The transcription factors ADR1 or CAT8 are required for RTG pathway activation and evasion from yeast acetic acid-induced programmed cell death in raffinose
Yeast Saccharomyces cerevisiae grown on glucose undergoes programmed cell death (PCD) induced by acetic acid (AA-PCD), but evades PCD when grown in raffinose. This is due to concomitant relief of carbon catabolite repression (CCR) and activation of mitochondrial retrograde signaling, a mitochondria-...
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Shared Science Publishers OG
2016-12-01
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| Series: | Microbial Cell |
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| Online Access: | http://microbialcell.com/researcharticles/the-transcription-factors-adr1-or-cat8-are-required-for-rtg-pathway-activation-and-evasion-from-yeast-acetic-acid-induced-programmed-cell-death-in-raffinose/ |
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| author | Luna Laera Nicoletta Guaragnella Maša Ždralević Domenico Marzulli Zhengchang Liu Sergio Giannattasio |
| author_facet | Luna Laera Nicoletta Guaragnella Maša Ždralević Domenico Marzulli Zhengchang Liu Sergio Giannattasio |
| author_sort | Luna Laera |
| collection | DOAJ |
| description | Yeast Saccharomyces cerevisiae grown on glucose undergoes programmed cell death (PCD) induced by acetic acid (AA-PCD), but evades PCD when grown in raffinose. This is due to concomitant relief of carbon catabolite repression (CCR) and activation of mitochondrial retrograde signaling, a mitochondria-to-nucleus communication pathway causing up-regulation of various nuclear target genes, such as CIT2, encoding peroxisomal citrate synthase, dependent on the positive regulator RTG2 in response to mitochondrial dysfunction. CCR down-regulates genes mainly involved in mitochondrial respiratory metabolism. In this work, we investigated the relationships between the RTG and CCR pathways in the modulation of AA-PCD sensitivity under glucose repression or de-repression conditions. Yeast single and double mutants lacking RTG2 and/or certain factors regulating carbon source utilization, including MIG1, HXK2, ADR1, CAT8, and HAP4, have been analyzed for their survival and CIT2 expression after acetic acid treatment. ADR1 and CAT8 were identified as positive regulators of RTG-dependent gene transcription. ADR1 and CAT8 interact with RTG2 and with each other in inducing cell resistance to AA-PCD in raffinose and controlling the nature of cell death. In the absence of ADR1 and CAT8, AA-PCD evasion is acquired through activation of an alternative factor/pathway repressed by RTG2, suggesting that RTG2 may play a function in promoting necrotic cell death in repressing conditions when RTG pathway is inactive. Moreover, our data show that simultaneous mitochondrial retrograde pathway activation and SNF1-dependent relief of CCR have a key role in central carbon metabolism reprogramming which modulates the yeast acetic acid-stress response. |
| format | Article |
| id | doaj-art-d2af0e97388d48c6949f780580ca9750 |
| institution | DOAJ |
| issn | 2311-2638 |
| language | English |
| publishDate | 2016-12-01 |
| publisher | Shared Science Publishers OG |
| record_format | Article |
| series | Microbial Cell |
| spelling | doaj-art-d2af0e97388d48c6949f780580ca97502025-08-20T02:52:37ZengShared Science Publishers OGMicrobial Cell2311-26382016-12-0131262163110.15698/mic2016.12.549The transcription factors ADR1 or CAT8 are required for RTG pathway activation and evasion from yeast acetic acid-induced programmed cell death in raffinoseLuna Laera0Nicoletta Guaragnella1Maša Ždralević2Domenico Marzulli3Zhengchang LiuSergio Giannattasio4National Research Council of Italy, Institute of Biomembranes and Bioenergetics, Bari, Italy.National Research Council of Italy, Institute of Biomembranes and Bioenergetics, Bari, Italy.National Research Council of Italy, Institute of Biomembranes and Bioenergetics, Bari, Italy.National Research Council of Italy, Institute of Biomembranes and Bioenergetics, Bari, Italy.National Research Council of Italy, Institute of Biomembranes and Bioenergetics, Bari, Italy.Yeast Saccharomyces cerevisiae grown on glucose undergoes programmed cell death (PCD) induced by acetic acid (AA-PCD), but evades PCD when grown in raffinose. This is due to concomitant relief of carbon catabolite repression (CCR) and activation of mitochondrial retrograde signaling, a mitochondria-to-nucleus communication pathway causing up-regulation of various nuclear target genes, such as CIT2, encoding peroxisomal citrate synthase, dependent on the positive regulator RTG2 in response to mitochondrial dysfunction. CCR down-regulates genes mainly involved in mitochondrial respiratory metabolism. In this work, we investigated the relationships between the RTG and CCR pathways in the modulation of AA-PCD sensitivity under glucose repression or de-repression conditions. Yeast single and double mutants lacking RTG2 and/or certain factors regulating carbon source utilization, including MIG1, HXK2, ADR1, CAT8, and HAP4, have been analyzed for their survival and CIT2 expression after acetic acid treatment. ADR1 and CAT8 were identified as positive regulators of RTG-dependent gene transcription. ADR1 and CAT8 interact with RTG2 and with each other in inducing cell resistance to AA-PCD in raffinose and controlling the nature of cell death. In the absence of ADR1 and CAT8, AA-PCD evasion is acquired through activation of an alternative factor/pathway repressed by RTG2, suggesting that RTG2 may play a function in promoting necrotic cell death in repressing conditions when RTG pathway is inactive. Moreover, our data show that simultaneous mitochondrial retrograde pathway activation and SNF1-dependent relief of CCR have a key role in central carbon metabolism reprogramming which modulates the yeast acetic acid-stress response.http://microbialcell.com/researcharticles/the-transcription-factors-adr1-or-cat8-are-required-for-rtg-pathway-activation-and-evasion-from-yeast-acetic-acid-induced-programmed-cell-death-in-raffinose/yeastprogrammed cell deathmitochondrial retrograde pathwayglucosecarbon catabolite repressionacetic acid |
| spellingShingle | Luna Laera Nicoletta Guaragnella Maša Ždralević Domenico Marzulli Zhengchang Liu Sergio Giannattasio The transcription factors ADR1 or CAT8 are required for RTG pathway activation and evasion from yeast acetic acid-induced programmed cell death in raffinose Microbial Cell yeast programmed cell death mitochondrial retrograde pathway glucose carbon catabolite repression acetic acid |
| title | The transcription factors ADR1 or CAT8 are required for RTG pathway activation and evasion from yeast acetic acid-induced programmed cell death in raffinose |
| title_full | The transcription factors ADR1 or CAT8 are required for RTG pathway activation and evasion from yeast acetic acid-induced programmed cell death in raffinose |
| title_fullStr | The transcription factors ADR1 or CAT8 are required for RTG pathway activation and evasion from yeast acetic acid-induced programmed cell death in raffinose |
| title_full_unstemmed | The transcription factors ADR1 or CAT8 are required for RTG pathway activation and evasion from yeast acetic acid-induced programmed cell death in raffinose |
| title_short | The transcription factors ADR1 or CAT8 are required for RTG pathway activation and evasion from yeast acetic acid-induced programmed cell death in raffinose |
| title_sort | transcription factors adr1 or cat8 are required for rtg pathway activation and evasion from yeast acetic acid induced programmed cell death in raffinose |
| topic | yeast programmed cell death mitochondrial retrograde pathway glucose carbon catabolite repression acetic acid |
| url | http://microbialcell.com/researcharticles/the-transcription-factors-adr1-or-cat8-are-required-for-rtg-pathway-activation-and-evasion-from-yeast-acetic-acid-induced-programmed-cell-death-in-raffinose/ |
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