Multi-omics reveals the mechanism of vagus nerve stimulation in the treatment of chronic congestive heart failure
Abstract The potential benefits and underlying mechanisms of Vagus Nerve Stimulation (VNS) in the treatment of Chronic Congestive Heart Failure (CHF) remain insufficiently elucidated. Therefore, this study aims to investigate the therapeutic potential and mechanisms of VNS in treating CHF. In this s...
Saved in:
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-06-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-025-04397-3 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849724941457424384 |
|---|---|
| author | Yuchi Hu Songyuan Dai Liling Chen Xuejuan Ma Hao Li Yanan Lu Chuanxin Li Xinyun Chen Hongning Li Yanzhou Lu Yimei Huang Lulu Zhao Baotong Hua Ling Zhao |
| author_facet | Yuchi Hu Songyuan Dai Liling Chen Xuejuan Ma Hao Li Yanan Lu Chuanxin Li Xinyun Chen Hongning Li Yanzhou Lu Yimei Huang Lulu Zhao Baotong Hua Ling Zhao |
| author_sort | Yuchi Hu |
| collection | DOAJ |
| description | Abstract The potential benefits and underlying mechanisms of Vagus Nerve Stimulation (VNS) in the treatment of Chronic Congestive Heart Failure (CHF) remain insufficiently elucidated. Therefore, this study aims to investigate the therapeutic potential and mechanisms of VNS in treating CHF. In this study, models of CHF and VNS were established prior to performing echocardiography, H&E staining, and flow cytometry to evaluate the effects of VNS on pathological damage associated with CHF and myocardial mitochondrial dysfunction. Multi-omics analysis was conducted to identify changes in metabolites, gut microbiota, and gene expression following VNS treatment. Finally, QPCR was applied to measure levels of essential genes at the mRNA levels. VNS effectively enhances cardiac function, improves metabolic processes, and reduces inflammatory factor levels in dogs with CHF. VNS alleviates myocardial pathological damage, prevents the accumulation of ROS, and increases mitochondrial membrane potential. Multi-omics analysis identified five key metabolites, four key microorganisms, and sixty key genes. Furthermore, combined multi-omics analysis revealed key therapeutic targets, including three metabolites, four microorganisms, and sixteen genes. VNS significantly downregulates the mRNA expression levels of FSTL3, TNFRSF12A, and HBEGF. In summary, VNS has demonstrated effectiveness in the treatment of CHF. Key therapeutic targets identified include Kamahine C, FSTL3, TNFRSF12A, and HBEGF. |
| format | Article |
| id | doaj-art-d2d4db9f985c4a47bf3f05c3cfd673c9 |
| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-d2d4db9f985c4a47bf3f05c3cfd673c92025-08-20T03:10:36ZengNature PortfolioScientific Reports2045-23222025-06-0115111310.1038/s41598-025-04397-3Multi-omics reveals the mechanism of vagus nerve stimulation in the treatment of chronic congestive heart failureYuchi Hu0Songyuan Dai1Liling Chen2Xuejuan Ma3Hao Li4Yanan Lu5Chuanxin Li6Xinyun Chen7Hongning Li8Yanzhou Lu9Yimei Huang10Lulu Zhao11Baotong Hua12Ling Zhao13Department of Cardiology, First Affiliated Hospital of Kunming Medical UniversityDepartment of Cardiology, First Affiliated Hospital of Kunming Medical UniversityDepartment of Laboratory Zoology, Kunming Medical UniversityDepartment of Cardiology, First Affiliated Hospital of Kunming Medical UniversityDepartment of Cardiology, First Affiliated Hospital of Kunming Medical UniversityDepartment of Cardiology, First Affiliated Hospital of Kunming Medical UniversityDepartment of Cardiology, First Affiliated Hospital of Kunming Medical UniversityDepartment of Cardiology, First Affiliated Hospital of Kunming Medical UniversityDepartment of Cardiology, First Affiliated Hospital of Kunming Medical UniversityUniversity of South DakotaDepartment of Cardiology, First Affiliated Hospital of Kunming Medical UniversityDepartment of Cardiology, First Affiliated Hospital of Kunming Medical UniversityDepartment of Cardiology, First Affiliated Hospital of Kunming Medical UniversityDepartment of Cardiology, First Affiliated Hospital of Kunming Medical UniversityAbstract The potential benefits and underlying mechanisms of Vagus Nerve Stimulation (VNS) in the treatment of Chronic Congestive Heart Failure (CHF) remain insufficiently elucidated. Therefore, this study aims to investigate the therapeutic potential and mechanisms of VNS in treating CHF. In this study, models of CHF and VNS were established prior to performing echocardiography, H&E staining, and flow cytometry to evaluate the effects of VNS on pathological damage associated with CHF and myocardial mitochondrial dysfunction. Multi-omics analysis was conducted to identify changes in metabolites, gut microbiota, and gene expression following VNS treatment. Finally, QPCR was applied to measure levels of essential genes at the mRNA levels. VNS effectively enhances cardiac function, improves metabolic processes, and reduces inflammatory factor levels in dogs with CHF. VNS alleviates myocardial pathological damage, prevents the accumulation of ROS, and increases mitochondrial membrane potential. Multi-omics analysis identified five key metabolites, four key microorganisms, and sixty key genes. Furthermore, combined multi-omics analysis revealed key therapeutic targets, including three metabolites, four microorganisms, and sixteen genes. VNS significantly downregulates the mRNA expression levels of FSTL3, TNFRSF12A, and HBEGF. In summary, VNS has demonstrated effectiveness in the treatment of CHF. Key therapeutic targets identified include Kamahine C, FSTL3, TNFRSF12A, and HBEGF.https://doi.org/10.1038/s41598-025-04397-3Vagus nerve stimulationChronic congestive heart failureOmicsMechanism |
| spellingShingle | Yuchi Hu Songyuan Dai Liling Chen Xuejuan Ma Hao Li Yanan Lu Chuanxin Li Xinyun Chen Hongning Li Yanzhou Lu Yimei Huang Lulu Zhao Baotong Hua Ling Zhao Multi-omics reveals the mechanism of vagus nerve stimulation in the treatment of chronic congestive heart failure Scientific Reports Vagus nerve stimulation Chronic congestive heart failure Omics Mechanism |
| title | Multi-omics reveals the mechanism of vagus nerve stimulation in the treatment of chronic congestive heart failure |
| title_full | Multi-omics reveals the mechanism of vagus nerve stimulation in the treatment of chronic congestive heart failure |
| title_fullStr | Multi-omics reveals the mechanism of vagus nerve stimulation in the treatment of chronic congestive heart failure |
| title_full_unstemmed | Multi-omics reveals the mechanism of vagus nerve stimulation in the treatment of chronic congestive heart failure |
| title_short | Multi-omics reveals the mechanism of vagus nerve stimulation in the treatment of chronic congestive heart failure |
| title_sort | multi omics reveals the mechanism of vagus nerve stimulation in the treatment of chronic congestive heart failure |
| topic | Vagus nerve stimulation Chronic congestive heart failure Omics Mechanism |
| url | https://doi.org/10.1038/s41598-025-04397-3 |
| work_keys_str_mv | AT yuchihu multiomicsrevealsthemechanismofvagusnervestimulationinthetreatmentofchroniccongestiveheartfailure AT songyuandai multiomicsrevealsthemechanismofvagusnervestimulationinthetreatmentofchroniccongestiveheartfailure AT lilingchen multiomicsrevealsthemechanismofvagusnervestimulationinthetreatmentofchroniccongestiveheartfailure AT xuejuanma multiomicsrevealsthemechanismofvagusnervestimulationinthetreatmentofchroniccongestiveheartfailure AT haoli multiomicsrevealsthemechanismofvagusnervestimulationinthetreatmentofchroniccongestiveheartfailure AT yananlu multiomicsrevealsthemechanismofvagusnervestimulationinthetreatmentofchroniccongestiveheartfailure AT chuanxinli multiomicsrevealsthemechanismofvagusnervestimulationinthetreatmentofchroniccongestiveheartfailure AT xinyunchen multiomicsrevealsthemechanismofvagusnervestimulationinthetreatmentofchroniccongestiveheartfailure AT hongningli multiomicsrevealsthemechanismofvagusnervestimulationinthetreatmentofchroniccongestiveheartfailure AT yanzhoulu multiomicsrevealsthemechanismofvagusnervestimulationinthetreatmentofchroniccongestiveheartfailure AT yimeihuang multiomicsrevealsthemechanismofvagusnervestimulationinthetreatmentofchroniccongestiveheartfailure AT luluzhao multiomicsrevealsthemechanismofvagusnervestimulationinthetreatmentofchroniccongestiveheartfailure AT baotonghua multiomicsrevealsthemechanismofvagusnervestimulationinthetreatmentofchroniccongestiveheartfailure AT lingzhao multiomicsrevealsthemechanismofvagusnervestimulationinthetreatmentofchroniccongestiveheartfailure |