In vitro efficacy of photodynamic therapy with toluidine blue for decontamination of resorbable membranes inoculated with Streptococcus mutans
Objectives: This study assessed the efficacy of antimicrobial photodynamic therapy (aPDT) with toluidine blue (TB) for decontamination of resorbable membranes inoculated with Streptococcus mutans (S. mutans). Materials and Methods: In this in vitro study, Mucoderm and Jason resorbable membranes were...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-06-01
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| Series: | Photodiagnosis and Photodynamic Therapy |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1572100025000675 |
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| Summary: | Objectives: This study assessed the efficacy of antimicrobial photodynamic therapy (aPDT) with toluidine blue (TB) for decontamination of resorbable membranes inoculated with Streptococcus mutans (S. mutans). Materials and Methods: In this in vitro study, Mucoderm and Jason resorbable membranes were cut into 15 pieces each, and were inoculated with S. mutans at 105 colony forming units (CFUs)/mL concentration. The membranes were subsequently assigned to 3 subgroups each (n = 5), for decontamination with TB (100 µg/mL) activated by 635 nm laser (60 s) as the experimental group (aPDT), 0.12 % chlorhexidine (CHX) as the positive control, and phosphate buffered saline (PBS) as the negative control. Changes in colony count after the interventions were calculated. Data were analyzed by the Kruskal-Wallis and Dunn's tests (alpha=0.05). Results: In both membrane types, the difference in colony count was significant among the three subgroups (P = 0.002). The lowest colony count was noted in the CHX subgroup, and the highest was recorded in the PBS subgroup. aPDT caused a significant reduction in colony count compared with the negative control group (P = 0.002). Significant differences were found between all three groups in pairwise comparisons (P < 0.05). The efficacy of aPDT was not significantly different for decontamination of the two membrane types (P = 635). Conclusion: aPDT with 635 nm diode laser and TB had optimal efficacy (although inferior to CHX) for decontamination of both Jason and Mucoderm resorbable membranes inoculated with S. mutans. |
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| ISSN: | 1572-1000 |