Determination of Lipoxygenase Activity in Cereals Grains Using a Silver Nanoparticles Assay
A spectrophotometric method based on the use of silver nanoparticles is presented for the determination of lipoxygenase activity in cereal grains. The method involves the synthesis of prism silver nanoparticles with a maximum absorption at 752 nm, followed by the abatement of the signal as a consequ...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2015-01-01
|
| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2015/786430 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832562566526664704 |
|---|---|
| author | Raybel Muñoz Jose A. Rodriguez M. Elena Páez-Hernández Irais Sánchez-Ortega Eva M. Santos |
| author_facet | Raybel Muñoz Jose A. Rodriguez M. Elena Páez-Hernández Irais Sánchez-Ortega Eva M. Santos |
| author_sort | Raybel Muñoz |
| collection | DOAJ |
| description | A spectrophotometric method based on the use of silver nanoparticles is presented for the determination of lipoxygenase activity in cereal grains. The method involves the synthesis of prism silver nanoparticles with a maximum absorption at 752 nm, followed by the abatement of the signal as a consequence of the oxidation produced by hydroperoxides generated in the enzymatic reaction. The quantification of the hydroperoxides produced by reaction of lipoxygenase with linoleic acid allows the determination of the enzyme activity in cereal grains. Under optimal conditions, the linear range of the calibration curve ranges from 1.0 to 5.0 μM, with a limit of detection of 0.34 μM. The method was validated comparing the results with those obtained by ferrous-xylenol orange method. A relative standard deviation < 5.0% was obtained in all cases and no significant differences were observed (p<0.05). |
| format | Article |
| id | doaj-art-d32a8a692bb94801b7ddc9c57a826fe8 |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-d32a8a692bb94801b7ddc9c57a826fe82025-02-03T01:22:21ZengWileyJournal of Chemistry2090-90632090-90712015-01-01201510.1155/2015/786430786430Determination of Lipoxygenase Activity in Cereals Grains Using a Silver Nanoparticles AssayRaybel Muñoz0Jose A. Rodriguez1M. Elena Páez-Hernández2Irais Sánchez-Ortega3Eva M. Santos4Área Académica de Química, Universidad Autónoma del Estado de Hidalgo, Carretera Pachuca-Tulancingo Km. 4.5, 42184 Mineral de la Reforma, HGO, MexicoÁrea Académica de Química, Universidad Autónoma del Estado de Hidalgo, Carretera Pachuca-Tulancingo Km. 4.5, 42184 Mineral de la Reforma, HGO, MexicoÁrea Académica de Química, Universidad Autónoma del Estado de Hidalgo, Carretera Pachuca-Tulancingo Km. 4.5, 42184 Mineral de la Reforma, HGO, MexicoÁrea Académica de Química, Universidad Autónoma del Estado de Hidalgo, Carretera Pachuca-Tulancingo Km. 4.5, 42184 Mineral de la Reforma, HGO, MexicoÁrea Académica de Química, Universidad Autónoma del Estado de Hidalgo, Carretera Pachuca-Tulancingo Km. 4.5, 42184 Mineral de la Reforma, HGO, MexicoA spectrophotometric method based on the use of silver nanoparticles is presented for the determination of lipoxygenase activity in cereal grains. The method involves the synthesis of prism silver nanoparticles with a maximum absorption at 752 nm, followed by the abatement of the signal as a consequence of the oxidation produced by hydroperoxides generated in the enzymatic reaction. The quantification of the hydroperoxides produced by reaction of lipoxygenase with linoleic acid allows the determination of the enzyme activity in cereal grains. Under optimal conditions, the linear range of the calibration curve ranges from 1.0 to 5.0 μM, with a limit of detection of 0.34 μM. The method was validated comparing the results with those obtained by ferrous-xylenol orange method. A relative standard deviation < 5.0% was obtained in all cases and no significant differences were observed (p<0.05).http://dx.doi.org/10.1155/2015/786430 |
| spellingShingle | Raybel Muñoz Jose A. Rodriguez M. Elena Páez-Hernández Irais Sánchez-Ortega Eva M. Santos Determination of Lipoxygenase Activity in Cereals Grains Using a Silver Nanoparticles Assay Journal of Chemistry |
| title | Determination of Lipoxygenase Activity in Cereals Grains Using a Silver Nanoparticles Assay |
| title_full | Determination of Lipoxygenase Activity in Cereals Grains Using a Silver Nanoparticles Assay |
| title_fullStr | Determination of Lipoxygenase Activity in Cereals Grains Using a Silver Nanoparticles Assay |
| title_full_unstemmed | Determination of Lipoxygenase Activity in Cereals Grains Using a Silver Nanoparticles Assay |
| title_short | Determination of Lipoxygenase Activity in Cereals Grains Using a Silver Nanoparticles Assay |
| title_sort | determination of lipoxygenase activity in cereals grains using a silver nanoparticles assay |
| url | http://dx.doi.org/10.1155/2015/786430 |
| work_keys_str_mv | AT raybelmunoz determinationoflipoxygenaseactivityincerealsgrainsusingasilvernanoparticlesassay AT josearodriguez determinationoflipoxygenaseactivityincerealsgrainsusingasilvernanoparticlesassay AT melenapaezhernandez determinationoflipoxygenaseactivityincerealsgrainsusingasilvernanoparticlesassay AT iraissanchezortega determinationoflipoxygenaseactivityincerealsgrainsusingasilvernanoparticlesassay AT evamsantos determinationoflipoxygenaseactivityincerealsgrainsusingasilvernanoparticlesassay |