Splicing defect of StDRO2 intron 1 promotes potato root growth by disturbing auxin transport to adapt to drought stress
The formation of root system architecture (RSA) plays a crucial role in plant growth. OsDRO1 is known to have a function in controlling RSA in rice, however, the role of potato StDRO2, a homolog of rice OsDRO1, in root growth remains unclear. In this study, we obtained potato dro2 mutant lines by Cl...
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KeAi Communications Co., Ltd.
2025-03-01
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| Series: | Horticultural Plant Journal |
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| author | Jianping Zhao Baolin Yao Ziai Peng Xinyue Yang Kuixiu Li Xiaoyan Zhang Haiyan Zhu Xuan Zhou Meixian Wang Lihui Jiang Xie He Yan Liang Xiaoping Zhan Xiaoran Wang Yuliang Dai Yanfen Yang Ao Yang Man Dong Suni Shi Man Lu Yi Zhao Mingyun Shen Liwei Guo Changning Liu Hongji Zhang Decai Yu Yunlong Du |
| author_facet | Jianping Zhao Baolin Yao Ziai Peng Xinyue Yang Kuixiu Li Xiaoyan Zhang Haiyan Zhu Xuan Zhou Meixian Wang Lihui Jiang Xie He Yan Liang Xiaoping Zhan Xiaoran Wang Yuliang Dai Yanfen Yang Ao Yang Man Dong Suni Shi Man Lu Yi Zhao Mingyun Shen Liwei Guo Changning Liu Hongji Zhang Decai Yu Yunlong Du |
| author_sort | Jianping Zhao |
| collection | DOAJ |
| description | The formation of root system architecture (RSA) plays a crucial role in plant growth. OsDRO1 is known to have a function in controlling RSA in rice, however, the role of potato StDRO2, a homolog of rice OsDRO1, in root growth remains unclear. In this study, we obtained potato dro2 mutant lines by Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR-Associated 9 (CRISPR/Cas9)-mediated genome editing system. The mutant lines were generated from a splicing defect of the StDRO2 intron 1, which causes a nonsense mutation in StDRO2. Furthermore, the secondary structure of StDRO2 mRNA analyzed with RNAfold WebServer was altered in the dro2 mutant. Mutation of StDRO2 conveys potato adaptation through changing the RSA via alteration of auxin transport under drought stress. The potato dro2 lines showed higher plant height, longer root length, smaller root growth angle and increased tuber weight than the wild-type. The alteration of RSA was associated with a disturbance of IAA distribution in the dro2 mutant, and the levels of StPIN7 and StPIN10 detected by using real-time PCR were up-regulated in the roots of potato dro2 lines grown under drought stress. Moreover, the microRNAs (miRNAs) PmiREN024536 and PmiREN024486 targeted the StDRO2 gene, and auxin positively and negatively regulated the expression of StDRO2 and the miRNAs PmiREN024536 and PmiREN024486, respectively, in the potato roots. Our data shows that a regulatory network involving auxin, StDRO2, PmiREN024536 and PmiREN024486 can control RSA to convey potato fitness under drought stress. |
| format | Article |
| id | doaj-art-d3076fc666ae4180a910c4c0800ca80d |
| institution | DOAJ |
| issn | 2468-0141 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Horticultural Plant Journal |
| spelling | doaj-art-d3076fc666ae4180a910c4c0800ca80d2025-08-20T02:55:20ZengKeAi Communications Co., Ltd.Horticultural Plant Journal2468-01412025-03-0111270672010.1016/j.hpj.2023.11.003Splicing defect of StDRO2 intron 1 promotes potato root growth by disturbing auxin transport to adapt to drought stressJianping Zhao0Baolin Yao1Ziai Peng2Xinyue Yang3Kuixiu Li4Xiaoyan Zhang5Haiyan Zhu6Xuan Zhou7Meixian Wang8Lihui Jiang9Xie He10Yan Liang11Xiaoping Zhan12Xiaoran Wang13Yuliang Dai14Yanfen Yang15Ao Yang16Man Dong17Suni Shi18Man Lu19Yi Zhao20Mingyun Shen21Liwei Guo22Changning Liu23Hongji Zhang24Decai Yu25Yunlong Du26College of Plant Protection, Yunnan Agricultural University, Kunming, Yunnan 650201, China; Xuanhan County Plant Quarantine Station, Dazhou, Sichuan 635000, ChinaCAS Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Menglun, Mengla, Yunnan 666300, ChinaCollege of Plant Protection, Yunnan Agricultural University, Kunming, Yunnan 650201, ChinaCollege of Plant Protection, Yunnan Agricultural University, Kunming, Yunnan 650201, ChinaCollege of Plant Protection, Yunnan Agricultural University, Kunming, Yunnan 650201, ChinaCollege of Plant Protection, Yunnan Agricultural University, Kunming, Yunnan 650201, ChinaCollege of Plant Protection, Yunnan Agricultural University, Kunming, Yunnan 650201, ChinaCollege of Plant Protection, Yunnan Agricultural University, Kunming, Yunnan 650201, ChinaCollege of Plant Protection, Yunnan Agricultural University, Kunming, Yunnan 650201, ChinaCollege of Plant Protection, Yunnan Agricultural University, Kunming, Yunnan 650201, ChinaCollege of Plant Protection, Yunnan Agricultural University, Kunming, Yunnan 650201, ChinaYuguopu District Agricultural Comprehensive Service Center, Mengzi, Yunnan 661100, ChinaNing'er County Plant Protection and Plant Quarantine Station, Puer, Yunnan 665000, ChinaCollege of Plant Protection, Yunnan Agricultural University, Kunming, Yunnan 650201, ChinaCollege of Plant Protection, Yunnan Agricultural University, Kunming, Yunnan 650201, ChinaCollege of Plant Protection, Yunnan Agricultural University, Kunming, Yunnan 650201, ChinaCollege of Plant Protection, Yunnan Agricultural University, Kunming, Yunnan 650201, ChinaCollege of Plant Protection, Yunnan Agricultural University, Kunming, Yunnan 650201, ChinaCollege of Plant Protection, Yunnan Agricultural University, Kunming, Yunnan 650201, ChinaCollege of Plant Protection, Yunnan Agricultural University, Kunming, Yunnan 650201, ChinaCollege of Plant Protection, Yunnan Agricultural University, Kunming, Yunnan 650201, ChinaCollege of Plant Protection, Yunnan Agricultural University, Kunming, Yunnan 650201, ChinaCollege of Plant Protection, Yunnan Agricultural University, Kunming, Yunnan 650201, China; State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, Yunnan 650201, China; Key Laboratory of Agro-Biodiversity and Pest Management of Education Ministry of China, College of Plant Protection, Yunnan Agricultural University, Kunming, Yunnan 650201, ChinaCAS Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Menglun, Mengla, Yunnan 666300, ChinaCollege of Landscape and Horticulture, Yunnan Agricultural University, Kunming, Yunnan 650201, China; Key Laboratory of Vegetable Biology of Yunnan Province, College of Landscape and Horticulture, Yunnan Agricultural University, Kunming, Yunnan 650201, China; Corresponding authors.College of Plant Protection, Yunnan Agricultural University, Kunming, Yunnan 650201, China; State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, Yunnan 650201, China; Key Laboratory of Agro-Biodiversity and Pest Management of Education Ministry of China, College of Plant Protection, Yunnan Agricultural University, Kunming, Yunnan 650201, China; Corresponding authors.College of Plant Protection, Yunnan Agricultural University, Kunming, Yunnan 650201, China; State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, Yunnan 650201, China; Key Laboratory of Agro-Biodiversity and Pest Management of Education Ministry of China, College of Plant Protection, Yunnan Agricultural University, Kunming, Yunnan 650201, China; Corresponding authors.The formation of root system architecture (RSA) plays a crucial role in plant growth. OsDRO1 is known to have a function in controlling RSA in rice, however, the role of potato StDRO2, a homolog of rice OsDRO1, in root growth remains unclear. In this study, we obtained potato dro2 mutant lines by Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR-Associated 9 (CRISPR/Cas9)-mediated genome editing system. The mutant lines were generated from a splicing defect of the StDRO2 intron 1, which causes a nonsense mutation in StDRO2. Furthermore, the secondary structure of StDRO2 mRNA analyzed with RNAfold WebServer was altered in the dro2 mutant. Mutation of StDRO2 conveys potato adaptation through changing the RSA via alteration of auxin transport under drought stress. The potato dro2 lines showed higher plant height, longer root length, smaller root growth angle and increased tuber weight than the wild-type. The alteration of RSA was associated with a disturbance of IAA distribution in the dro2 mutant, and the levels of StPIN7 and StPIN10 detected by using real-time PCR were up-regulated in the roots of potato dro2 lines grown under drought stress. Moreover, the microRNAs (miRNAs) PmiREN024536 and PmiREN024486 targeted the StDRO2 gene, and auxin positively and negatively regulated the expression of StDRO2 and the miRNAs PmiREN024536 and PmiREN024486, respectively, in the potato roots. Our data shows that a regulatory network involving auxin, StDRO2, PmiREN024536 and PmiREN024486 can control RSA to convey potato fitness under drought stress.http://www.sciencedirect.com/science/article/pii/S2468014124000074PotatoRoot system architectureStDRO2Drought stressAuxinSplicing |
| spellingShingle | Jianping Zhao Baolin Yao Ziai Peng Xinyue Yang Kuixiu Li Xiaoyan Zhang Haiyan Zhu Xuan Zhou Meixian Wang Lihui Jiang Xie He Yan Liang Xiaoping Zhan Xiaoran Wang Yuliang Dai Yanfen Yang Ao Yang Man Dong Suni Shi Man Lu Yi Zhao Mingyun Shen Liwei Guo Changning Liu Hongji Zhang Decai Yu Yunlong Du Splicing defect of StDRO2 intron 1 promotes potato root growth by disturbing auxin transport to adapt to drought stress Horticultural Plant Journal Potato Root system architecture StDRO2 Drought stress Auxin Splicing |
| title | Splicing defect of StDRO2 intron 1 promotes potato root growth by disturbing auxin transport to adapt to drought stress |
| title_full | Splicing defect of StDRO2 intron 1 promotes potato root growth by disturbing auxin transport to adapt to drought stress |
| title_fullStr | Splicing defect of StDRO2 intron 1 promotes potato root growth by disturbing auxin transport to adapt to drought stress |
| title_full_unstemmed | Splicing defect of StDRO2 intron 1 promotes potato root growth by disturbing auxin transport to adapt to drought stress |
| title_short | Splicing defect of StDRO2 intron 1 promotes potato root growth by disturbing auxin transport to adapt to drought stress |
| title_sort | splicing defect of stdro2 intron 1 promotes potato root growth by disturbing auxin transport to adapt to drought stress |
| topic | Potato Root system architecture StDRO2 Drought stress Auxin Splicing |
| url | http://www.sciencedirect.com/science/article/pii/S2468014124000074 |
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