864Characteristics and influencing factors of gut microbiota in population with sleep disordersChunhong Zhang0Chunhong Zhang1Chunhong Zhang2Qinglin Sheng3Yuanqi Wang4Qi Shen5Yifei Zhai6Dawei Hu7Nanjia Zhang8Ziyuan Wang9Xuebin Yin10Dan Li11Youtao Chen12Institute of Food Science and Technology, CAAS, Beijing, ChinaGuangdong Provincial Key Laboratory of Intelligent Port Security Inspection, Guangzhou, ChinaAnhui Province Key Laboratory of Functional Agriculture and Functional Food, Anhui Science and Technology University, Chuzhou, ChinaSchool of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai, ChinaAnhui Province Key Laboratory of Functional Agriculture and Functional Food, Anhui Science and Technology University, Chuzhou, ChinaSchool of Basic Medical Science, Naval Medical University, Shanghai, ChinaCollege of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, ChinaGuangdong Provincial Key Laboratory of Intelligent Port Security Inspection, Guangzhou, ChinaSchool of Basic Medical Science, Naval Medical University, Shanghai, ChinaSchool of Basic Medical Science, Naval Medical University, Shanghai, ChinaAnhui Province Key Laboratory of Functional Agriculture and Functional Food, Anhui Science and Technology University, Chuzhou, ChinaPLA Navy Medical Center, Shanghai, ChinaAnhui Province Key Laboratory of Functional Agriculture and Functional Food, Anhui Science and Technology University, Chuzhou, ChinaIntroductionThe integrated analysis of gut and oral microbiota and their metabolites helps elucidate key factors affecting sleep disorders in populations and provides research insights for understanding sleep regulation mechanisms.MethodsBased on a cross-sectional study design, this research combined 16S sequencing and untargeted metabolomics to investigate lifestyle habits and physical conditions of 165 adult male subjects, systematically examining characteristics of gut and oral microbiota and their metabolites.ResultsAnalysis of gut microbiota revealed significantly reduced microbial diversity in the insomnia group, with predominant phyla being Firmicutes, Actinobacteriota, and Bacteroidetes. At the genus level, the abundance of Blautia was significantly elevated. Gut metabolite analysis showed significant enrichment in metabolic pathways such as “phenylalanine, tyrosine, and tryptophan biosynthesis.” Regarding oral microbiota, no significant difference in diversity was observed between sleepless and normal groups. At the genus level, the sleepless group showed significantly decreased abundance of Streptococcus and increased abundance of Veillonella. Metabolite analysis indicated significant correlation between the sleepless group and metabolic pathways such as “pantothenate and CoA biosynthesis.”DiscussionThis study compared differences in gut and oral microbiota and metabolites between sleepless and normal groups, identifying potential biomarkers for insomnia, including gut Blautia, aromatic amino acid metabolites, salivary Streptococcus and Veillonella, and pantothenate-related metabolites. These findings provide important multi-omics data for investigating the pathological mechanisms of insomnia. We have made changes according to the requirements, please adjust according to the standard.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1586864/fullinsomniagutoral cavitymicrobial florametabolomics |
| spellingShingle | Chunhong Zhang Chunhong Zhang Chunhong Zhang Qinglin Sheng Yuanqi Wang Qi Shen Yifei Zhai Dawei Hu Nanjia Zhang Ziyuan Wang Xuebin Yin Dan Li Youtao Chen Characteristics and influencing factors of gut microbiota in population with sleep disorders Frontiers in Microbiology insomnia gut oral cavity microbial flora metabolomics |
| title | Characteristics and influencing factors of gut microbiota in population with sleep disorders |
| title_full | Characteristics and influencing factors of gut microbiota in population with sleep disorders |
| title_fullStr | Characteristics and influencing factors of gut microbiota in population with sleep disorders |
| title_full_unstemmed | Characteristics and influencing factors of gut microbiota in population with sleep disorders |
| title_short | Characteristics and influencing factors of gut microbiota in population with sleep disorders |
| title_sort | characteristics and influencing factors of gut microbiota in population with sleep disorders |
| topic | insomnia gut oral cavity microbial flora metabolomics |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1586864/full |
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