Fast and cost-effective production of transgenic potato microtubersof Gala and Milena varieties
Potato microtubers play an important role in the technology of seed potato production, since they have great advantages in storage, transportation and mechanization due to their small size and weight. For research purposes, it is also often necessary to obtain microtubers, in particular when creati...
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| Format: | Article |
| Language: | English |
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Julius Kühn-Institut
2025-08-01
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| Series: | Journal of Applied Botany and Food Quality |
| Online Access: | https://ojs.openagrar.de/index.php/JABFQ/article/view/17924 |
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| _version_ | 1849769861442437120 |
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| author | Anna Nizkorodova Alina Dmitrieva Maria Suvorova Bulat Iskakov |
| author_facet | Anna Nizkorodova Alina Dmitrieva Maria Suvorova Bulat Iskakov |
| author_sort | Anna Nizkorodova |
| collection | DOAJ |
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Potato microtubers play an important role in the technology of seed potato production, since they have great advantages in storage, transportation and mechanization due to their small size and weight. For research purposes, it is also often necessary to obtain microtubers, in particular when creating lines of transgenic potato plants. Unlike agricultural needs, research requires a small number of microtubers, but as fast as possible and with minimal financial costs. We propose a method for obtaining microtubers, which saves time for transferring in vitro studies to soil in 4-7 weeks compared to so called “high sucrose concentration” methods (high concentration of sucrose; reduced photoperiod). We designated the method as “2.5S” due to sucrose content (2.5%) in Murashige and Skoog medium which amount for each plant was reduced by approximately 3 times. That leads to a lack of nutrients and the rapid formation of microtubers. The proposed method was tested on two potato varieties and their transgenic lines; in case of one of the varieties, a reliable increase in the mass of microtubers by 1.6-3.6 times was recorded compared to the standard method of obtaining microtubers. The method is also cost-effective and does not require any additional labor costs from researchers.
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| format | Article |
| id | doaj-art-d34b4b3c6f6e43cca0b180dbc4bdd951 |
| institution | DOAJ |
| issn | 1613-9216 1439-040X |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Julius Kühn-Institut |
| record_format | Article |
| series | Journal of Applied Botany and Food Quality |
| spelling | doaj-art-d34b4b3c6f6e43cca0b180dbc4bdd9512025-08-20T03:03:16ZengJulius Kühn-InstitutJournal of Applied Botany and Food Quality1613-92161439-040X2025-08-019810.5073/JABFQ.2025.098.010Fast and cost-effective production of transgenic potato microtubersof Gala and Milena varietiesAnna Nizkorodova0https://orcid.org/0000-0002-1597-7207Alina Dmitrieva 1https://orcid.org/0009-0000-6913-7775Maria Suvorova2https://orcid.org/0000-0001-6505-5561Bulat Iskakov 3M. Aitkhozhin Institute of Molecular Biology and Biochemistry, Almaty, KazakhstanM. Aitkhozhin Institute of Molecular Biology and Biochemistry, Almaty, KazakhstanGenetics department, Biological Faculty, Al-Faraby State National University, Almaty, KazakhstanM. Aitkhozhin Institute of Molecular Biology and Biochemistry, Almaty, Kazakhstan Potato microtubers play an important role in the technology of seed potato production, since they have great advantages in storage, transportation and mechanization due to their small size and weight. For research purposes, it is also often necessary to obtain microtubers, in particular when creating lines of transgenic potato plants. Unlike agricultural needs, research requires a small number of microtubers, but as fast as possible and with minimal financial costs. We propose a method for obtaining microtubers, which saves time for transferring in vitro studies to soil in 4-7 weeks compared to so called “high sucrose concentration” methods (high concentration of sucrose; reduced photoperiod). We designated the method as “2.5S” due to sucrose content (2.5%) in Murashige and Skoog medium which amount for each plant was reduced by approximately 3 times. That leads to a lack of nutrients and the rapid formation of microtubers. The proposed method was tested on two potato varieties and their transgenic lines; in case of one of the varieties, a reliable increase in the mass of microtubers by 1.6-3.6 times was recorded compared to the standard method of obtaining microtubers. The method is also cost-effective and does not require any additional labor costs from researchers. https://ojs.openagrar.de/index.php/JABFQ/article/view/17924 |
| spellingShingle | Anna Nizkorodova Alina Dmitrieva Maria Suvorova Bulat Iskakov Fast and cost-effective production of transgenic potato microtubersof Gala and Milena varieties Journal of Applied Botany and Food Quality |
| title | Fast and cost-effective production of transgenic potato microtubersof Gala and Milena varieties |
| title_full | Fast and cost-effective production of transgenic potato microtubersof Gala and Milena varieties |
| title_fullStr | Fast and cost-effective production of transgenic potato microtubersof Gala and Milena varieties |
| title_full_unstemmed | Fast and cost-effective production of transgenic potato microtubersof Gala and Milena varieties |
| title_short | Fast and cost-effective production of transgenic potato microtubersof Gala and Milena varieties |
| title_sort | fast and cost effective production of transgenic potato microtubersof gala and milena varieties |
| url | https://ojs.openagrar.de/index.php/JABFQ/article/view/17924 |
| work_keys_str_mv | AT annanizkorodova fastandcosteffectiveproductionoftransgenicpotatomicrotubersofgalaandmilenavarieties AT alinadmitrieva fastandcosteffectiveproductionoftransgenicpotatomicrotubersofgalaandmilenavarieties AT mariasuvorova fastandcosteffectiveproductionoftransgenicpotatomicrotubersofgalaandmilenavarieties AT bulatiskakov fastandcosteffectiveproductionoftransgenicpotatomicrotubersofgalaandmilenavarieties |