Comparative growth kinetics and drug susceptibility of Mycobacterium tuberculosis lineages prevalent in Ethiopia: implications for tuberculosis treatment and management
Tuberculosis (TB) remains a global health challenge, with treatment outcomes influenced by the genetic diversity of Mycobacterium tuberculosis (Mtb) strains. This study examines the growth kinetics and drug susceptibility of Mtb strains from different lineages in Ethiopia to understand their impact...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1512580/full |
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| author | Tesfaye Gebreyohannis Hailemariam Tesfaye Gebreyohannis Hailemariam Melaku Tilahun Abay Atnafu Tesfaye Gelanew Tewodros Tariku Gebresilase Mekdes Alemu Tola Abaysew Ayele Shewki Moga Siraj Workineh Shibeshi Kidist Bobosha Liya Wassie Yonas Hirutu Ephrem Engidawork |
| author_facet | Tesfaye Gebreyohannis Hailemariam Tesfaye Gebreyohannis Hailemariam Melaku Tilahun Abay Atnafu Tesfaye Gelanew Tewodros Tariku Gebresilase Mekdes Alemu Tola Abaysew Ayele Shewki Moga Siraj Workineh Shibeshi Kidist Bobosha Liya Wassie Yonas Hirutu Ephrem Engidawork |
| author_sort | Tesfaye Gebreyohannis Hailemariam |
| collection | DOAJ |
| description | Tuberculosis (TB) remains a global health challenge, with treatment outcomes influenced by the genetic diversity of Mycobacterium tuberculosis (Mtb) strains. This study examines the growth kinetics and drug susceptibility of Mtb strains from different lineages in Ethiopia to understand their impact on disease management. Mtb strains, including sub-lineages 4.1.2.1, 4.2.2.2, 4.6.3, lineages 3 and 7, and the reference strain H37Rv (ATCC 27294), were cultured in liquid 7H9 Middlebrook broth. Growth began on day 6 post-inoculation. Sub-lineage 4.1.2.1 showed rapid exponential growth by day 9, reaching the stationary phase by day 15. Sub-lineage 4.1.2.1 followed by sub-lineage 4.2.2.2 had the highest maximum growth concentration (Cmax), indicating enhanced growth efficiency and adaptive traits that may increase their pathogenicity or resistance to host defenses or anti-TB drugs. To support this observation, the minimum inhibitory concentrations (MIC) for first-line anti-TB drugs were assessed for all the studied Mtb strains using the microdilution broth method. While all strains were susceptible, MIC values varied. Sub-lineages 4.1.2.1 and 4.2.2.2 had MIC values matching WHO’s critical concentrations (except for rifampicin). Lineage 3 showed increased sensitivity to rifampicin, isoniazid, and streptomycin, requiring only half the standard concentration. Lineage 7 also exhibited higher sensitivity to rifampicin and streptomycin. These findings highlight the importance of considering lineage-specific differences in Mtb strains for optimizing treatment regimens and improving TB control strategies, particularly in regions with diverse Mtb populations like Ethiopia. |
| format | Article |
| id | doaj-art-d2f4a4a61905444fb724fe02e0a9bbbe |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-d2f4a4a61905444fb724fe02e0a9bbbe2025-02-04T06:31:48ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-02-011510.3389/fmicb.2024.15125801512580Comparative growth kinetics and drug susceptibility of Mycobacterium tuberculosis lineages prevalent in Ethiopia: implications for tuberculosis treatment and managementTesfaye Gebreyohannis Hailemariam0Tesfaye Gebreyohannis Hailemariam1Melaku Tilahun2Abay Atnafu3Tesfaye Gelanew4Tewodros Tariku Gebresilase5Mekdes Alemu Tola6Abaysew Ayele7Shewki Moga Siraj8Workineh Shibeshi9Kidist Bobosha10Liya Wassie11Yonas Hirutu12Ephrem Engidawork13Department of Pharmacology and Clinical Pharmacy, School of Pharmacy, College of Health Sciences, Addis Ababa University, Addis Ababa, EthiopiaArmauer Hansen Research Institute (AHRI), Addis Ababa, EthiopiaArmauer Hansen Research Institute (AHRI), Addis Ababa, EthiopiaArmauer Hansen Research Institute (AHRI), Addis Ababa, EthiopiaArmauer Hansen Research Institute (AHRI), Addis Ababa, EthiopiaArmauer Hansen Research Institute (AHRI), Addis Ababa, EthiopiaArmauer Hansen Research Institute (AHRI), Addis Ababa, EthiopiaArmauer Hansen Research Institute (AHRI), Addis Ababa, EthiopiaEthiopian Public Health Institute, Addis Ababa, EthiopiaDepartment of Pharmacology and Clinical Pharmacy, School of Pharmacy, College of Health Sciences, Addis Ababa University, Addis Ababa, EthiopiaArmauer Hansen Research Institute (AHRI), Addis Ababa, EthiopiaArmauer Hansen Research Institute (AHRI), Addis Ababa, EthiopiaArmauer Hansen Research Institute (AHRI), Addis Ababa, EthiopiaDepartment of Pharmacology and Clinical Pharmacy, School of Pharmacy, College of Health Sciences, Addis Ababa University, Addis Ababa, EthiopiaTuberculosis (TB) remains a global health challenge, with treatment outcomes influenced by the genetic diversity of Mycobacterium tuberculosis (Mtb) strains. This study examines the growth kinetics and drug susceptibility of Mtb strains from different lineages in Ethiopia to understand their impact on disease management. Mtb strains, including sub-lineages 4.1.2.1, 4.2.2.2, 4.6.3, lineages 3 and 7, and the reference strain H37Rv (ATCC 27294), were cultured in liquid 7H9 Middlebrook broth. Growth began on day 6 post-inoculation. Sub-lineage 4.1.2.1 showed rapid exponential growth by day 9, reaching the stationary phase by day 15. Sub-lineage 4.1.2.1 followed by sub-lineage 4.2.2.2 had the highest maximum growth concentration (Cmax), indicating enhanced growth efficiency and adaptive traits that may increase their pathogenicity or resistance to host defenses or anti-TB drugs. To support this observation, the minimum inhibitory concentrations (MIC) for first-line anti-TB drugs were assessed for all the studied Mtb strains using the microdilution broth method. While all strains were susceptible, MIC values varied. Sub-lineages 4.1.2.1 and 4.2.2.2 had MIC values matching WHO’s critical concentrations (except for rifampicin). Lineage 3 showed increased sensitivity to rifampicin, isoniazid, and streptomycin, requiring only half the standard concentration. Lineage 7 also exhibited higher sensitivity to rifampicin and streptomycin. These findings highlight the importance of considering lineage-specific differences in Mtb strains for optimizing treatment regimens and improving TB control strategies, particularly in regions with diverse Mtb populations like Ethiopia.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1512580/fullMycobacterium tuberculosissub-lineagesgrowth kineticsminimum inhibitory concentrationsoptimizing TB treatment |
| spellingShingle | Tesfaye Gebreyohannis Hailemariam Tesfaye Gebreyohannis Hailemariam Melaku Tilahun Abay Atnafu Tesfaye Gelanew Tewodros Tariku Gebresilase Mekdes Alemu Tola Abaysew Ayele Shewki Moga Siraj Workineh Shibeshi Kidist Bobosha Liya Wassie Yonas Hirutu Ephrem Engidawork Comparative growth kinetics and drug susceptibility of Mycobacterium tuberculosis lineages prevalent in Ethiopia: implications for tuberculosis treatment and management Frontiers in Microbiology Mycobacterium tuberculosis sub-lineages growth kinetics minimum inhibitory concentrations optimizing TB treatment |
| title | Comparative growth kinetics and drug susceptibility of Mycobacterium tuberculosis lineages prevalent in Ethiopia: implications for tuberculosis treatment and management |
| title_full | Comparative growth kinetics and drug susceptibility of Mycobacterium tuberculosis lineages prevalent in Ethiopia: implications for tuberculosis treatment and management |
| title_fullStr | Comparative growth kinetics and drug susceptibility of Mycobacterium tuberculosis lineages prevalent in Ethiopia: implications for tuberculosis treatment and management |
| title_full_unstemmed | Comparative growth kinetics and drug susceptibility of Mycobacterium tuberculosis lineages prevalent in Ethiopia: implications for tuberculosis treatment and management |
| title_short | Comparative growth kinetics and drug susceptibility of Mycobacterium tuberculosis lineages prevalent in Ethiopia: implications for tuberculosis treatment and management |
| title_sort | comparative growth kinetics and drug susceptibility of mycobacterium tuberculosis lineages prevalent in ethiopia implications for tuberculosis treatment and management |
| topic | Mycobacterium tuberculosis sub-lineages growth kinetics minimum inhibitory concentrations optimizing TB treatment |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1512580/full |
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