Detection and quantification of Salmonella spp. in poultry slaughterhouses of southern Brazil
Introduction: Salmonella is a major cause of foodborne illness throughout the world. The use of quantitative techniques is important for assessing the risk and determining the capacity of each step of the slaughtering process to decrease or increase bacterial contamination. We aimed to detect and t...
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The Journal of Infection in Developing Countries
2019-05-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/11107 |
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| author | Karen Apellanis Borges Eduarda Boff Martelo Lilian Andriva dos Santos Thales Quedi Furian Isabel C Cisco Luciane Manto Luciana R dos Santos |
| author_facet | Karen Apellanis Borges Eduarda Boff Martelo Lilian Andriva dos Santos Thales Quedi Furian Isabel C Cisco Luciane Manto Luciana R dos Santos |
| author_sort | Karen Apellanis Borges |
| collection | DOAJ |
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Introduction: Salmonella is a major cause of foodborne illness throughout the world. The use of quantitative techniques is important for assessing the risk and determining the capacity of each step of the slaughtering process to decrease or increase bacterial contamination. We aimed to detect and to quantify the presence of Salmonella in Brazilian processing plants by real-time quantitative polymerase chain reaction (qPCR).
Methodology: A total of 139 poultry slaughterhouses samples were collected in order to detect to and quantify Salmonella by qPCR.
Results: Almost all collection points (3/18), except water from pre-chiller tank, carcasses after pre-chiller, and carcasses frozen at -12ºC for 60 days, and 49% (68/139) of samples were positive for Salmonella. Quantification means varied equally among all of the tested sources, and we could not establish any pattern of variation. A large proportion (52.6%) of cloacal swabs was Salmonella-positive. Also, contamination in transport cages was increased after the cleaning process, indicating that the process was ineffective. The overall prevalence in samples obtained during the slaughtering process was 48.9%, and on the whole rinsed carcasses, this proportion was 50%. The detection of Salmonella in frozen carcasses, even after long periods of storage, indicates that the carcasses are a potential source of infection for consumers.
Conclusions: We found that contamination levels remain similar throughout the slaughtering. qPCR proved to be an efficient method for the detection of Salmonella.
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| format | Article |
| id | doaj-art-d2a8248909c04a5daeebbd0a44ebae39 |
| institution | DOAJ |
| issn | 1972-2680 |
| language | English |
| publishDate | 2019-05-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-d2a8248909c04a5daeebbd0a44ebae392025-08-20T02:57:52ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802019-05-01130510.3855/jidc.11107Detection and quantification of Salmonella spp. in poultry slaughterhouses of southern BrazilKaren Apellanis Borges0Eduarda Boff Martelo1Lilian Andriva dos Santos2Thales Quedi Furian3Isabel C Cisco4Luciane Manto5Luciana R dos Santos6Centro de Diagnóstico e Pesquisa em Patologia Aviária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, BrazilFaculdade de Agronomia e Medicina Veterinária, Universidade de Passo Fundo, Passo Fundo, RS, BrazilFaculdade de Agronomia e Medicina Veterinária, Universidade de Passo Fundo, Passo Fundo, RS, BrazilCentro de Diagnóstico e Pesquisa em Patologia Aviária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, BrazilCentro de Diagnóstico e Pesquisa em Patologia Aviária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, BrazilFaculdade de Agronomia e Medicina Veterinária, Universidade de Passo Fundo, Passo Fundo, RS, BrazilFaculdade de Agronomia e Medicina Veterinária, Universidade de Passo Fundo, Passo Fundo, RS, Brazil Introduction: Salmonella is a major cause of foodborne illness throughout the world. The use of quantitative techniques is important for assessing the risk and determining the capacity of each step of the slaughtering process to decrease or increase bacterial contamination. We aimed to detect and to quantify the presence of Salmonella in Brazilian processing plants by real-time quantitative polymerase chain reaction (qPCR). Methodology: A total of 139 poultry slaughterhouses samples were collected in order to detect to and quantify Salmonella by qPCR. Results: Almost all collection points (3/18), except water from pre-chiller tank, carcasses after pre-chiller, and carcasses frozen at -12ºC for 60 days, and 49% (68/139) of samples were positive for Salmonella. Quantification means varied equally among all of the tested sources, and we could not establish any pattern of variation. A large proportion (52.6%) of cloacal swabs was Salmonella-positive. Also, contamination in transport cages was increased after the cleaning process, indicating that the process was ineffective. The overall prevalence in samples obtained during the slaughtering process was 48.9%, and on the whole rinsed carcasses, this proportion was 50%. The detection of Salmonella in frozen carcasses, even after long periods of storage, indicates that the carcasses are a potential source of infection for consumers. Conclusions: We found that contamination levels remain similar throughout the slaughtering. qPCR proved to be an efficient method for the detection of Salmonella. https://jidc.org/index.php/journal/article/view/11107SalmonellaqPCRslaughterhousepoultry |
| spellingShingle | Karen Apellanis Borges Eduarda Boff Martelo Lilian Andriva dos Santos Thales Quedi Furian Isabel C Cisco Luciane Manto Luciana R dos Santos Detection and quantification of Salmonella spp. in poultry slaughterhouses of southern Brazil Journal of Infection in Developing Countries Salmonella qPCR slaughterhouse poultry |
| title | Detection and quantification of Salmonella spp. in poultry slaughterhouses of southern Brazil |
| title_full | Detection and quantification of Salmonella spp. in poultry slaughterhouses of southern Brazil |
| title_fullStr | Detection and quantification of Salmonella spp. in poultry slaughterhouses of southern Brazil |
| title_full_unstemmed | Detection and quantification of Salmonella spp. in poultry slaughterhouses of southern Brazil |
| title_short | Detection and quantification of Salmonella spp. in poultry slaughterhouses of southern Brazil |
| title_sort | detection and quantification of salmonella spp in poultry slaughterhouses of southern brazil |
| topic | Salmonella qPCR slaughterhouse poultry |
| url | https://jidc.org/index.php/journal/article/view/11107 |
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