Widespread anticoagulant resistance in house mice (Mus musculus musculus) linked to the Tyr139Phe mutation in the Czech Republic
Abstract Despite the widespread use of anticoagulant rodenticides in baits for controlling commensal rodent pests, their application is problematic due to secondary intoxication and increasing resistance. In contrast to studies on Western European house mice (Mus musculus domesticus), few resistance...
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Nature Portfolio
2025-01-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-85447-8 |
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| author | Marcela Frankova Zuzana Starostova Radek Aulicky Vaclav Stejskal |
| author_facet | Marcela Frankova Zuzana Starostova Radek Aulicky Vaclav Stejskal |
| author_sort | Marcela Frankova |
| collection | DOAJ |
| description | Abstract Despite the widespread use of anticoagulant rodenticides in baits for controlling commensal rodent pests, their application is problematic due to secondary intoxication and increasing resistance. In contrast to studies on Western European house mice (Mus musculus domesticus), few resistance studies have focused on Eastern European house mice (M. musculus musculus), which have a western distribution boundary in the Czech Republic. This study newly analysed the VKORC1 gene in M. m. musculus field populations from Czech farms and grain stores and identified a nonsynonymous mutation Tyr139Phe. This mutation was common throughout the Czech Republic and was present in 80.2% of the 86 individuals sampled. Additionally, all individuals exhibited a genotype with three synonymous mutations specific to the subspecies M. m. musculus. The functional (mortality–survival) response of the Tyr139Phe mutation was validated in a laboratory choice feeding test using bromadiolone-based bait, where all resistant homozygous individuals survived, while all susceptible mice died, with a mean survival of 6.9 days. |
| format | Article |
| id | doaj-art-d2aa726a52a745a2b3e160e276087c55 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-d2aa726a52a745a2b3e160e276087c552025-01-12T12:23:33ZengNature PortfolioScientific Reports2045-23222025-01-011511910.1038/s41598-025-85447-8Widespread anticoagulant resistance in house mice (Mus musculus musculus) linked to the Tyr139Phe mutation in the Czech RepublicMarcela Frankova0Zuzana Starostova1Radek Aulicky2Vaclav Stejskal3Czech Agrifood Research CenterDepartment of Zoology, Faculty of Science, Charles UniversityCzech Agrifood Research CenterCzech Agrifood Research CenterAbstract Despite the widespread use of anticoagulant rodenticides in baits for controlling commensal rodent pests, their application is problematic due to secondary intoxication and increasing resistance. In contrast to studies on Western European house mice (Mus musculus domesticus), few resistance studies have focused on Eastern European house mice (M. musculus musculus), which have a western distribution boundary in the Czech Republic. This study newly analysed the VKORC1 gene in M. m. musculus field populations from Czech farms and grain stores and identified a nonsynonymous mutation Tyr139Phe. This mutation was common throughout the Czech Republic and was present in 80.2% of the 86 individuals sampled. Additionally, all individuals exhibited a genotype with three synonymous mutations specific to the subspecies M. m. musculus. The functional (mortality–survival) response of the Tyr139Phe mutation was validated in a laboratory choice feeding test using bromadiolone-based bait, where all resistant homozygous individuals survived, while all susceptible mice died, with a mean survival of 6.9 days.https://doi.org/10.1038/s41598-025-85447-8Rodent pestsAnticoagulant rodenticidesResistanceVKORC1Mus musculus musculus |
| spellingShingle | Marcela Frankova Zuzana Starostova Radek Aulicky Vaclav Stejskal Widespread anticoagulant resistance in house mice (Mus musculus musculus) linked to the Tyr139Phe mutation in the Czech Republic Scientific Reports Rodent pests Anticoagulant rodenticides Resistance VKORC1 Mus musculus musculus |
| title | Widespread anticoagulant resistance in house mice (Mus musculus musculus) linked to the Tyr139Phe mutation in the Czech Republic |
| title_full | Widespread anticoagulant resistance in house mice (Mus musculus musculus) linked to the Tyr139Phe mutation in the Czech Republic |
| title_fullStr | Widespread anticoagulant resistance in house mice (Mus musculus musculus) linked to the Tyr139Phe mutation in the Czech Republic |
| title_full_unstemmed | Widespread anticoagulant resistance in house mice (Mus musculus musculus) linked to the Tyr139Phe mutation in the Czech Republic |
| title_short | Widespread anticoagulant resistance in house mice (Mus musculus musculus) linked to the Tyr139Phe mutation in the Czech Republic |
| title_sort | widespread anticoagulant resistance in house mice mus musculus musculus linked to the tyr139phe mutation in the czech republic |
| topic | Rodent pests Anticoagulant rodenticides Resistance VKORC1 Mus musculus musculus |
| url | https://doi.org/10.1038/s41598-025-85447-8 |
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