APPLICATION OF SILICA RICE HUSK ASH FOR CELLULASE IMMOBILIZED BY SOL-GEL ENTRAPMENT
Biosilica from rice husk ash can be used to immobilize enzyme by sol-gel entrapment method. Cellulase was chosen as the probe enzyme to test the feasibility of this material for such purpose. This research aimed to know the optimum condition of cellulase immobilization process by sol-gel entrapm...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Alma Mater Publishing House "Vasile Alecsandri" University of Bacau
2021-03-01
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| Series: | Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry |
| Subjects: | |
| Online Access: | http://pubs.ub.ro/?pg=revues&rev=cscc6&num=202101&vol=1&aid=5248 |
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| Summary: | Biosilica from rice husk ash can be used to immobilize
enzyme by sol-gel entrapment method. Cellulase was chosen as the probe
enzyme to test the feasibility of this material for such purpose. This research
aimed to know the optimum condition of cellulase immobilization process
by sol-gel entrapment, the optimum pH and temperature for immobilized
enzyme, and the reusability of the immobilized enzyme, as concluded from
its activity. The research was divided into 3 steps (1) sol-gel immobilization
of cellulase, (2) protein and activity experiments to test immobilized
enzyme by varying pH and temperature, and (3) reusability test. The result
showed optimum condition gave 38.48 % recovery of enzyme activity with
protein content of 0.42 mg·mL-1, the best pH and temperature were 7 and
55 C respectively. The system could be reused for three times. |
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| ISSN: | 1582-540X 1582-540X |