Field-ready biomarkers for quantifying dehydration: applications for mountain and high-altitude warfighters

Background Dehydration is a critical threat to performance and safety in tactical settings, particularly during prolonged activity in heat or altitude while wearing heavy gear. Sweat-prone regions like the chest, back, and lower legs, which are areas typically covered by packs, armor, and boots, may...

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Main Authors: S. Kyle Travis, Antonella Schwarz
Format: Article
Language:English
Published: Taylor & Francis Group 2025-09-01
Series:Journal of the International Society of Sports Nutrition
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Online Access:https://www.tandfonline.com/doi/10.1080/15502783.2025.2550166
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author S. Kyle Travis
Antonella Schwarz
author_facet S. Kyle Travis
Antonella Schwarz
author_sort S. Kyle Travis
collection DOAJ
description Background Dehydration is a critical threat to performance and safety in tactical settings, particularly during prolonged activity in heat or altitude while wearing heavy gear. Sweat-prone regions like the chest, back, and lower legs, which are areas typically covered by packs, armor, and boots, may experience accelerated fluid loss. This study evaluated whether noninvasive salivary biomarkers and regional skin temperatures could serve as practical indicators of hydration status.Methods Ten males (25.5 ± 3.5 yrs, 179.9 ± 7.2 cm, 78.1 ± 8.5 kg) completed a 2-hour treadmill run without fluid intake. Saliva and skin temperature measurements were collected every 15 minutes (8 timepoints). Biomarkers (osmolality, chloride, and cortisol) and regional skin temperatures (chest, back, lower leg) were analyzed relative to dehydration. Total body water (TBW) loss and percent dehydration were used as outcomes. Bayesian Pearson correlations (r) and Bayes Factors (BF10) assessed associations, with magnitude and evidence strength classified using Hopkins and Jeffreys scales. Bayesian multiple regression models (R2) evaluated predictive contributions of each variable, with model outputs including Bayes factor inclusion scores (BFincl) and standardized beta coefficients (ß). Analyses were performed in JASP (v0.19.3).Results Salivary osmolality (BF10 = 58.00; r = –0.142) and chloride (BF10 = 53.25; r = –0.010) were supported by very strong evidence as predictors of total body water (TBW) loss, though both exhibited small, inverse associations with TBW loss. Cortisol (BF10 = 23.55; r = 0.311) exhibited strong evidence and a moderate positive correlation, increasing as TBW loss increased. Chest temperature demonstrated the strongest predictive contribution in the regression model (BFincl  = 1874.60; ß = –0.167), followed by lower leg (BFincl  = 45.82; ß = –0.153) and back (BFincl  = 34.81; ß = –0.145). Negative beta coefficients reflected positive associations between rising surface temperatures and greater dehydration. Fluid-based models explained up to 70.6% of TBW loss variance (R2 = 0.706), outperforming temperature-only models (R2 = 0.451). When models were replicated using percent dehydration as the outcome, results remained consistent; chest temperature remained the most consistently included thermal predictor (BF10 = 7.34), though the strength of its association was small.Conclusions Salivary osmolality, chloride, and cortisol, along with chest, back, and lower leg temperature, show strong promise as noninvasive, field-ready indicators of dehydration. These findings support the use of portable thermal sensors and saliva-based tools to help tactical personnel monitor hydration status during sustained operations in austere environments.
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spelling doaj-art-d32fe555366d4df59bd71d00b5b3f7db2025-08-26T10:43:11ZengTaylor & Francis GroupJournal of the International Society of Sports Nutrition1550-27832025-09-0122sup210.1080/15502783.2025.2550166Field-ready biomarkers for quantifying dehydration: applications for mountain and high-altitude warfightersS. Kyle Travis0Antonella Schwarz1Liberty University, School of Health Sciences, Department of Allied Health Professions, Lynchburg, VA, USABarry University, Department of Sport and Exercise Sciences, Miami Shores, FL, USABackground Dehydration is a critical threat to performance and safety in tactical settings, particularly during prolonged activity in heat or altitude while wearing heavy gear. Sweat-prone regions like the chest, back, and lower legs, which are areas typically covered by packs, armor, and boots, may experience accelerated fluid loss. This study evaluated whether noninvasive salivary biomarkers and regional skin temperatures could serve as practical indicators of hydration status.Methods Ten males (25.5 ± 3.5 yrs, 179.9 ± 7.2 cm, 78.1 ± 8.5 kg) completed a 2-hour treadmill run without fluid intake. Saliva and skin temperature measurements were collected every 15 minutes (8 timepoints). Biomarkers (osmolality, chloride, and cortisol) and regional skin temperatures (chest, back, lower leg) were analyzed relative to dehydration. Total body water (TBW) loss and percent dehydration were used as outcomes. Bayesian Pearson correlations (r) and Bayes Factors (BF10) assessed associations, with magnitude and evidence strength classified using Hopkins and Jeffreys scales. Bayesian multiple regression models (R2) evaluated predictive contributions of each variable, with model outputs including Bayes factor inclusion scores (BFincl) and standardized beta coefficients (ß). Analyses were performed in JASP (v0.19.3).Results Salivary osmolality (BF10 = 58.00; r = –0.142) and chloride (BF10 = 53.25; r = –0.010) were supported by very strong evidence as predictors of total body water (TBW) loss, though both exhibited small, inverse associations with TBW loss. Cortisol (BF10 = 23.55; r = 0.311) exhibited strong evidence and a moderate positive correlation, increasing as TBW loss increased. Chest temperature demonstrated the strongest predictive contribution in the regression model (BFincl  = 1874.60; ß = –0.167), followed by lower leg (BFincl  = 45.82; ß = –0.153) and back (BFincl  = 34.81; ß = –0.145). Negative beta coefficients reflected positive associations between rising surface temperatures and greater dehydration. Fluid-based models explained up to 70.6% of TBW loss variance (R2 = 0.706), outperforming temperature-only models (R2 = 0.451). When models were replicated using percent dehydration as the outcome, results remained consistent; chest temperature remained the most consistently included thermal predictor (BF10 = 7.34), though the strength of its association was small.Conclusions Salivary osmolality, chloride, and cortisol, along with chest, back, and lower leg temperature, show strong promise as noninvasive, field-ready indicators of dehydration. These findings support the use of portable thermal sensors and saliva-based tools to help tactical personnel monitor hydration status during sustained operations in austere environments.https://www.tandfonline.com/doi/10.1080/15502783.2025.2550166Tacticalhydrationnoninvasivefluid loss
spellingShingle S. Kyle Travis
Antonella Schwarz
Field-ready biomarkers for quantifying dehydration: applications for mountain and high-altitude warfighters
Journal of the International Society of Sports Nutrition
Tactical
hydration
noninvasive
fluid loss
title Field-ready biomarkers for quantifying dehydration: applications for mountain and high-altitude warfighters
title_full Field-ready biomarkers for quantifying dehydration: applications for mountain and high-altitude warfighters
title_fullStr Field-ready biomarkers for quantifying dehydration: applications for mountain and high-altitude warfighters
title_full_unstemmed Field-ready biomarkers for quantifying dehydration: applications for mountain and high-altitude warfighters
title_short Field-ready biomarkers for quantifying dehydration: applications for mountain and high-altitude warfighters
title_sort field ready biomarkers for quantifying dehydration applications for mountain and high altitude warfighters
topic Tactical
hydration
noninvasive
fluid loss
url https://www.tandfonline.com/doi/10.1080/15502783.2025.2550166
work_keys_str_mv AT skyletravis fieldreadybiomarkersforquantifyingdehydrationapplicationsformountainandhighaltitudewarfighters
AT antonellaschwarz fieldreadybiomarkersforquantifyingdehydrationapplicationsformountainandhighaltitudewarfighters