Warming increases richness and shapes assemblages of eukaryotic parasitic plankton

Abstract Background Anthropogenic activities have led to a global rise in water temperatures, prompting increased interest in how warming affects infectious disease ecology. While most studies have focused on individual host-parasite systems, there is a gap in understanding the impact of warming on...

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Main Authors: Amruta Rajarajan, Sławek Cerbin, Kingsly C. Beng, Michael T. Monaghan, Justyna Wolinska
Format: Article
Language:English
Published: BMC 2025-06-01
Series:Environmental Microbiome
Online Access:https://doi.org/10.1186/s40793-025-00724-3
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author Amruta Rajarajan
Sławek Cerbin
Kingsly C. Beng
Michael T. Monaghan
Justyna Wolinska
author_facet Amruta Rajarajan
Sławek Cerbin
Kingsly C. Beng
Michael T. Monaghan
Justyna Wolinska
author_sort Amruta Rajarajan
collection DOAJ
description Abstract Background Anthropogenic activities have led to a global rise in water temperatures, prompting increased interest in how warming affects infectious disease ecology. While most studies have focused on individual host-parasite systems, there is a gap in understanding the impact of warming on multi-host, multi-parasite assemblages in natural ecosystems. To address this gap, we investigated freshwater eukaryotic parasite communities in ten natural lakes near Konin, Poland: five artificially heated and five non-heated “control” lakes. Since 1958, the heated lakes have experienced a mean annual temperature increase of 2 °C due to hot water discharge from two adjacent power plants. We collected seasonal environmental DNA (eDNA) samples from surface waters over a two-year period and applied targeted metabarcoding to compare the richness and distribution of eukaryotic parasites across lake types with a focus on protists and fungi. Results Using literature searches and sequence metadata from GenBank, we identified putative parasites which included Alveolates, Stramenopiles, basal Fungi and Ichthyosporeans as well as their associated hosts. Heated lakes harboured distinct parasite assemblages with higher richness of chytrids and aphelids, suggesting thermal preferences among certain freshwater microeukaryotic parasites. Other groups exhibited clear seasonal trends with richness of oomycetes peaking in spring and summer, and that of Cryptomycota in winter and autumn. A general linear model revealed a marginally positive correlation between chytrid parasite richness and richness of their green algal, diatom, and dinoflagellate hosts. Post-hoc analyses indicated that heated lakes exhibited greater seasonal variation in chytrid parasite richness and a stronger correlation between host and parasite richness than control lakes. Conclusion These findings demonstrate that warming can induce strong shifts in the richness and assemblages of freshwater microeukaryotic parasites. Using chytrids as a focal group, we additionally demonstrate that warming may amplify seasonal variation in parasite richness and strengthen host-parasite richness relationships.
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spelling doaj-art-d2f2a556fbd04767b07d7130e874050e2025-08-20T03:22:57ZengBMCEnvironmental Microbiome2524-63722025-06-0120111610.1186/s40793-025-00724-3Warming increases richness and shapes assemblages of eukaryotic parasitic planktonAmruta Rajarajan0Sławek Cerbin1Kingsly C. Beng2Michael T. Monaghan3Justyna Wolinska4Department of Evolutionary and Integrative Ecology, Leibniz Institute of Freshwater Ecology and Inland Fisheries (IGB)Department of General Zoology, Faculty of Biology, Adam Mickiewicz UniversityDepartment of Evolutionary and Integrative Ecology, Leibniz Institute of Freshwater Ecology and Inland Fisheries (IGB)Department of Evolutionary and Integrative Ecology, Leibniz Institute of Freshwater Ecology and Inland Fisheries (IGB)Department of Evolutionary and Integrative Ecology, Leibniz Institute of Freshwater Ecology and Inland Fisheries (IGB)Abstract Background Anthropogenic activities have led to a global rise in water temperatures, prompting increased interest in how warming affects infectious disease ecology. While most studies have focused on individual host-parasite systems, there is a gap in understanding the impact of warming on multi-host, multi-parasite assemblages in natural ecosystems. To address this gap, we investigated freshwater eukaryotic parasite communities in ten natural lakes near Konin, Poland: five artificially heated and five non-heated “control” lakes. Since 1958, the heated lakes have experienced a mean annual temperature increase of 2 °C due to hot water discharge from two adjacent power plants. We collected seasonal environmental DNA (eDNA) samples from surface waters over a two-year period and applied targeted metabarcoding to compare the richness and distribution of eukaryotic parasites across lake types with a focus on protists and fungi. Results Using literature searches and sequence metadata from GenBank, we identified putative parasites which included Alveolates, Stramenopiles, basal Fungi and Ichthyosporeans as well as their associated hosts. Heated lakes harboured distinct parasite assemblages with higher richness of chytrids and aphelids, suggesting thermal preferences among certain freshwater microeukaryotic parasites. Other groups exhibited clear seasonal trends with richness of oomycetes peaking in spring and summer, and that of Cryptomycota in winter and autumn. A general linear model revealed a marginally positive correlation between chytrid parasite richness and richness of their green algal, diatom, and dinoflagellate hosts. Post-hoc analyses indicated that heated lakes exhibited greater seasonal variation in chytrid parasite richness and a stronger correlation between host and parasite richness than control lakes. Conclusion These findings demonstrate that warming can induce strong shifts in the richness and assemblages of freshwater microeukaryotic parasites. Using chytrids as a focal group, we additionally demonstrate that warming may amplify seasonal variation in parasite richness and strengthen host-parasite richness relationships.https://doi.org/10.1186/s40793-025-00724-3
spellingShingle Amruta Rajarajan
Sławek Cerbin
Kingsly C. Beng
Michael T. Monaghan
Justyna Wolinska
Warming increases richness and shapes assemblages of eukaryotic parasitic plankton
Environmental Microbiome
title Warming increases richness and shapes assemblages of eukaryotic parasitic plankton
title_full Warming increases richness and shapes assemblages of eukaryotic parasitic plankton
title_fullStr Warming increases richness and shapes assemblages of eukaryotic parasitic plankton
title_full_unstemmed Warming increases richness and shapes assemblages of eukaryotic parasitic plankton
title_short Warming increases richness and shapes assemblages of eukaryotic parasitic plankton
title_sort warming increases richness and shapes assemblages of eukaryotic parasitic plankton
url https://doi.org/10.1186/s40793-025-00724-3
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