Evaluation of utilization of amplified blastocoel fluid DNA gel electrophoresis band intensity as an additional minimally invasive approach in embryo selection: A cross-sectional study
Background: Embryo selection for transfer is critical in assisted reproduction. The presence of DNA in the blastocoel cavity of human blastocysts is assumed to be a consequence of common preimplantation chromosomal abnormalities. Objective: This study examined the relationship between the amount...
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Shahid Sadoughi University of Medical Sciences
2025-01-01
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| Series: | International Journal of Reproductive BioMedicine |
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| Online Access: | https://knepublishing.com/index.php/ijrm/article/view/17823 |
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| author | Fattaneh Khajehoseini Zahra Noormohammadi Poopak Eftekhari-Yazdi Hamid Gourabi Reza Pazhoomand Shirzad Hosseinishenatal Masood Bazrgar |
| author_facet | Fattaneh Khajehoseini Zahra Noormohammadi Poopak Eftekhari-Yazdi Hamid Gourabi Reza Pazhoomand Shirzad Hosseinishenatal Masood Bazrgar |
| author_sort | Fattaneh Khajehoseini |
| collection | DOAJ |
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Background: Embryo selection for transfer is critical in assisted reproduction. The presence of DNA in the blastocoel cavity of human blastocysts is assumed to be a consequence of common preimplantation chromosomal abnormalities.
Objective: This study examined the relationship between the amount of blastocoel fluid (BF) DNA and the band intensity of amplified BF-DNA in gel electrophoresis, considering the influence of ploidy status.
Materials and Methods: This cross-sectional study categorizes blastocysts into 2 groups based on the array comparative genomic hybridization results by trophectoderm biopsy -the euploid and aneuploid group. After morphological scoring, a biopsy of BF was performed for whole genome amplification, followed by an assessment of band intensity and BF-DNA quantification. The relationship between BF-DNA levels, band intensity, and ploidy status were analyzed.
Results: The level of BF-DNA was higher in the aneuploid group compared to the euploid group, but the difference was not statistically significant (p = 0.2). We observed that the band intensity was affected by the ploidy status of the embryos, although this difference was not statistically significant (p = 0.059). Notably, electrophoresis band of all embryos with chromosomal loss was strong. No correlation was observed between embryo morphology and chromosomal ploidy (p = 0.8).
Conclusion: Our findings indicate that BF-DNA band intensity on agarose gel is not currently applicable for preimplantation embryo selection. It seems that embryos with chromosomal loss are more prone to DNA release to BF. The disrelation between embryo morphology and ploidy status represents the necessity of minimally invasive embryo screening methods based on ploidy status.
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| format | Article |
| id | doaj-art-d3519037b06a4ea7986afc86d55a119d |
| institution | DOAJ |
| issn | 2476-4108 2476-3772 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Shahid Sadoughi University of Medical Sciences |
| record_format | Article |
| series | International Journal of Reproductive BioMedicine |
| spelling | doaj-art-d3519037b06a4ea7986afc86d55a119d2025-08-20T03:01:35ZengShahid Sadoughi University of Medical SciencesInternational Journal of Reproductive BioMedicine2476-41082476-37722025-01-01221110.18502/ijrm.v22i11.17823Evaluation of utilization of amplified blastocoel fluid DNA gel electrophoresis band intensity as an additional minimally invasive approach in embryo selection: A cross-sectional studyFattaneh Khajehoseini0Zahra Noormohammadi1Poopak Eftekhari-Yazdi2Hamid Gourabi3Reza Pazhoomand4Shirzad Hosseinishenatal5Masood Bazrgar6Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.Medical Genetics Laboratory, Shiraz Fertility Center, Shiraz, Iran.Department of Embryology, Shiraz Fertility Center, Shiraz, Iran.Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran. Background: Embryo selection for transfer is critical in assisted reproduction. The presence of DNA in the blastocoel cavity of human blastocysts is assumed to be a consequence of common preimplantation chromosomal abnormalities. Objective: This study examined the relationship between the amount of blastocoel fluid (BF) DNA and the band intensity of amplified BF-DNA in gel electrophoresis, considering the influence of ploidy status. Materials and Methods: This cross-sectional study categorizes blastocysts into 2 groups based on the array comparative genomic hybridization results by trophectoderm biopsy -the euploid and aneuploid group. After morphological scoring, a biopsy of BF was performed for whole genome amplification, followed by an assessment of band intensity and BF-DNA quantification. The relationship between BF-DNA levels, band intensity, and ploidy status were analyzed. Results: The level of BF-DNA was higher in the aneuploid group compared to the euploid group, but the difference was not statistically significant (p = 0.2). We observed that the band intensity was affected by the ploidy status of the embryos, although this difference was not statistically significant (p = 0.059). Notably, electrophoresis band of all embryos with chromosomal loss was strong. No correlation was observed between embryo morphology and chromosomal ploidy (p = 0.8). Conclusion: Our findings indicate that BF-DNA band intensity on agarose gel is not currently applicable for preimplantation embryo selection. It seems that embryos with chromosomal loss are more prone to DNA release to BF. The disrelation between embryo morphology and ploidy status represents the necessity of minimally invasive embryo screening methods based on ploidy status. https://knepublishing.com/index.php/ijrm/article/view/17823Aneuploidy, Blastocyst, Blastocoel fluid, Human embryo, Preimplantation genetic testing. |
| spellingShingle | Fattaneh Khajehoseini Zahra Noormohammadi Poopak Eftekhari-Yazdi Hamid Gourabi Reza Pazhoomand Shirzad Hosseinishenatal Masood Bazrgar Evaluation of utilization of amplified blastocoel fluid DNA gel electrophoresis band intensity as an additional minimally invasive approach in embryo selection: A cross-sectional study International Journal of Reproductive BioMedicine Aneuploidy, Blastocyst, Blastocoel fluid, Human embryo, Preimplantation genetic testing. |
| title | Evaluation of utilization of amplified blastocoel fluid DNA gel electrophoresis band intensity as an additional minimally invasive approach in embryo selection: A cross-sectional study |
| title_full | Evaluation of utilization of amplified blastocoel fluid DNA gel electrophoresis band intensity as an additional minimally invasive approach in embryo selection: A cross-sectional study |
| title_fullStr | Evaluation of utilization of amplified blastocoel fluid DNA gel electrophoresis band intensity as an additional minimally invasive approach in embryo selection: A cross-sectional study |
| title_full_unstemmed | Evaluation of utilization of amplified blastocoel fluid DNA gel electrophoresis band intensity as an additional minimally invasive approach in embryo selection: A cross-sectional study |
| title_short | Evaluation of utilization of amplified blastocoel fluid DNA gel electrophoresis band intensity as an additional minimally invasive approach in embryo selection: A cross-sectional study |
| title_sort | evaluation of utilization of amplified blastocoel fluid dna gel electrophoresis band intensity as an additional minimally invasive approach in embryo selection a cross sectional study |
| topic | Aneuploidy, Blastocyst, Blastocoel fluid, Human embryo, Preimplantation genetic testing. |
| url | https://knepublishing.com/index.php/ijrm/article/view/17823 |
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