Effect of Lmo7 on proliferation, migration and osteogenic differentiation of MC3T3-E1 cells in vitro
[Objective:] To investigate the effects of Lmo7 gene in the process of proliferation, migration and osteogenic differentiation of MC3T3-E1 cells. [Methods:] The preosteoblasts MC3T3-E1 cells were cultured in vitro with osteogenic induction. The relative expression of Lmo7 was detected by real-time q...
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Editorial Office of Journal of Oral and Maxillofacial Surgery
2023-12-01
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| Series: | Kouqiang hemian waike zazhi |
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| Online Access: | https://journal06.magtech.org.cn/Jweb_joms/EN/10.12439/kqhm.1005-4979.2023.06.001 |
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| _version_ | 1849225133728727040 |
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| author | MAO Jiayi WANG Zuolin |
| author_facet | MAO Jiayi WANG Zuolin |
| author_sort | MAO Jiayi |
| collection | DOAJ |
| description | [Objective:] To investigate the effects of Lmo7 gene in the process of proliferation, migration and osteogenic differentiation of MC3T3-E1 cells. [Methods:] The preosteoblasts MC3T3-E1 cells were cultured in vitro with osteogenic induction. The relative expression of Lmo7 was detected by real-time quantitative polymerase chain reaction (RT-qPCR) and Western blotting at 0, 3, 7 and 14 d respectively. At the same time, MC3T3-E1 cells were transfected with pLV-shLmo7 and pLV-shControl to construct the stably interfering cell line. Cell proliferation and cell migration were detected by CCK-8 assay, EdU assay, wound healing assay and Transwell migration assay. While alkaline phosphatase (ALP) staining was used to detect the expression activity of ALP, and RT-qPCR was used to detect the expression of osteogenesis-related genes such as Runt-related transcription factor 2 (Runx2), Osterix, alkaline phosphatase (ALP) and osteocalcin (OCN). [Results:] Lmo7 gene expression level was up-regulated during the osteogenic differentiation of MC3T3-E1 cells in vitro. The stable MC3T3-E1 cell line with Lmo7 interference was successfully constructed, and its proliferation activity was significantly improved, while its migration ability was inhibited. After osteogenic induction, compared with the control group, the ALP staining of the Lmo7 interfering cells was lighter, and the expression levels of osteogenesis-related genes such as Runx2, Osterix, ALP, and OCN were significantly down-regulated (P<0.05). [Conclusion:] Lmo7 interference promoted the cell proliferation but inhibited the cell migration. Furthermore, the osteogenic differentiation ability of cells was suppressed. |
| format | Article |
| id | doaj-art-d3379257a4a242c080539868efd2fe35 |
| institution | Kabale University |
| issn | 1005-4979 |
| language | zho |
| publishDate | 2023-12-01 |
| publisher | Editorial Office of Journal of Oral and Maxillofacial Surgery |
| record_format | Article |
| series | Kouqiang hemian waike zazhi |
| spelling | doaj-art-d3379257a4a242c080539868efd2fe352025-08-25T06:10:25ZzhoEditorial Office of Journal of Oral and Maxillofacial SurgeryKouqiang hemian waike zazhi1005-49792023-12-0133635536210.12439/kqhm.1005-4979.2023.06.001Effect of Lmo7 on proliferation, migration and osteogenic differentiation of MC3T3-E1 cells in vitroMAO Jiayi0WANG Zuolin1Department of Oral Implantology, Stomatological Hospital and Dental School of Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai 200072, ChinaDepartment of Oral Implantology, Stomatological Hospital and Dental School of Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai 200072, China[Objective:] To investigate the effects of Lmo7 gene in the process of proliferation, migration and osteogenic differentiation of MC3T3-E1 cells. [Methods:] The preosteoblasts MC3T3-E1 cells were cultured in vitro with osteogenic induction. The relative expression of Lmo7 was detected by real-time quantitative polymerase chain reaction (RT-qPCR) and Western blotting at 0, 3, 7 and 14 d respectively. At the same time, MC3T3-E1 cells were transfected with pLV-shLmo7 and pLV-shControl to construct the stably interfering cell line. Cell proliferation and cell migration were detected by CCK-8 assay, EdU assay, wound healing assay and Transwell migration assay. While alkaline phosphatase (ALP) staining was used to detect the expression activity of ALP, and RT-qPCR was used to detect the expression of osteogenesis-related genes such as Runt-related transcription factor 2 (Runx2), Osterix, alkaline phosphatase (ALP) and osteocalcin (OCN). [Results:] Lmo7 gene expression level was up-regulated during the osteogenic differentiation of MC3T3-E1 cells in vitro. The stable MC3T3-E1 cell line with Lmo7 interference was successfully constructed, and its proliferation activity was significantly improved, while its migration ability was inhibited. After osteogenic induction, compared with the control group, the ALP staining of the Lmo7 interfering cells was lighter, and the expression levels of osteogenesis-related genes such as Runx2, Osterix, ALP, and OCN were significantly down-regulated (P<0.05). [Conclusion:] Lmo7 interference promoted the cell proliferation but inhibited the cell migration. Furthermore, the osteogenic differentiation ability of cells was suppressed.https://journal06.magtech.org.cn/Jweb_joms/EN/10.12439/kqhm.1005-4979.2023.06.001lmo7mc3t3-e1 cellsrna interference |
| spellingShingle | MAO Jiayi WANG Zuolin Effect of Lmo7 on proliferation, migration and osteogenic differentiation of MC3T3-E1 cells in vitro Kouqiang hemian waike zazhi lmo7 mc3t3-e1 cells rna interference |
| title | Effect of Lmo7 on proliferation, migration and osteogenic differentiation of MC3T3-E1 cells in vitro |
| title_full | Effect of Lmo7 on proliferation, migration and osteogenic differentiation of MC3T3-E1 cells in vitro |
| title_fullStr | Effect of Lmo7 on proliferation, migration and osteogenic differentiation of MC3T3-E1 cells in vitro |
| title_full_unstemmed | Effect of Lmo7 on proliferation, migration and osteogenic differentiation of MC3T3-E1 cells in vitro |
| title_short | Effect of Lmo7 on proliferation, migration and osteogenic differentiation of MC3T3-E1 cells in vitro |
| title_sort | effect of lmo7 on proliferation migration and osteogenic differentiation of mc3t3 e1 cells in vitro |
| topic | lmo7 mc3t3-e1 cells rna interference |
| url | https://journal06.magtech.org.cn/Jweb_joms/EN/10.12439/kqhm.1005-4979.2023.06.001 |
| work_keys_str_mv | AT maojiayi effectoflmo7onproliferationmigrationandosteogenicdifferentiationofmc3t3e1cellsinvitro AT wangzuolin effectoflmo7onproliferationmigrationandosteogenicdifferentiationofmc3t3e1cellsinvitro |