APPLICATION OF SILICA RICE HUSK ASH FOR CELLULASE IMMOBILIZED BY SOL-GEL ENTRAPMENT
Biosilica from rice husk ash can be used to immobilize enzyme by sol-gel entrapment method. Cellulase was chosen as the probe enzyme to test the feasibility of this material for such purpose. This research aimed to know the optimum condition of cellulase immobilization process by sol-gel entrapm...
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| Format: | Article |
| Language: | English |
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Alma Mater Publishing House "Vasile Alecsandri" University of Bacau
2021-03-01
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| Series: | Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry |
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| Online Access: | http://pubs.ub.ro/?pg=revues&rev=cscc6&num=202101&vol=1&aid=5248 |
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| author | ANTONIUS DIONOVTA R. P. MOLO EVI SUSANTI SURJANI WONORAHARDJO |
| author_facet | ANTONIUS DIONOVTA R. P. MOLO EVI SUSANTI SURJANI WONORAHARDJO |
| author_sort | ANTONIUS DIONOVTA R. P. MOLO |
| collection | DOAJ |
| description | Biosilica from rice husk ash can be used to immobilize
enzyme by sol-gel entrapment method. Cellulase was chosen as the probe
enzyme to test the feasibility of this material for such purpose. This research
aimed to know the optimum condition of cellulase immobilization process
by sol-gel entrapment, the optimum pH and temperature for immobilized
enzyme, and the reusability of the immobilized enzyme, as concluded from
its activity. The research was divided into 3 steps (1) sol-gel immobilization
of cellulase, (2) protein and activity experiments to test immobilized
enzyme by varying pH and temperature, and (3) reusability test. The result
showed optimum condition gave 38.48 % recovery of enzyme activity with
protein content of 0.42 mg·mL-1, the best pH and temperature were 7 and
55 C respectively. The system could be reused for three times. |
| format | Article |
| id | doaj-art-d31ee1cc5cd84b358dc0c590c81b2c24 |
| institution | OA Journals |
| issn | 1582-540X 1582-540X |
| language | English |
| publishDate | 2021-03-01 |
| publisher | Alma Mater Publishing House "Vasile Alecsandri" University of Bacau |
| record_format | Article |
| series | Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry |
| spelling | doaj-art-d31ee1cc5cd84b358dc0c590c81b2c242025-08-20T02:15:47ZengAlma Mater Publishing House "Vasile Alecsandri" University of BacauScientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry1582-540X1582-540X2021-03-012214755APPLICATION OF SILICA RICE HUSK ASH FOR CELLULASE IMMOBILIZED BY SOL-GEL ENTRAPMENT ANTONIUS DIONOVTA R. P. MOLO 0 EVI SUSANTI 1SURJANI WONORAHARDJO 21. Department of Chemistry, Faculty of Mathematic and Natural Science, Universitas Negeri Ma ang, Indonesia 1. Department of Chemistry, Faculty of Mathematic and Natural Science, Universitas Negeri Ma ang, Indonesia 1. Department of Chemistry, Faculty of Mathematic and Natural Science, Universitas Negeri Ma ang, Indonesia 2. Center of Advanced Material and Renewable Energy (CAMRY), State University of Malang, IndonesiaBiosilica from rice husk ash can be used to immobilize enzyme by sol-gel entrapment method. Cellulase was chosen as the probe enzyme to test the feasibility of this material for such purpose. This research aimed to know the optimum condition of cellulase immobilization process by sol-gel entrapment, the optimum pH and temperature for immobilized enzyme, and the reusability of the immobilized enzyme, as concluded from its activity. The research was divided into 3 steps (1) sol-gel immobilization of cellulase, (2) protein and activity experiments to test immobilized enzyme by varying pH and temperature, and (3) reusability test. The result showed optimum condition gave 38.48 % recovery of enzyme activity with protein content of 0.42 mg·mL-1, the best pH and temperature were 7 and 55 C respectively. The system could be reused for three times.http://pubs.ub.ro/?pg=revues&rev=cscc6&num=202101&vol=1&aid=5248biomaterialenzymeimmobilizationoptimum conditionreusability |
| spellingShingle | ANTONIUS DIONOVTA R. P. MOLO EVI SUSANTI SURJANI WONORAHARDJO APPLICATION OF SILICA RICE HUSK ASH FOR CELLULASE IMMOBILIZED BY SOL-GEL ENTRAPMENT Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry biomaterial enzyme immobilization optimum condition reusability |
| title | APPLICATION OF SILICA RICE HUSK ASH FOR CELLULASE IMMOBILIZED BY SOL-GEL ENTRAPMENT |
| title_full | APPLICATION OF SILICA RICE HUSK ASH FOR CELLULASE IMMOBILIZED BY SOL-GEL ENTRAPMENT |
| title_fullStr | APPLICATION OF SILICA RICE HUSK ASH FOR CELLULASE IMMOBILIZED BY SOL-GEL ENTRAPMENT |
| title_full_unstemmed | APPLICATION OF SILICA RICE HUSK ASH FOR CELLULASE IMMOBILIZED BY SOL-GEL ENTRAPMENT |
| title_short | APPLICATION OF SILICA RICE HUSK ASH FOR CELLULASE IMMOBILIZED BY SOL-GEL ENTRAPMENT |
| title_sort | application of silica rice husk ash for cellulase immobilized by sol gel entrapment |
| topic | biomaterial enzyme immobilization optimum condition reusability |
| url | http://pubs.ub.ro/?pg=revues&rev=cscc6&num=202101&vol=1&aid=5248 |
| work_keys_str_mv | AT antoniusdionovtarpmolo applicationofsilicaricehuskashforcellulaseimmobilizedbysolgelentrapment AT evisusanti applicationofsilicaricehuskashforcellulaseimmobilizedbysolgelentrapment AT surjaniwonorahardjo applicationofsilicaricehuskashforcellulaseimmobilizedbysolgelentrapment |