Bioprinting-By-Design of Hydrogel-Based Biomaterials for In Situ Skin Tissue Engineering

Burns are one of the most common trauma injuries worldwide and have detrimental effects on the entire body. However, the current standard of care is autologous split thickness skin grafts (STSGs), which induces additional injuries to the patient. Therefore, the development of alternative treatments...

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Main Authors: Alisa Douglas, Yufei Chen, Margarita Elloso, Adam Levschuk, Marc G. Jeschke
Format: Article
Language:English
Published: MDPI AG 2025-02-01
Series:Gels
Subjects:
Online Access:https://www.mdpi.com/2310-2861/11/2/110
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author Alisa Douglas
Yufei Chen
Margarita Elloso
Adam Levschuk
Marc G. Jeschke
author_facet Alisa Douglas
Yufei Chen
Margarita Elloso
Adam Levschuk
Marc G. Jeschke
author_sort Alisa Douglas
collection DOAJ
description Burns are one of the most common trauma injuries worldwide and have detrimental effects on the entire body. However, the current standard of care is autologous split thickness skin grafts (STSGs), which induces additional injuries to the patient. Therefore, the development of alternative treatments to replace traditional STSGs is critical, and bioprinting could be the future of burn care. Specifically, in situ bioprinting offers several advantages in clinical applications compared to conventional in vitro bioprinting, primarily due to its ability to deposit bioink directly onto the wound. This review provides an in-depth discussion of the aspects involved in in situ bioprinting for skin regeneration, including crosslinking mechanisms, properties of natural and synthetic hydrogel-based bioinks, various in situ bioprinting methods, and the clinical translation of in situ bioprinting. The current limitations of in situ bioprinting is the ideal combination of bioink and printing mechanism to allow multi-material dispensing or to produce well-orchestrated constructs in a timely manner in clinical settings. However, extensive ongoing research is focused on addressing these challenges, and they do not diminish the significant potential of in situ bioprinting for skin regeneration.
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spelling doaj-art-d2e72e45d0034542972e3e78e1efae3e2025-08-20T02:45:00ZengMDPI AGGels2310-28612025-02-0111211010.3390/gels11020110Bioprinting-By-Design of Hydrogel-Based Biomaterials for In Situ Skin Tissue EngineeringAlisa Douglas0Yufei Chen1Margarita Elloso2Adam Levschuk3Marc G. Jeschke4Department of School of Biomedical Engineering, McMaster University, Hamilton, ON L8S 4L8, CanadaDavid Braley Research Institute, Hamilton, ON L8L 2X2, CanadaHamilton Health Sciences, Hamilton, ON L8L 0A4, CanadaSchulich School of Medicine and Dentistry, Western University, London, ON N6A 5C1, CanadaDepartment of School of Biomedical Engineering, McMaster University, Hamilton, ON L8S 4L8, CanadaBurns are one of the most common trauma injuries worldwide and have detrimental effects on the entire body. However, the current standard of care is autologous split thickness skin grafts (STSGs), which induces additional injuries to the patient. Therefore, the development of alternative treatments to replace traditional STSGs is critical, and bioprinting could be the future of burn care. Specifically, in situ bioprinting offers several advantages in clinical applications compared to conventional in vitro bioprinting, primarily due to its ability to deposit bioink directly onto the wound. This review provides an in-depth discussion of the aspects involved in in situ bioprinting for skin regeneration, including crosslinking mechanisms, properties of natural and synthetic hydrogel-based bioinks, various in situ bioprinting methods, and the clinical translation of in situ bioprinting. The current limitations of in situ bioprinting is the ideal combination of bioink and printing mechanism to allow multi-material dispensing or to produce well-orchestrated constructs in a timely manner in clinical settings. However, extensive ongoing research is focused on addressing these challenges, and they do not diminish the significant potential of in situ bioprinting for skin regeneration.https://www.mdpi.com/2310-2861/11/2/110hydrogelbioprintingin situburnsskin regenerationbioink
spellingShingle Alisa Douglas
Yufei Chen
Margarita Elloso
Adam Levschuk
Marc G. Jeschke
Bioprinting-By-Design of Hydrogel-Based Biomaterials for In Situ Skin Tissue Engineering
Gels
hydrogel
bioprinting
in situ
burns
skin regeneration
bioink
title Bioprinting-By-Design of Hydrogel-Based Biomaterials for In Situ Skin Tissue Engineering
title_full Bioprinting-By-Design of Hydrogel-Based Biomaterials for In Situ Skin Tissue Engineering
title_fullStr Bioprinting-By-Design of Hydrogel-Based Biomaterials for In Situ Skin Tissue Engineering
title_full_unstemmed Bioprinting-By-Design of Hydrogel-Based Biomaterials for In Situ Skin Tissue Engineering
title_short Bioprinting-By-Design of Hydrogel-Based Biomaterials for In Situ Skin Tissue Engineering
title_sort bioprinting by design of hydrogel based biomaterials for in situ skin tissue engineering
topic hydrogel
bioprinting
in situ
burns
skin regeneration
bioink
url https://www.mdpi.com/2310-2861/11/2/110
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AT yufeichen bioprintingbydesignofhydrogelbasedbiomaterialsforinsituskintissueengineering
AT margaritaelloso bioprintingbydesignofhydrogelbasedbiomaterialsforinsituskintissueengineering
AT adamlevschuk bioprintingbydesignofhydrogelbasedbiomaterialsforinsituskintissueengineering
AT marcgjeschke bioprintingbydesignofhydrogelbasedbiomaterialsforinsituskintissueengineering