The Small RNA MicF Represses ObgE and SeqA in <i>Escherichia coli</i>
Small regulatory RNAs (sRNA) have been shown to play a large role in the management of stress responses in <i>Escherichia coli</i> and other bacteria. Upon fluctuations in nutrient availability and exposure to antimicrobials and superoxide-generating agents, the MicF sRNA in <i>E....
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MDPI AG
2024-11-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/12/12/2397 |
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| author | Aaron Y. Stibelman Amy Y. Sariles Melissa K. Takahashi |
| author_facet | Aaron Y. Stibelman Amy Y. Sariles Melissa K. Takahashi |
| author_sort | Aaron Y. Stibelman |
| collection | DOAJ |
| description | Small regulatory RNAs (sRNA) have been shown to play a large role in the management of stress responses in <i>Escherichia coli</i> and other bacteria. Upon fluctuations in nutrient availability and exposure to antimicrobials and superoxide-generating agents, the MicF sRNA in <i>E. coli</i> has been shown to regulate a small set of genes involved in the management of membrane permeability. Currently, it is unknown whether MicF acts on other processes to mediate the response to these agents. Using an sRNA interaction prediction tool, we identified genes in <i>E. coli</i> that are potentially regulated by MicF. Through subsequent analysis using a sfGFP-based reporter–gene fusion, we have validated two novel targets of MicF regulation: ObgE, a GTPase crucial for chromosome partitioning, and SeqA, a negative modulator of DNA replication. Importantly, the interaction between MicF and these target mRNAs is contingent upon the presence of the RNA chaperone protein, Hfq. Furthermore, our findings affirm the role of MicF’s conserved 5’ seed pairing region in initiating these regulatory interactions. Our study suggests that, beyond its established role in membrane permeability management, MicF exerts control over chromosome dynamics in response to distinct environmental cues, implicating a more multifaceted regulatory function in bacterial stress adaptation. |
| format | Article |
| id | doaj-art-d30ac853a95648be915d1bcd2fa3f23c |
| institution | DOAJ |
| issn | 2076-2607 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
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| series | Microorganisms |
| spelling | doaj-art-d30ac853a95648be915d1bcd2fa3f23c2025-08-20T02:43:42ZengMDPI AGMicroorganisms2076-26072024-11-011212239710.3390/microorganisms12122397The Small RNA MicF Represses ObgE and SeqA in <i>Escherichia coli</i>Aaron Y. Stibelman0Amy Y. Sariles1Melissa K. Takahashi2Department of Biology, California State University Northridge, Northridge, CA 91330, USADepartment of Biology, California State University Northridge, Northridge, CA 91330, USADepartment of Biology, California State University Northridge, Northridge, CA 91330, USASmall regulatory RNAs (sRNA) have been shown to play a large role in the management of stress responses in <i>Escherichia coli</i> and other bacteria. Upon fluctuations in nutrient availability and exposure to antimicrobials and superoxide-generating agents, the MicF sRNA in <i>E. coli</i> has been shown to regulate a small set of genes involved in the management of membrane permeability. Currently, it is unknown whether MicF acts on other processes to mediate the response to these agents. Using an sRNA interaction prediction tool, we identified genes in <i>E. coli</i> that are potentially regulated by MicF. Through subsequent analysis using a sfGFP-based reporter–gene fusion, we have validated two novel targets of MicF regulation: ObgE, a GTPase crucial for chromosome partitioning, and SeqA, a negative modulator of DNA replication. Importantly, the interaction between MicF and these target mRNAs is contingent upon the presence of the RNA chaperone protein, Hfq. Furthermore, our findings affirm the role of MicF’s conserved 5’ seed pairing region in initiating these regulatory interactions. Our study suggests that, beyond its established role in membrane permeability management, MicF exerts control over chromosome dynamics in response to distinct environmental cues, implicating a more multifaceted regulatory function in bacterial stress adaptation.https://www.mdpi.com/2076-2607/12/12/2397RNAgene regulation<i>Escherichia coli</i> |
| spellingShingle | Aaron Y. Stibelman Amy Y. Sariles Melissa K. Takahashi The Small RNA MicF Represses ObgE and SeqA in <i>Escherichia coli</i> Microorganisms RNA gene regulation <i>Escherichia coli</i> |
| title | The Small RNA MicF Represses ObgE and SeqA in <i>Escherichia coli</i> |
| title_full | The Small RNA MicF Represses ObgE and SeqA in <i>Escherichia coli</i> |
| title_fullStr | The Small RNA MicF Represses ObgE and SeqA in <i>Escherichia coli</i> |
| title_full_unstemmed | The Small RNA MicF Represses ObgE and SeqA in <i>Escherichia coli</i> |
| title_short | The Small RNA MicF Represses ObgE and SeqA in <i>Escherichia coli</i> |
| title_sort | small rna micf represses obge and seqa in i escherichia coli i |
| topic | RNA gene regulation <i>Escherichia coli</i> |
| url | https://www.mdpi.com/2076-2607/12/12/2397 |
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