Effects of Hydrogen‐Rich Water on Growth, Redox Homeostasis and Hormonal, Histological and Immune Systems in Rats Exposed to High Cage Density Stress
ABSTRACT Objectives This study investigated the impact of drinking hydrogen‐rich water (HRW) on growth performance, organ weights, thiol/disulphide homeostasis, oxidative status and some hormonal, histopathological and immunohistochemical changes in rats fed in a restricted housing environment. Meth...
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Wiley
2025-03-01
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| Series: | Veterinary Medicine and Science |
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| Online Access: | https://doi.org/10.1002/vms3.70305 |
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| author | Buket Boğa Kuru Mustafa Makav Mushap Kuru Şükran Yediel Aras Ebru Karadağ Sarı Menekşe Bulut Duried Alwazeer Fikret Bektaşoğlu Mükremin Ölmez Turgut Kırmızıbayrak Tyler W. LeBaron |
| author_facet | Buket Boğa Kuru Mustafa Makav Mushap Kuru Şükran Yediel Aras Ebru Karadağ Sarı Menekşe Bulut Duried Alwazeer Fikret Bektaşoğlu Mükremin Ölmez Turgut Kırmızıbayrak Tyler W. LeBaron |
| author_sort | Buket Boğa Kuru |
| collection | DOAJ |
| description | ABSTRACT Objectives This study investigated the impact of drinking hydrogen‐rich water (HRW) on growth performance, organ weights, thiol/disulphide homeostasis, oxidative status and some hormonal, histopathological and immunohistochemical changes in rats fed in a restricted housing environment. Methods The eight groups (each group [male/female] eight rats) comprised two control, two hydrogen, two stress and two stress + hydrogen. All animals were given feed and water ad libitum for 3 months. Control and HRW group rats were calculated according to weight and housed according to the Guide's housing condition. The stress group and stress + HRW group were housed in half the area of the Guide's housing condition according to their weight. The animal's weekly body weights were measured throughout the study. The animals were sacrificed in accordance with ethical rules. Then, biochemical analyses were performed on thyroid‐stimulating hormone (TSH), free triiodothyronine (FT3), free thyroxine (FT4), cortisol, parathyroid hormone (PTH) and calcium (Ca2+), total thiol (TT), native thiol (NT), disulphide, disulphide/TT × 100, disulphide/NT × 100 and NT/TT × 100, malondialdehyde (MDA) and glutathione (GSH). Haematoxylin staining for histopathological and SOD‐2 immunoreactivity was also assessed. Results Results showed that live weight gain was higher in the HRW groups than in the stress group. Oxidant status in biochemical analyses decreased in the stress + HRW group compared to the stress group. TSH decreased in the stress group. FT4, cortisol and Ca2+ increased in the stress group. Conclusions The stress‐related physiological parameters were reduced in the HRW + stress group compared to the stress group. HRW could be suggested when the organism is found in stressful conditions. |
| format | Article |
| id | doaj-art-d2f4987068ac47bf8539bb2d2ad19931 |
| institution | DOAJ |
| issn | 2053-1095 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Wiley |
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| series | Veterinary Medicine and Science |
| spelling | doaj-art-d2f4987068ac47bf8539bb2d2ad199312025-08-20T02:40:42ZengWileyVeterinary Medicine and Science2053-10952025-03-01112n/an/a10.1002/vms3.70305Effects of Hydrogen‐Rich Water on Growth, Redox Homeostasis and Hormonal, Histological and Immune Systems in Rats Exposed to High Cage Density StressBuket Boğa Kuru0Mustafa Makav1Mushap Kuru2Şükran Yediel Aras3Ebru Karadağ Sarı4Menekşe Bulut5Duried Alwazeer6Fikret Bektaşoğlu7Mükremin Ölmez8Turgut Kırmızıbayrak9Tyler W. LeBaron10Department of Animal Breeding and Husbandry Faculty of Veterinary Medicine Kafkas University Kars TürkiyeDepartment of Physiology Faculty of Veterinary Medicine Kafkas University Kars TürkiyeDepartment of Obstetrics and Gynecology Faculty of Veterinary Medicine Kafkas University Kars TürkiyeDepartment of Midwifery Faculty of Health Sciences Kafkas University Kars TürkiyeDepartment of Histology‐Embryology Faculty of Veterinary Medicine Kafkas University Kars TürkiyeDepartment of Food Engineering Faculty of Engineering Iğdır University Iğdır TürkiyeDepartment of Nutrition and Dietetics Faculty of Health Sciences Iğdır University Iğdır TürkiyeDepartment of Animal Breeding and Husbandry Faculty of Veterinary Medicine Kafkas University Kars TürkiyeDepartment of Animal Nutrition and Nutritional Diseases Faculty of Veterinary Medicine Kafkas University Kars TürkiyeDepartment of Animal Breeding and Husbandry Faculty of Veterinary Medicine Kafkas University Kars TürkiyeDepartment of Kinesiology and Outdoor Recreation Southern Utah University Cedar City Utah USAABSTRACT Objectives This study investigated the impact of drinking hydrogen‐rich water (HRW) on growth performance, organ weights, thiol/disulphide homeostasis, oxidative status and some hormonal, histopathological and immunohistochemical changes in rats fed in a restricted housing environment. Methods The eight groups (each group [male/female] eight rats) comprised two control, two hydrogen, two stress and two stress + hydrogen. All animals were given feed and water ad libitum for 3 months. Control and HRW group rats were calculated according to weight and housed according to the Guide's housing condition. The stress group and stress + HRW group were housed in half the area of the Guide's housing condition according to their weight. The animal's weekly body weights were measured throughout the study. The animals were sacrificed in accordance with ethical rules. Then, biochemical analyses were performed on thyroid‐stimulating hormone (TSH), free triiodothyronine (FT3), free thyroxine (FT4), cortisol, parathyroid hormone (PTH) and calcium (Ca2+), total thiol (TT), native thiol (NT), disulphide, disulphide/TT × 100, disulphide/NT × 100 and NT/TT × 100, malondialdehyde (MDA) and glutathione (GSH). Haematoxylin staining for histopathological and SOD‐2 immunoreactivity was also assessed. Results Results showed that live weight gain was higher in the HRW groups than in the stress group. Oxidant status in biochemical analyses decreased in the stress + HRW group compared to the stress group. TSH decreased in the stress group. FT4, cortisol and Ca2+ increased in the stress group. Conclusions The stress‐related physiological parameters were reduced in the HRW + stress group compared to the stress group. HRW could be suggested when the organism is found in stressful conditions.https://doi.org/10.1002/vms3.70305growth performance | hydrogen‐rich water | molecular hydrogen | oxidative status | stress |
| spellingShingle | Buket Boğa Kuru Mustafa Makav Mushap Kuru Şükran Yediel Aras Ebru Karadağ Sarı Menekşe Bulut Duried Alwazeer Fikret Bektaşoğlu Mükremin Ölmez Turgut Kırmızıbayrak Tyler W. LeBaron Effects of Hydrogen‐Rich Water on Growth, Redox Homeostasis and Hormonal, Histological and Immune Systems in Rats Exposed to High Cage Density Stress Veterinary Medicine and Science growth performance | hydrogen‐rich water | molecular hydrogen | oxidative status | stress |
| title | Effects of Hydrogen‐Rich Water on Growth, Redox Homeostasis and Hormonal, Histological and Immune Systems in Rats Exposed to High Cage Density Stress |
| title_full | Effects of Hydrogen‐Rich Water on Growth, Redox Homeostasis and Hormonal, Histological and Immune Systems in Rats Exposed to High Cage Density Stress |
| title_fullStr | Effects of Hydrogen‐Rich Water on Growth, Redox Homeostasis and Hormonal, Histological and Immune Systems in Rats Exposed to High Cage Density Stress |
| title_full_unstemmed | Effects of Hydrogen‐Rich Water on Growth, Redox Homeostasis and Hormonal, Histological and Immune Systems in Rats Exposed to High Cage Density Stress |
| title_short | Effects of Hydrogen‐Rich Water on Growth, Redox Homeostasis and Hormonal, Histological and Immune Systems in Rats Exposed to High Cage Density Stress |
| title_sort | effects of hydrogen rich water on growth redox homeostasis and hormonal histological and immune systems in rats exposed to high cage density stress |
| topic | growth performance | hydrogen‐rich water | molecular hydrogen | oxidative status | stress |
| url | https://doi.org/10.1002/vms3.70305 |
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