Acidomelania saccharicola sp. nov., a new species of dark septate endophytes in Helotiales, with potential of controlling Fusarium wilt of banana

Banana wilt-like disease, triggered by Fusarium oxysporum, poses a severe threat to banana cultivation as a persistent soilborne pathogen. A strategy to effectively control this disease has yet to be found. Dark septate endophytes (DSEs) fungi have emerged as promising biocontrol agents, not only of...

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Main Authors: Qian Nong, Yan Zhang, Yanyan Long, Yanlu Chen, Liping Qin, Shanyu Lin, Fenghua Zeng, Ling Xie
Format: Article
Language:English
Published: Elsevier 2025-07-01
Series:Biological Control
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Online Access:http://www.sciencedirect.com/science/article/pii/S104996442500091X
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author Qian Nong
Yan Zhang
Yanyan Long
Yanlu Chen
Liping Qin
Shanyu Lin
Fenghua Zeng
Ling Xie
author_facet Qian Nong
Yan Zhang
Yanyan Long
Yanlu Chen
Liping Qin
Shanyu Lin
Fenghua Zeng
Ling Xie
author_sort Qian Nong
collection DOAJ
description Banana wilt-like disease, triggered by Fusarium oxysporum, poses a severe threat to banana cultivation as a persistent soilborne pathogen. A strategy to effectively control this disease has yet to be found. Dark septate endophytes (DSEs) fungi have emerged as promising biocontrol agents, not only offering protection against plant pathogens but also enhancing plant growth. Herein, we isolated a novel DSEs species, Acidomelania saccharicola LZ3, from sugarcane (Saccharum officinarum) soil in Liuzhou, Guangxi Province, China. Its classification as a new species was confirmed through morphological characterization and DNA sequence analysis. A. saccharicola LZ3 promoted the growth of banana and inhibited the growth of F. oxysporum by 73.28% in a Petri dish assay, and reduced disease by 52.82% in pots. The average incidence rate of Banana-LZ3 symbionts transplanted into a field highly contaminated with F. oxysporum was 25.93% and the control effect was 53.27%. Inoculation of banana plants with A. saccharicola LZ3 significantly increased polyphenol oxidase (POD) and superoxide dismutase (SOD) activity. This research lays the groundwork for future investigations into A. saccharicola and other DSEs, exploring their potential in sustainable plant disease management.
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spelling doaj-art-d3441c630e7e408cb8e4a926b637f22b2025-08-20T02:31:00ZengElsevierBiological Control1049-96442025-07-0120610578110.1016/j.biocontrol.2025.105781Acidomelania saccharicola sp. nov., a new species of dark septate endophytes in Helotiales, with potential of controlling Fusarium wilt of bananaQian Nong0Yan Zhang1Yanyan Long2Yanlu Chen3Liping Qin4Shanyu Lin5Fenghua Zeng6Ling Xie7Plant Protection Research Institute of Guangxi Academy of Agricultural Sciences, Nanning 530007 Guangxi, China; Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pest, Nanning 530007 Guangxi, China; Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs, Nanning 530007 Guangxi, ChinaPlant Protection Research Institute of Guangxi Academy of Agricultural Sciences, Nanning 530007 Guangxi, China; Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pest, Nanning 530007 Guangxi, China; Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs, Nanning 530007 Guangxi, ChinaPlant Protection Research Institute of Guangxi Academy of Agricultural Sciences, Nanning 530007 Guangxi, China; Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pest, Nanning 530007 Guangxi, China; Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs, Nanning 530007 Guangxi, ChinaInstitute of Biological Sciences and Technology, Guangxi Academy of Sciences, Nanning 530007 Guangxi, ChinaPlant Protection Research Institute of Guangxi Academy of Agricultural Sciences, Nanning 530007 Guangxi, China; Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pest, Nanning 530007 Guangxi, China; Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs, Nanning 530007 Guangxi, ChinaPlant Protection Research Institute of Guangxi Academy of Agricultural Sciences, Nanning 530007 Guangxi, China; Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pest, Nanning 530007 Guangxi, China; Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs, Nanning 530007 Guangxi, ChinaPlant Protection Research Institute of Guangxi Academy of Agricultural Sciences, Nanning 530007 Guangxi, China; Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pest, Nanning 530007 Guangxi, China; Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs, Nanning 530007 Guangxi, China; Corresponding authors.Plant Protection Research Institute of Guangxi Academy of Agricultural Sciences, Nanning 530007 Guangxi, China; Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pest, Nanning 530007 Guangxi, China; Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs, Nanning 530007 Guangxi, China; Corresponding authors.Banana wilt-like disease, triggered by Fusarium oxysporum, poses a severe threat to banana cultivation as a persistent soilborne pathogen. A strategy to effectively control this disease has yet to be found. Dark septate endophytes (DSEs) fungi have emerged as promising biocontrol agents, not only offering protection against plant pathogens but also enhancing plant growth. Herein, we isolated a novel DSEs species, Acidomelania saccharicola LZ3, from sugarcane (Saccharum officinarum) soil in Liuzhou, Guangxi Province, China. Its classification as a new species was confirmed through morphological characterization and DNA sequence analysis. A. saccharicola LZ3 promoted the growth of banana and inhibited the growth of F. oxysporum by 73.28% in a Petri dish assay, and reduced disease by 52.82% in pots. The average incidence rate of Banana-LZ3 symbionts transplanted into a field highly contaminated with F. oxysporum was 25.93% and the control effect was 53.27%. Inoculation of banana plants with A. saccharicola LZ3 significantly increased polyphenol oxidase (POD) and superoxide dismutase (SOD) activity. This research lays the groundwork for future investigations into A. saccharicola and other DSEs, exploring their potential in sustainable plant disease management.http://www.sciencedirect.com/science/article/pii/S104996442500091XAcidomelaniaFungal morphologyPhylogenetic analysisFusarium wiltBiological disease controlSaccharum officinarum
spellingShingle Qian Nong
Yan Zhang
Yanyan Long
Yanlu Chen
Liping Qin
Shanyu Lin
Fenghua Zeng
Ling Xie
Acidomelania saccharicola sp. nov., a new species of dark septate endophytes in Helotiales, with potential of controlling Fusarium wilt of banana
Biological Control
Acidomelania
Fungal morphology
Phylogenetic analysis
Fusarium wilt
Biological disease control
Saccharum officinarum
title Acidomelania saccharicola sp. nov., a new species of dark septate endophytes in Helotiales, with potential of controlling Fusarium wilt of banana
title_full Acidomelania saccharicola sp. nov., a new species of dark septate endophytes in Helotiales, with potential of controlling Fusarium wilt of banana
title_fullStr Acidomelania saccharicola sp. nov., a new species of dark septate endophytes in Helotiales, with potential of controlling Fusarium wilt of banana
title_full_unstemmed Acidomelania saccharicola sp. nov., a new species of dark septate endophytes in Helotiales, with potential of controlling Fusarium wilt of banana
title_short Acidomelania saccharicola sp. nov., a new species of dark septate endophytes in Helotiales, with potential of controlling Fusarium wilt of banana
title_sort acidomelania saccharicola sp nov a new species of dark septate endophytes in helotiales with potential of controlling fusarium wilt of banana
topic Acidomelania
Fungal morphology
Phylogenetic analysis
Fusarium wilt
Biological disease control
Saccharum officinarum
url http://www.sciencedirect.com/science/article/pii/S104996442500091X
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