Preparation of Magnetic Molecularly Imprinted Polymers for Selective Recognition and Determination of Clenbuterol in Pork Samples

Magnetic molecularly imprinted polymer (MMIP) was successfully synthesized with acrylamide as a functional monomer and clenbuterol (CLB) as a template molecule. The synthesized MMIPs were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier transform infrared spe...

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Main Authors: Yingjuan Zhao, Zufei Feng, Sijin Li, Yan Lu, Yangfan Hu, Hua Fan, Xinyue Zhai
Format: Article
Language:English
Published: Wiley 2020-01-01
Series:Journal of Chemistry
Online Access:http://dx.doi.org/10.1155/2020/8820262
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author Yingjuan Zhao
Zufei Feng
Sijin Li
Yan Lu
Yangfan Hu
Hua Fan
Xinyue Zhai
author_facet Yingjuan Zhao
Zufei Feng
Sijin Li
Yan Lu
Yangfan Hu
Hua Fan
Xinyue Zhai
author_sort Yingjuan Zhao
collection DOAJ
description Magnetic molecularly imprinted polymer (MMIP) was successfully synthesized with acrylamide as a functional monomer and clenbuterol (CLB) as a template molecule. The synthesized MMIPs were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier transform infrared spectrometry (FT-IR). MMIPs were used to identify and bind CLB as a solid phase extraction material. The experiment data were fitted by the Freundlich isotherm adsorption model. The results show that MMIPs have excellent recognition performance for CLB. MMIPs were successfully applied as adsorbents to preconcentrated CLB in pork samples and detected by HPLC with UV. The limit of detection (LOD) and limit of quantification (LOQ) were 4.27 μg/L and 14.2 μg/L, respectively. The spiked recovery rates ranged from 94.44% to 102.29%. Therefore, the prepared MMIPs can be used for selective preconcentration of CLB content in complex animal-derived food samples.
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institution Kabale University
issn 2090-9063
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language English
publishDate 2020-01-01
publisher Wiley
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series Journal of Chemistry
spelling doaj-art-d30ba970a1f04c6d82b831143674ea2e2025-08-20T03:24:07ZengWileyJournal of Chemistry2090-90632090-90712020-01-01202010.1155/2020/88202628820262Preparation of Magnetic Molecularly Imprinted Polymers for Selective Recognition and Determination of Clenbuterol in Pork SamplesYingjuan Zhao0Zufei Feng1Sijin Li2Yan Lu3Yangfan Hu4Hua Fan5Xinyue Zhai6Department of Applied Chemistry, Xi’an University of Technology, Xi’an 710048, ChinaDepartment of Applied Chemistry, Xi’an University of Technology, Xi’an 710048, ChinaDepartment of Applied Chemistry, Xi’an University of Technology, Xi’an 710048, ChinaDepartment of Applied Chemistry, Xi’an University of Technology, Xi’an 710048, ChinaDepartment of Applied Chemistry, Xi’an University of Technology, Xi’an 710048, ChinaDepartment of Applied Chemistry, Xi’an University of Technology, Xi’an 710048, ChinaDepartment of Applied Chemistry, Xi’an University of Technology, Xi’an 710048, ChinaMagnetic molecularly imprinted polymer (MMIP) was successfully synthesized with acrylamide as a functional monomer and clenbuterol (CLB) as a template molecule. The synthesized MMIPs were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier transform infrared spectrometry (FT-IR). MMIPs were used to identify and bind CLB as a solid phase extraction material. The experiment data were fitted by the Freundlich isotherm adsorption model. The results show that MMIPs have excellent recognition performance for CLB. MMIPs were successfully applied as adsorbents to preconcentrated CLB in pork samples and detected by HPLC with UV. The limit of detection (LOD) and limit of quantification (LOQ) were 4.27 μg/L and 14.2 μg/L, respectively. The spiked recovery rates ranged from 94.44% to 102.29%. Therefore, the prepared MMIPs can be used for selective preconcentration of CLB content in complex animal-derived food samples.http://dx.doi.org/10.1155/2020/8820262
spellingShingle Yingjuan Zhao
Zufei Feng
Sijin Li
Yan Lu
Yangfan Hu
Hua Fan
Xinyue Zhai
Preparation of Magnetic Molecularly Imprinted Polymers for Selective Recognition and Determination of Clenbuterol in Pork Samples
Journal of Chemistry
title Preparation of Magnetic Molecularly Imprinted Polymers for Selective Recognition and Determination of Clenbuterol in Pork Samples
title_full Preparation of Magnetic Molecularly Imprinted Polymers for Selective Recognition and Determination of Clenbuterol in Pork Samples
title_fullStr Preparation of Magnetic Molecularly Imprinted Polymers for Selective Recognition and Determination of Clenbuterol in Pork Samples
title_full_unstemmed Preparation of Magnetic Molecularly Imprinted Polymers for Selective Recognition and Determination of Clenbuterol in Pork Samples
title_short Preparation of Magnetic Molecularly Imprinted Polymers for Selective Recognition and Determination of Clenbuterol in Pork Samples
title_sort preparation of magnetic molecularly imprinted polymers for selective recognition and determination of clenbuterol in pork samples
url http://dx.doi.org/10.1155/2020/8820262
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