Exploration of Bioactive Compounds, Antioxidant and Antibacterial Properties, and Their Potential Efficacy Against HT29 Cell Lines in <i>Dictyota bartayresiana</i>
This study investigates the rare seaweed alga <i>Dictyota bartayresiana</i> lamour for biological activity. Antioxidant and antibacterial activities were examined. An MTT assay was carried out to examine cytotoxicity activity against colon cancer cells. The HPTLC analysis was performed f...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-05-01
|
| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1660-3397/23/6/224 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849431864960352256 |
|---|---|
| author | Durairaj Swarna Bharathi Andiyappan Boopathy Raja Suganthi Nachimuthu S. Thangavel Karthik Kannan Sengottaiyan Shanmugan Vinaya Tari |
| author_facet | Durairaj Swarna Bharathi Andiyappan Boopathy Raja Suganthi Nachimuthu S. Thangavel Karthik Kannan Sengottaiyan Shanmugan Vinaya Tari |
| author_sort | Durairaj Swarna Bharathi |
| collection | DOAJ |
| description | This study investigates the rare seaweed alga <i>Dictyota bartayresiana</i> lamour for biological activity. Antioxidant and antibacterial activities were examined. An MTT assay was carried out to examine cytotoxicity activity against colon cancer cells. The HPTLC analysis was performed for four different extracts, which exhibited clear flavonoid band formation at 254 nm and 366 nm with varied ranges of R<sub>f</sub> values: methanolic extract (R<sub>f</sub> 0.87), acetone extract (R<sub>f</sub> 0.82), and benzene (R<sub>f</sub> 0.83). Methanolic Extract Fraction One (MEF1) has a distinct band formation at 366 nm, it is shown to have the highest inhibition (6.20 ± 0.53 mm) against <i>Escherichia coli</i>, and the MTT assay reveals that the aqueous extract of <i>Dictyota bartayresiana</i> extract has an IC<sub>50</sub> value of 300 µg/mL. It is divulged that methanolic extract shows the highest phytochemical compound level among the four extracts of <i>Dictyota bartayresiana</i>. A GC/MS analysis was employed to investigate the flavonoid profile of the crude seaweed extract. Although LC/MS is typically preferred for flavonoid analysis due to thermal sensitivity, GC/MS was used in this study owing to time constraints, with optimized conditions to reduce thermal degradation. The GC-MS analysis identified Quinoline and other flavonoids, suggesting potential bioactivity. The cytotoxicity activity of MEF1 shows that the development of a promising drug may be evaluated from a seaweed source. The present study provides excellent insight with the first report of the biologically active compound of <i>Dictyota bartayresiana</i>. |
| format | Article |
| id | doaj-art-d2b8178ca41f4ed08a101a8b4c2f2cfd |
| institution | Kabale University |
| issn | 1660-3397 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj-art-d2b8178ca41f4ed08a101a8b4c2f2cfd2025-08-20T03:27:30ZengMDPI AGMarine Drugs1660-33972025-05-0123622410.3390/md23060224Exploration of Bioactive Compounds, Antioxidant and Antibacterial Properties, and Their Potential Efficacy Against HT29 Cell Lines in <i>Dictyota bartayresiana</i>Durairaj Swarna Bharathi0Andiyappan Boopathy Raja1Suganthi Nachimuthu2S. Thangavel3Karthik Kannan4Sengottaiyan Shanmugan5Vinaya Tari6PG and Research Department of Zoology, Nehru Memorial College, Affiliated to Bharathidasan University, Puthanampatti 621004, Tamil Nadu, IndiaPG and Research Department of Zoology, Nehru Memorial College, Affiliated to Bharathidasan University, Puthanampatti 621004, Tamil Nadu, IndiaPG and Research Department of Physics, Government Arts College (Autonomous), Affiliated to Bharathidasan University, Karur 639005, Tamil Nadu, IndiaDepartment of Physics, C.S.I. Bishop Solomon Doraisawmy College of Arts and Science, Karur 639001, Tamil Nadu, IndiaDepartment of Mechanical Engineering, Advanced Institute of Manufacturing with High-Tech Innovations, National Chung Cheng University, Chia-Yi 621301, TaiwanResearch Centre for Solar Energy, Integrated Research and Discovery, Department of Physics, Koneru Lakshmaiah Education Foundation, Green Fields, Vaddeswaram, Guntur 522502, Andhra Pradesh, IndiaDepartment of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya 60115, East Java, IndonesiaThis study investigates the rare seaweed alga <i>Dictyota bartayresiana</i> lamour for biological activity. Antioxidant and antibacterial activities were examined. An MTT assay was carried out to examine cytotoxicity activity against colon cancer cells. The HPTLC analysis was performed for four different extracts, which exhibited clear flavonoid band formation at 254 nm and 366 nm with varied ranges of R<sub>f</sub> values: methanolic extract (R<sub>f</sub> 0.87), acetone extract (R<sub>f</sub> 0.82), and benzene (R<sub>f</sub> 0.83). Methanolic Extract Fraction One (MEF1) has a distinct band formation at 366 nm, it is shown to have the highest inhibition (6.20 ± 0.53 mm) against <i>Escherichia coli</i>, and the MTT assay reveals that the aqueous extract of <i>Dictyota bartayresiana</i> extract has an IC<sub>50</sub> value of 300 µg/mL. It is divulged that methanolic extract shows the highest phytochemical compound level among the four extracts of <i>Dictyota bartayresiana</i>. A GC/MS analysis was employed to investigate the flavonoid profile of the crude seaweed extract. Although LC/MS is typically preferred for flavonoid analysis due to thermal sensitivity, GC/MS was used in this study owing to time constraints, with optimized conditions to reduce thermal degradation. The GC-MS analysis identified Quinoline and other flavonoids, suggesting potential bioactivity. The cytotoxicity activity of MEF1 shows that the development of a promising drug may be evaluated from a seaweed source. The present study provides excellent insight with the first report of the biologically active compound of <i>Dictyota bartayresiana</i>.https://www.mdpi.com/1660-3397/23/6/224<i>Dictyota barteyresiana</i>HP-TLC analysisGC-MSantioxidant activityantibacterial activityHT 29 cancer cells |
| spellingShingle | Durairaj Swarna Bharathi Andiyappan Boopathy Raja Suganthi Nachimuthu S. Thangavel Karthik Kannan Sengottaiyan Shanmugan Vinaya Tari Exploration of Bioactive Compounds, Antioxidant and Antibacterial Properties, and Their Potential Efficacy Against HT29 Cell Lines in <i>Dictyota bartayresiana</i> Marine Drugs <i>Dictyota barteyresiana</i> HP-TLC analysis GC-MS antioxidant activity antibacterial activity HT 29 cancer cells |
| title | Exploration of Bioactive Compounds, Antioxidant and Antibacterial Properties, and Their Potential Efficacy Against HT29 Cell Lines in <i>Dictyota bartayresiana</i> |
| title_full | Exploration of Bioactive Compounds, Antioxidant and Antibacterial Properties, and Their Potential Efficacy Against HT29 Cell Lines in <i>Dictyota bartayresiana</i> |
| title_fullStr | Exploration of Bioactive Compounds, Antioxidant and Antibacterial Properties, and Their Potential Efficacy Against HT29 Cell Lines in <i>Dictyota bartayresiana</i> |
| title_full_unstemmed | Exploration of Bioactive Compounds, Antioxidant and Antibacterial Properties, and Their Potential Efficacy Against HT29 Cell Lines in <i>Dictyota bartayresiana</i> |
| title_short | Exploration of Bioactive Compounds, Antioxidant and Antibacterial Properties, and Their Potential Efficacy Against HT29 Cell Lines in <i>Dictyota bartayresiana</i> |
| title_sort | exploration of bioactive compounds antioxidant and antibacterial properties and their potential efficacy against ht29 cell lines in i dictyota bartayresiana i |
| topic | <i>Dictyota barteyresiana</i> HP-TLC analysis GC-MS antioxidant activity antibacterial activity HT 29 cancer cells |
| url | https://www.mdpi.com/1660-3397/23/6/224 |
| work_keys_str_mv | AT durairajswarnabharathi explorationofbioactivecompoundsantioxidantandantibacterialpropertiesandtheirpotentialefficacyagainstht29celllinesinidictyotabartayresianai AT andiyappanboopathyraja explorationofbioactivecompoundsantioxidantandantibacterialpropertiesandtheirpotentialefficacyagainstht29celllinesinidictyotabartayresianai AT suganthinachimuthu explorationofbioactivecompoundsantioxidantandantibacterialpropertiesandtheirpotentialefficacyagainstht29celllinesinidictyotabartayresianai AT sthangavel explorationofbioactivecompoundsantioxidantandantibacterialpropertiesandtheirpotentialefficacyagainstht29celllinesinidictyotabartayresianai AT karthikkannan explorationofbioactivecompoundsantioxidantandantibacterialpropertiesandtheirpotentialefficacyagainstht29celllinesinidictyotabartayresianai AT sengottaiyanshanmugan explorationofbioactivecompoundsantioxidantandantibacterialpropertiesandtheirpotentialefficacyagainstht29celllinesinidictyotabartayresianai AT vinayatari explorationofbioactivecompoundsantioxidantandantibacterialpropertiesandtheirpotentialefficacyagainstht29celllinesinidictyotabartayresianai |