Investigating the plasma electrophoretic protein profile of kids and lambs during the neonatal period: An overview of their immuno-inflammatory status

The study aimed to evaluate how plasma total proteins, their fractions (i.e. albumin, α1-, β-, γ-globulins) and A/G ratio change in lambs and kids over the first month of life. Ten female Maltese kids and 10 female Comisana lambs were enrolled in the study. Starting from the first day after birth, a...

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Main Authors: Francesca Arfuso, Maria Rizzo, Claudia Giannetto, Marilena Briglia, Elisabetta Giudice, Alessandro Zumbo, Anna Assenza, Giuseppe Piccione
Format: Article
Language:English
Published: Elsevier 2024-11-01
Series:Heliyon
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Online Access:http://www.sciencedirect.com/science/article/pii/S2405844024165395
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author Francesca Arfuso
Maria Rizzo
Claudia Giannetto
Marilena Briglia
Elisabetta Giudice
Alessandro Zumbo
Anna Assenza
Giuseppe Piccione
author_facet Francesca Arfuso
Maria Rizzo
Claudia Giannetto
Marilena Briglia
Elisabetta Giudice
Alessandro Zumbo
Anna Assenza
Giuseppe Piccione
author_sort Francesca Arfuso
collection DOAJ
description The study aimed to evaluate how plasma total proteins, their fractions (i.e. albumin, α1-, β-, γ-globulins) and A/G ratio change in lambs and kids over the first month of life. Ten female Maltese kids and 10 female Comisana lambs were enrolled in the study. Starting from the first day after birth, and for a period of 4 weeks, blood samples were taken from each subject. Specifically, for each kid and lamb, blood sampling was performed within 30 min after birth (T0) (pre-colostral), after colostrum intake (T1), and, weekly, for the subsequent 28 days (T7, T14, T21 and T28). The application of two-way analysis of variance for repeated measures (ANOVA) showed the effect of time in the kids and lambs for the values of plasma total proteins, albumin, α1-, β-, γ-globulins and A/G ratio (p < 0.05). A significant species effect was recorded for α1-and β-globulins (p < 0.05). Specifically, kids showed higher α1-globulins levels at T0, T14, T21 and T28, and higher and β-globulins at T21 and T28 than lambs. The variations found in the levels of plasma total proteins and their fractions in kids and lambs could be related to the physiological changes likely to occur in a newborn animal following transition from the uterine environment to the free-living state. The variability on changes found in α1-and β-globulins measured in kid and lamb seems to suggest that the first one would tend to have a higher acute phase response than lamb stressing the difference between these two small ruminant species.
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spelling doaj-art-d2fb3c5fcb0a40a78d761e36178f66bb2025-08-20T02:49:02ZengElsevierHeliyon2405-84402024-11-011022e4050810.1016/j.heliyon.2024.e40508Investigating the plasma electrophoretic protein profile of kids and lambs during the neonatal period: An overview of their immuno-inflammatory statusFrancesca Arfuso0Maria Rizzo1Claudia Giannetto2Marilena Briglia3Elisabetta Giudice4Alessandro Zumbo5Anna Assenza6Giuseppe Piccione7Department of Veterinary Sciences, University of Messina, Viale Giovanni Palatucci, snc, 98168, Messina, ItalyDepartment of Veterinary Sciences, University of Messina, Viale Giovanni Palatucci, snc, 98168, Messina, Italy; Corresponding author.Department of Veterinary Sciences, University of Messina, Viale Giovanni Palatucci, snc, 98168, Messina, ItalyDepartment of Medicine and Surgery “Kore”, University of Enna, 94100Enna, ItalyDepartment of Veterinary Sciences, University of Messina, Viale Giovanni Palatucci, snc, 98168, Messina, ItalyDepartment of Veterinary Sciences, University of Messina, Viale Giovanni Palatucci, snc, 98168, Messina, ItalyDepartment of Veterinary Sciences, University of Messina, Viale Giovanni Palatucci, snc, 98168, Messina, ItalyDepartment of Veterinary Sciences, University of Messina, Viale Giovanni Palatucci, snc, 98168, Messina, ItalyThe study aimed to evaluate how plasma total proteins, their fractions (i.e. albumin, α1-, β-, γ-globulins) and A/G ratio change in lambs and kids over the first month of life. Ten female Maltese kids and 10 female Comisana lambs were enrolled in the study. Starting from the first day after birth, and for a period of 4 weeks, blood samples were taken from each subject. Specifically, for each kid and lamb, blood sampling was performed within 30 min after birth (T0) (pre-colostral), after colostrum intake (T1), and, weekly, for the subsequent 28 days (T7, T14, T21 and T28). The application of two-way analysis of variance for repeated measures (ANOVA) showed the effect of time in the kids and lambs for the values of plasma total proteins, albumin, α1-, β-, γ-globulins and A/G ratio (p < 0.05). A significant species effect was recorded for α1-and β-globulins (p < 0.05). Specifically, kids showed higher α1-globulins levels at T0, T14, T21 and T28, and higher and β-globulins at T21 and T28 than lambs. The variations found in the levels of plasma total proteins and their fractions in kids and lambs could be related to the physiological changes likely to occur in a newborn animal following transition from the uterine environment to the free-living state. The variability on changes found in α1-and β-globulins measured in kid and lamb seems to suggest that the first one would tend to have a higher acute phase response than lamb stressing the difference between these two small ruminant species.http://www.sciencedirect.com/science/article/pii/S2405844024165395Small ruminantsNeonatal periodGlobulinsGoatProteinsSheep
spellingShingle Francesca Arfuso
Maria Rizzo
Claudia Giannetto
Marilena Briglia
Elisabetta Giudice
Alessandro Zumbo
Anna Assenza
Giuseppe Piccione
Investigating the plasma electrophoretic protein profile of kids and lambs during the neonatal period: An overview of their immuno-inflammatory status
Heliyon
Small ruminants
Neonatal period
Globulins
Goat
Proteins
Sheep
title Investigating the plasma electrophoretic protein profile of kids and lambs during the neonatal period: An overview of their immuno-inflammatory status
title_full Investigating the plasma electrophoretic protein profile of kids and lambs during the neonatal period: An overview of their immuno-inflammatory status
title_fullStr Investigating the plasma electrophoretic protein profile of kids and lambs during the neonatal period: An overview of their immuno-inflammatory status
title_full_unstemmed Investigating the plasma electrophoretic protein profile of kids and lambs during the neonatal period: An overview of their immuno-inflammatory status
title_short Investigating the plasma electrophoretic protein profile of kids and lambs during the neonatal period: An overview of their immuno-inflammatory status
title_sort investigating the plasma electrophoretic protein profile of kids and lambs during the neonatal period an overview of their immuno inflammatory status
topic Small ruminants
Neonatal period
Globulins
Goat
Proteins
Sheep
url http://www.sciencedirect.com/science/article/pii/S2405844024165395
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